Elevated levels of M(r) 92,000 type IV collagenase during tumor growth in vivo

Raymond Sawaya, Yoshinori Go, Athanassios P. Kyritisis, Joon Uhm, Boyapati Venkaiah, Sanjeeva Mohanam, Ziya L. Gokaslan, Jasti S. Rao

Research output: Contribution to journalArticle

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Abstract

Our previous studies demonstrated that matrix metalloproteinase (MMP-9) levels were significantly higher in human glioblastoma tissue samples than in low-grade brain tumors and normal brain tissue. In the present study, we measured the levels of MMP-2 and MMP-9 during the growth of glial tumors in nude mice by intracerebral injection of glioblastoma cells. Using gelatin zymography, densitometry, and an enzyme-linked immunosorbent assay, we found that the enzyme activity and protein count of MMP-2 and MMP-9 were a respective 3- to 10- and 2- to 30-fold higher in tumors at day 14 and 28 than in normal tissue. Immunohistochemical staining for MMP-9 showed strong immunoreactivity in tumor cells and the staining intensity was much higher at day 28, compared to day 14. These results suggest that upregulation of MMP-9 plays a major role in the glioma tumor growth in vivo.

Original languageEnglish (US)
Pages (from-to)632-636
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume251
Issue number2
DOIs
StatePublished - Oct 20 1998
Externally publishedYes

Fingerprint

Collagenases
Matrix Metalloproteinases
Tumors
Growth
Neoplasms
Glioblastoma
Tissue
Brain
Staining and Labeling
Densitometry
Matrix Metalloproteinase 9
Immunosorbents
Gelatin
Nude Mice
Brain Neoplasms
Glioma
Neuroglia
Enzyme activity
Up-Regulation
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Sawaya, R., Go, Y., Kyritisis, A. P., Uhm, J., Venkaiah, B., Mohanam, S., ... Rao, J. S. (1998). Elevated levels of M(r) 92,000 type IV collagenase during tumor growth in vivo. Biochemical and Biophysical Research Communications, 251(2), 632-636. https://doi.org/10.1006/bbrc.1998.9466

Elevated levels of M(r) 92,000 type IV collagenase during tumor growth in vivo. / Sawaya, Raymond; Go, Yoshinori; Kyritisis, Athanassios P.; Uhm, Joon; Venkaiah, Boyapati; Mohanam, Sanjeeva; Gokaslan, Ziya L.; Rao, Jasti S.

In: Biochemical and Biophysical Research Communications, Vol. 251, No. 2, 20.10.1998, p. 632-636.

Research output: Contribution to journalArticle

Sawaya, R, Go, Y, Kyritisis, AP, Uhm, J, Venkaiah, B, Mohanam, S, Gokaslan, ZL & Rao, JS 1998, 'Elevated levels of M(r) 92,000 type IV collagenase during tumor growth in vivo', Biochemical and Biophysical Research Communications, vol. 251, no. 2, pp. 632-636. https://doi.org/10.1006/bbrc.1998.9466
Sawaya, Raymond ; Go, Yoshinori ; Kyritisis, Athanassios P. ; Uhm, Joon ; Venkaiah, Boyapati ; Mohanam, Sanjeeva ; Gokaslan, Ziya L. ; Rao, Jasti S. / Elevated levels of M(r) 92,000 type IV collagenase during tumor growth in vivo. In: Biochemical and Biophysical Research Communications. 1998 ; Vol. 251, No. 2. pp. 632-636.
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