TY - JOUR
T1 - Elevated levels of M(r) 92,000 type IV collagenase during tumor growth in vivo
AU - Sawaya, Raymond
AU - Go, Yoshinori
AU - Kyritisis, Athanassios P.
AU - Uhm, Joon
AU - Venkaiah, Boyapati
AU - Mohanam, Sanjeeva
AU - Gokaslan, Ziya L.
AU - Rao, Jasti S.
N1 - Funding Information:
This work was supported by NCI Grant CA-56792 and BTC Grant CA-16672 (J. S. Rao). We thank Lena Jones for help with preparation of the manuscript and Walter J. Pagel for manuscript review.
PY - 1998/10/20
Y1 - 1998/10/20
N2 - Our previous studies demonstrated that matrix metalloproteinase (MMP-9) levels were significantly higher in human glioblastoma tissue samples than in low-grade brain tumors and normal brain tissue. In the present study, we measured the levels of MMP-2 and MMP-9 during the growth of glial tumors in nude mice by intracerebral injection of glioblastoma cells. Using gelatin zymography, densitometry, and an enzyme-linked immunosorbent assay, we found that the enzyme activity and protein count of MMP-2 and MMP-9 were a respective 3- to 10- and 2- to 30-fold higher in tumors at day 14 and 28 than in normal tissue. Immunohistochemical staining for MMP-9 showed strong immunoreactivity in tumor cells and the staining intensity was much higher at day 28, compared to day 14. These results suggest that upregulation of MMP-9 plays a major role in the glioma tumor growth in vivo.
AB - Our previous studies demonstrated that matrix metalloproteinase (MMP-9) levels were significantly higher in human glioblastoma tissue samples than in low-grade brain tumors and normal brain tissue. In the present study, we measured the levels of MMP-2 and MMP-9 during the growth of glial tumors in nude mice by intracerebral injection of glioblastoma cells. Using gelatin zymography, densitometry, and an enzyme-linked immunosorbent assay, we found that the enzyme activity and protein count of MMP-2 and MMP-9 were a respective 3- to 10- and 2- to 30-fold higher in tumors at day 14 and 28 than in normal tissue. Immunohistochemical staining for MMP-9 showed strong immunoreactivity in tumor cells and the staining intensity was much higher at day 28, compared to day 14. These results suggest that upregulation of MMP-9 plays a major role in the glioma tumor growth in vivo.
UR - http://www.scopus.com/inward/record.url?scp=0032552730&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032552730&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1998.9466
DO - 10.1006/bbrc.1998.9466
M3 - Article
C2 - 9792825
AN - SCOPUS:0032552730
SN - 0006-291X
VL - 251
SP - 632
EP - 636
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -