Elevated expression of serine protease HtrA1 in preeclampsia and its role in trophoblast cell migration and invasion

Funminiyi Ajayi, Nicholas Kongoasa, Thomas Gaffey, Yan Asmann, William J. Watson, Alfonso Baldi, Peeyush Lala, Vijayalakshmi Shridhar, Brian Brost, Jeremy Chien

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Objective: Aberrant expression of developmentally regulated genes during placental development could affect fetal growth and contribute to preeclampsia. Expression of serine protease HtrA1 is developmentally regulated with the highest expression in decidua capsularis, compared with ectoplacental cone, and with the highest expression during later stages of pregnancy, compared with early stages. This study was designed to determine the expression of HtrA1 in placental tissues from control and preeclamptic pregnancies and to determine the effect of HtrA1 expression in trophoblast cell migration and invasion. Study Design: HtrA1 expression was assessed by immunohistochemistry in placentas from gestational age-matched preeclamptic and control pregnancies. HtrA1 expression in extravillous trophoblast cells, HTR-8/SVneo, was assessed by immunoblotting and immunofluorescence microscopy. Finally, the effect of ectopic expression of HtrA1 on cell migration and invasion was determined in HTR-8/SVneo cells. Results: Higher expression of HtrA1 was detected in placental tissues collected from patients with early-onset preeclampsia, compared with those from gestational age-matched control samples. Moreover, ectopic expression of HtrA1 significantly attenuates HTR-8/SVneo cell migration and invasion. Conclusion: Higher expression of HtrA1 is associated with early-onset preeclampsia and may affect trophoblast cell migration and invasion.

Original languageEnglish (US)
JournalAmerican Journal of Obstetrics and Gynecology
Volume199
Issue number5
DOIs
StatePublished - Nov 2008

Fingerprint

Trophoblasts
Serine Proteases
Pre-Eclampsia
Cell Movement
Pregnancy
Gestational Age
Decidua
Placentation
Fetal Development
Fluorescence Microscopy
Immunoblotting
Placenta
Immunohistochemistry
Genes
Ectopic Gene Expression

Keywords

  • HtrA1
  • invasion
  • migration
  • preeclampsia
  • trophoblast

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Elevated expression of serine protease HtrA1 in preeclampsia and its role in trophoblast cell migration and invasion. / Ajayi, Funminiyi; Kongoasa, Nicholas; Gaffey, Thomas; Asmann, Yan; Watson, William J.; Baldi, Alfonso; Lala, Peeyush; Shridhar, Vijayalakshmi; Brost, Brian; Chien, Jeremy.

In: American Journal of Obstetrics and Gynecology, Vol. 199, No. 5, 11.2008.

Research output: Contribution to journalArticle

Ajayi, Funminiyi ; Kongoasa, Nicholas ; Gaffey, Thomas ; Asmann, Yan ; Watson, William J. ; Baldi, Alfonso ; Lala, Peeyush ; Shridhar, Vijayalakshmi ; Brost, Brian ; Chien, Jeremy. / Elevated expression of serine protease HtrA1 in preeclampsia and its role in trophoblast cell migration and invasion. In: American Journal of Obstetrics and Gynecology. 2008 ; Vol. 199, No. 5.
@article{9d63d32f56f74360af5264d79b0e6080,
title = "Elevated expression of serine protease HtrA1 in preeclampsia and its role in trophoblast cell migration and invasion",
abstract = "Objective: Aberrant expression of developmentally regulated genes during placental development could affect fetal growth and contribute to preeclampsia. Expression of serine protease HtrA1 is developmentally regulated with the highest expression in decidua capsularis, compared with ectoplacental cone, and with the highest expression during later stages of pregnancy, compared with early stages. This study was designed to determine the expression of HtrA1 in placental tissues from control and preeclamptic pregnancies and to determine the effect of HtrA1 expression in trophoblast cell migration and invasion. Study Design: HtrA1 expression was assessed by immunohistochemistry in placentas from gestational age-matched preeclamptic and control pregnancies. HtrA1 expression in extravillous trophoblast cells, HTR-8/SVneo, was assessed by immunoblotting and immunofluorescence microscopy. Finally, the effect of ectopic expression of HtrA1 on cell migration and invasion was determined in HTR-8/SVneo cells. Results: Higher expression of HtrA1 was detected in placental tissues collected from patients with early-onset preeclampsia, compared with those from gestational age-matched control samples. Moreover, ectopic expression of HtrA1 significantly attenuates HTR-8/SVneo cell migration and invasion. Conclusion: Higher expression of HtrA1 is associated with early-onset preeclampsia and may affect trophoblast cell migration and invasion.",
keywords = "HtrA1, invasion, migration, preeclampsia, trophoblast",
author = "Funminiyi Ajayi and Nicholas Kongoasa and Thomas Gaffey and Yan Asmann and Watson, {William J.} and Alfonso Baldi and Peeyush Lala and Vijayalakshmi Shridhar and Brian Brost and Jeremy Chien",
year = "2008",
month = "11",
doi = "10.1016/j.ajog.2008.04.046",
language = "English (US)",
volume = "199",
journal = "American Journal of Obstetrics and Gynecology",
issn = "0002-9378",
publisher = "Mosby Inc.",
number = "5",

}

TY - JOUR

T1 - Elevated expression of serine protease HtrA1 in preeclampsia and its role in trophoblast cell migration and invasion

AU - Ajayi, Funminiyi

AU - Kongoasa, Nicholas

AU - Gaffey, Thomas

AU - Asmann, Yan

AU - Watson, William J.

AU - Baldi, Alfonso

AU - Lala, Peeyush

AU - Shridhar, Vijayalakshmi

AU - Brost, Brian

AU - Chien, Jeremy

PY - 2008/11

Y1 - 2008/11

N2 - Objective: Aberrant expression of developmentally regulated genes during placental development could affect fetal growth and contribute to preeclampsia. Expression of serine protease HtrA1 is developmentally regulated with the highest expression in decidua capsularis, compared with ectoplacental cone, and with the highest expression during later stages of pregnancy, compared with early stages. This study was designed to determine the expression of HtrA1 in placental tissues from control and preeclamptic pregnancies and to determine the effect of HtrA1 expression in trophoblast cell migration and invasion. Study Design: HtrA1 expression was assessed by immunohistochemistry in placentas from gestational age-matched preeclamptic and control pregnancies. HtrA1 expression in extravillous trophoblast cells, HTR-8/SVneo, was assessed by immunoblotting and immunofluorescence microscopy. Finally, the effect of ectopic expression of HtrA1 on cell migration and invasion was determined in HTR-8/SVneo cells. Results: Higher expression of HtrA1 was detected in placental tissues collected from patients with early-onset preeclampsia, compared with those from gestational age-matched control samples. Moreover, ectopic expression of HtrA1 significantly attenuates HTR-8/SVneo cell migration and invasion. Conclusion: Higher expression of HtrA1 is associated with early-onset preeclampsia and may affect trophoblast cell migration and invasion.

AB - Objective: Aberrant expression of developmentally regulated genes during placental development could affect fetal growth and contribute to preeclampsia. Expression of serine protease HtrA1 is developmentally regulated with the highest expression in decidua capsularis, compared with ectoplacental cone, and with the highest expression during later stages of pregnancy, compared with early stages. This study was designed to determine the expression of HtrA1 in placental tissues from control and preeclamptic pregnancies and to determine the effect of HtrA1 expression in trophoblast cell migration and invasion. Study Design: HtrA1 expression was assessed by immunohistochemistry in placentas from gestational age-matched preeclamptic and control pregnancies. HtrA1 expression in extravillous trophoblast cells, HTR-8/SVneo, was assessed by immunoblotting and immunofluorescence microscopy. Finally, the effect of ectopic expression of HtrA1 on cell migration and invasion was determined in HTR-8/SVneo cells. Results: Higher expression of HtrA1 was detected in placental tissues collected from patients with early-onset preeclampsia, compared with those from gestational age-matched control samples. Moreover, ectopic expression of HtrA1 significantly attenuates HTR-8/SVneo cell migration and invasion. Conclusion: Higher expression of HtrA1 is associated with early-onset preeclampsia and may affect trophoblast cell migration and invasion.

KW - HtrA1

KW - invasion

KW - migration

KW - preeclampsia

KW - trophoblast

UR - http://www.scopus.com/inward/record.url?scp=54849435449&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=54849435449&partnerID=8YFLogxK

U2 - 10.1016/j.ajog.2008.04.046

DO - 10.1016/j.ajog.2008.04.046

M3 - Article

C2 - 18667191

AN - SCOPUS:54849435449

VL - 199

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

SN - 0002-9378

IS - 5

ER -