Eliminating false-positive results in serum tests for neuromuscular autoimmunity

Metha Apiwattanakul, Andrew McKeon, Sean J. Pittock, Thomas J. Kryzer, Vanda A. Lennon

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

In this study we describe the false-positive frequency in radioimmunoprecipitation assays for muscle acetylcholine receptor (AChR) and neuronal voltage-gated potassium channel (VGKC) autoantibodies, attributable to 125I-ligand immunoprecipitation. Sera were evaluated for AChR autoantibody (n = 34,095) and VGKC autoantibody (n = 11,028). We retested sera that yielded apparently positive results with 1251-ligand with and without detergent-solubilized cation-channel protein and indentified clinically validated fals-positive rates of 0.05% and 1.7% for AchR and VGKC autoantibodies, respectively. Specificity assurance in radioimmunoprecipitation assays requires subtraction of values for 125I-ligand binding.

Original languageEnglish (US)
Pages (from-to)702-704
Number of pages3
JournalMuscle and Nerve
Volume41
Issue number5
DOIs
StatePublished - May 1 2010

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Keywords

  • Acetylcholine receptor autoantibody
  • Myasthenia gravis
  • Potassium channel autoantibody
  • α-bungarotoxin
  • α-dendrotoxin

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)

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