Elevated expression of serine protease HtrA1 in preeclampsia and its role in trophoblast cell migration and invasion

Funminiyi Ajayi, Nicholas Kongoasa, Thomas Gaffey, Yan W. Asmann, William J. Watson, Alfonso Baldi, Peeyush Lala, Viji Shridhar, Brian Brost, Jeremy Chien

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Objective: Aberrant expression of developmentally regulated genes during placental development could affect fetal growth and contribute to preeclampsia. Expression of serine protease HtrA1 is developmentally regulated with the highest expression in decidua capsularis, compared with ectoplacental cone, and with the highest expression during later stages of pregnancy, compared with early stages. This study was designed to determine the expression of HtrA1 in placental tissues from control and preeclamptic pregnancies and to determine the effect of HtrA1 expression in trophoblast cell migration and invasion. Study Design: HtrA1 expression was assessed by immunohistochemistry in placentas from gestational age-matched preeclamptic and control pregnancies. HtrA1 expression in extravillous trophoblast cells, HTR-8/SVneo, was assessed by immunoblotting and immunofluorescence microscopy. Finally, the effect of ectopic expression of HtrA1 on cell migration and invasion was determined in HTR-8/SVneo cells. Results: Higher expression of HtrA1 was detected in placental tissues collected from patients with early-onset preeclampsia, compared with those from gestational age-matched control samples. Moreover, ectopic expression of HtrA1 significantly attenuates HTR-8/SVneo cell migration and invasion. Conclusion: Higher expression of HtrA1 is associated with early-onset preeclampsia and may affect trophoblast cell migration and invasion.

Original languageEnglish (US)
Pages (from-to)557.e1-557.e10
JournalAmerican journal of obstetrics and gynecology
Volume199
Issue number5
DOIs
StatePublished - Nov 2008

Keywords

  • HtrA1
  • invasion
  • migration
  • preeclampsia
  • trophoblast

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Fingerprint Dive into the research topics of 'Elevated expression of serine protease HtrA1 in preeclampsia and its role in trophoblast cell migration and invasion'. Together they form a unique fingerprint.

  • Cite this