Elevated expressions of osteopontin and tenascin C in ascending aortic aneurysms are associated with trileaflet aortic valves as compared with bicuspid aortic valves

Ramanath Majumdar, Dylan V. Miller, Karla V. Ballman, Gopinathan Unnikrishnan, Stephen H. McKellar, Gobinda Sarkar, Raghavakaimal Sreekumar, Mark E. Bolander, Thoralf M. Sundt

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Objective: Ascending aortic aneurysms (AscAAs) are a highly lethal condition whose pathobiology remains to be poorly understood. Although most AscAAs occur in the presence of a trileaflet aortic valve (TAV), a bicuspid aortic valve (BAV) is a common congenital anomaly associated with an increased risk for an AscAA and dissection independent of functional valve pathology but secondary to inherent structural abnormality of the aorta. The objective of this investigation was to compare the patterns of gene expression in aortas between TAV and BAV patients with the aim of identifying markers for AscAAs. Methods: We used microarray analysis to first compare messenger RNA expressions between aneurysmal aortas from TAV patients (n=11) and those from BAV patients (n=11), identified genes overexpressed in TAV aneurysms, and compared expressions of the selected genes among TAV aneurysms, BAV aneurysms, and normal aortas (n=3). Finally, expressions of the selected genes were assessed by immunostaining of aortas from the TAV, BAV, and normal specimens. Results: Two overexpressed genes in the TAV group, osteopontin (OPN) and tenascin C (TNC), were consistently more highly expressed in TAV aneurysms than in BAV aneurysms and normal aortas as determined by real-time reverse transcriptase quantitative polymerase chain reaction and immunohistochemistry. Differential staining revealed that OPN protein was concentrated in the medial smooth muscle and that TNC protein was concentrated around the vasa vasorum. Conclusions: We identified two novel potential markers, OPN and TNC, that are strongly associated with TAV aneurysms. The roles of OPN and TNC in influencing extracellular matrix remodeling in AscAAs warrant further investigation.

Original languageEnglish (US)
Pages (from-to)144-150
Number of pages7
JournalCardiovascular Pathology
Volume16
Issue number3
DOIs
StatePublished - Apr 2007

Fingerprint

Tenascin
Osteopontin
Aortic Aneurysm
Aortic Valve
Aorta
Gene Expression
Vasa Vasorum
Bicuspid Aortic Valve
Microarray Analysis
Reverse Transcriptase Polymerase Chain Reaction
Genes
Extracellular Matrix
Smooth Muscle
Dissection
Real-Time Polymerase Chain Reaction
Proteins
Immunohistochemistry
Pathology
Staining and Labeling

Keywords

  • Aneurysm
  • Ascending aorta
  • Gene expression
  • Immunohistochemistry

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Pathology and Forensic Medicine

Cite this

Elevated expressions of osteopontin and tenascin C in ascending aortic aneurysms are associated with trileaflet aortic valves as compared with bicuspid aortic valves. / Majumdar, Ramanath; Miller, Dylan V.; Ballman, Karla V.; Unnikrishnan, Gopinathan; McKellar, Stephen H.; Sarkar, Gobinda; Sreekumar, Raghavakaimal; Bolander, Mark E.; Sundt, Thoralf M.

In: Cardiovascular Pathology, Vol. 16, No. 3, 04.2007, p. 144-150.

Research output: Contribution to journalArticle

Majumdar, R, Miller, DV, Ballman, KV, Unnikrishnan, G, McKellar, SH, Sarkar, G, Sreekumar, R, Bolander, ME & Sundt, TM 2007, 'Elevated expressions of osteopontin and tenascin C in ascending aortic aneurysms are associated with trileaflet aortic valves as compared with bicuspid aortic valves', Cardiovascular Pathology, vol. 16, no. 3, pp. 144-150. https://doi.org/10.1016/j.carpath.2006.12.001
Majumdar, Ramanath ; Miller, Dylan V. ; Ballman, Karla V. ; Unnikrishnan, Gopinathan ; McKellar, Stephen H. ; Sarkar, Gobinda ; Sreekumar, Raghavakaimal ; Bolander, Mark E. ; Sundt, Thoralf M. / Elevated expressions of osteopontin and tenascin C in ascending aortic aneurysms are associated with trileaflet aortic valves as compared with bicuspid aortic valves. In: Cardiovascular Pathology. 2007 ; Vol. 16, No. 3. pp. 144-150.
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abstract = "Objective: Ascending aortic aneurysms (AscAAs) are a highly lethal condition whose pathobiology remains to be poorly understood. Although most AscAAs occur in the presence of a trileaflet aortic valve (TAV), a bicuspid aortic valve (BAV) is a common congenital anomaly associated with an increased risk for an AscAA and dissection independent of functional valve pathology but secondary to inherent structural abnormality of the aorta. The objective of this investigation was to compare the patterns of gene expression in aortas between TAV and BAV patients with the aim of identifying markers for AscAAs. Methods: We used microarray analysis to first compare messenger RNA expressions between aneurysmal aortas from TAV patients (n=11) and those from BAV patients (n=11), identified genes overexpressed in TAV aneurysms, and compared expressions of the selected genes among TAV aneurysms, BAV aneurysms, and normal aortas (n=3). Finally, expressions of the selected genes were assessed by immunostaining of aortas from the TAV, BAV, and normal specimens. Results: Two overexpressed genes in the TAV group, osteopontin (OPN) and tenascin C (TNC), were consistently more highly expressed in TAV aneurysms than in BAV aneurysms and normal aortas as determined by real-time reverse transcriptase quantitative polymerase chain reaction and immunohistochemistry. Differential staining revealed that OPN protein was concentrated in the medial smooth muscle and that TNC protein was concentrated around the vasa vasorum. Conclusions: We identified two novel potential markers, OPN and TNC, that are strongly associated with TAV aneurysms. The roles of OPN and TNC in influencing extracellular matrix remodeling in AscAAs warrant further investigation.",
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T1 - Elevated expressions of osteopontin and tenascin C in ascending aortic aneurysms are associated with trileaflet aortic valves as compared with bicuspid aortic valves

