Eliminating false-positive results in serum tests for neuromuscular autoimmunity

Metha Apiwattanakul, Andrew B McKeon, Sean J Pittock, Thomas J. Kryzer, Vanda A Lennon

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In this study we describe the false-positive frequency in radioimmunoprecipitation assays for muscle acetylcholine receptor (AChR) and neuronal voltage-gated potassium channel (VGKC) autoantibodies, attributable to 125I-ligand immunoprecipitation. Sera were evaluated for AChR autoantibody (n = 34,095) and VGKC autoantibody (n = 11,028). We retested sera that yielded apparently positive results with 1251-ligand with and without detergent-solubilized cation-channel protein and indentified clinically validated fals-positive rates of 0.05% and 1.7% for AchR and VGKC autoantibodies, respectively. Specificity assurance in radioimmunoprecipitation assays requires subtraction of values for 125I-ligand binding.

Original languageEnglish (US)
Pages (from-to)702-704
Number of pages3
JournalMuscle and Nerve
Volume41
Issue number5
DOIs
StatePublished - May 2010

Fingerprint

Autoimmunity
Autoantibodies
Radioimmunoprecipitation Assay
Cholinergic Receptors
Ligands
Serum
Voltage-Gated Potassium Channels
Immunoprecipitation
Detergents
Cations
Muscles
Proteins

Keywords

  • α-bungarotoxin
  • α-dendrotoxin
  • Acetylcholine receptor autoantibody
  • Myasthenia gravis
  • Potassium channel autoantibody

ASJC Scopus subject areas

  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)
  • Physiology

Cite this

Eliminating false-positive results in serum tests for neuromuscular autoimmunity. / Apiwattanakul, Metha; McKeon, Andrew B; Pittock, Sean J; Kryzer, Thomas J.; Lennon, Vanda A.

In: Muscle and Nerve, Vol. 41, No. 5, 05.2010, p. 702-704.

Research output: Contribution to journalArticle

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