AU - Majumdar, Ramanath

AU - Miller, Dylan V.

AU - Ballman, Karla V.

AU - Unnikrishnan, Gopinathan

AU - McKellar, Stephen H.

AU - Sarkar, Gobinda

AU - Sreekumar, Raghavakaimal

AU - Bolander, Mark E.

AU - Sundt, Thoralf M.

PY - 2007/4

Y1 - 2007/4

N2 - Objective: Ascending aortic aneurysms (AscAAs) are a highly lethal condition whose pathobiology remains to be poorly understood. Although most AscAAs occur in the presence of a trileaflet aortic valve (TAV), a bicuspid aortic valve (BAV) is a common congenital anomaly associated with an increased risk for an AscAA and dissection independent of functional valve pathology but secondary to inherent structural abnormality of the aorta. The objective of this investigation was to compare the patterns of gene expression in aortas between TAV and BAV patients with the aim of identifying markers for AscAAs. Methods: We used microarray analysis to first compare messenger RNA expressions between aneurysmal aortas from TAV patients (n=11) and those from BAV patients (n=11), identified genes overexpressed in TAV aneurysms, and compared expressions of the selected genes among TAV aneurysms, BAV aneurysms, and normal aortas (n=3). Finally, expressions of the selected genes were assessed by immunostaining of aortas from the TAV, BAV, and normal specimens. Results: Two overexpressed genes in the TAV group, osteopontin (OPN) and tenascin C (TNC), were consistently more highly expressed in TAV aneurysms than in BAV aneurysms and normal aortas as determined by real-time reverse transcriptase quantitative polymerase chain reaction and immunohistochemistry. Differential staining revealed that OPN protein was concentrated in the medial smooth muscle and that TNC protein was concentrated around the vasa vasorum. Conclusions: We identified two novel potential markers, OPN and TNC, that are strongly associated with TAV aneurysms. The roles of OPN and TNC in influencing extracellular matrix remodeling in AscAAs warrant further investigation.

AB - Objective: Ascending aortic aneurysms (AscAAs) are a highly lethal condition whose pathobiology remains to be poorly understood. Although most AscAAs occur in the presence of a trileaflet aortic valve (TAV), a bicuspid aortic valve (BAV) is a common congenital anomaly associated with an increased risk for an AscAA and dissection independent of functional valve pathology but secondary to inherent structural abnormality of the aorta. The objective of this investigation was to compare the patterns of gene expression in aortas between TAV and BAV patients with the aim of identifying markers for AscAAs. Methods: We used microarray analysis to first compare messenger RNA expressions between aneurysmal aortas from TAV patients (n=11) and those from BAV patients (n=11), identified genes overexpressed in TAV aneurysms, and compared expressions of the selected genes among TAV aneurysms, BAV aneurysms, and normal aortas (n=3). Finally, expressions of the selected genes were assessed by immunostaining of aortas from the TAV, BAV, and normal specimens. Results: Two overexpressed genes in the TAV group, osteopontin (OPN) and tenascin C (TNC), were consistently more highly expressed in TAV aneurysms than in BAV aneurysms and normal aortas as determined by real-time reverse transcriptase quantitative polymerase chain reaction and immunohistochemistry. Differential staining revealed that OPN protein was concentrated in the medial smooth muscle and that TNC protein was concentrated around the vasa vasorum. Conclusions: We identified two novel potential markers, OPN and TNC, that are strongly associated with TAV aneurysms. The roles of OPN and TNC in influencing extracellular matrix remodeling in AscAAs warrant further investigation.

KW - Aneurysm

KW - Ascending aorta

KW - Gene expression

KW - Immunohistochemistry

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