Electrophysiological properties of subventricular zone cells in adult mouse brain

Bin Lai, Xiao Ou Mao, Lin Xie, Su-Youne Chang, Zhi Gang Xiong, Kunlin Jin, David A. Greenberg

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The subventricular zone (SVZ) is a principal site of adult neurogenesis and appears to participate in the brain's response to injury. Thus, measures that enhance SVZ neurogenesis may have a role in treatment of neurological disease. To better characterize SVZ cells and identify potential targets for therapeutic intervention, we studied electrophysiological properties of SVZ cells in adult mouse brain slices using patch-clamp techniques. Electrophysiology was correlated with immunohistochemical phenotype by injecting cells with lucifer yellow and by studying transgenic mice carrying green fluorescent protein under control of the doublecortin (DCX) or glial fibrillary acidic protein (GFAP) promoter. We identified five types of cells in the adult mouse SVZ: type 1 cells, with 4-aminopyridine (4-AP)/tetraethylammonium (TEA)-sensitive and CdCl2-sensitive inward currents; type 2 cells, with Ca2+-sensitive K+ and both 4-AP/TEA-sensitive and -insensitive currents; type 3 cells, with 4-AP/TEA-sensitive and -insensitive K+ and small Na+ currents; type 4 cells, with slowly activating, large linear outward current and sustained outward current without fast-inactivating component; and type 5 cells, with a large outward rectifying current with a fast inactivating component. Type 2 and 3 cells expressed DCX, types 4 and 5 cells expressed GFAP, and type 1 cells expressed neither. We propose that SVZ neurogenesis involves a progression of electrophysiological cell phenotypes from types 4 and 5 cells (astrocytes) to type 1 cells (neuronal progenitors) to types 2 and 3 cells (nascent neurons), and that drugs acting on ion channels expressed during neurogenesis might promote therapeutic neurogenesis in the injured brain.

Original languageEnglish (US)
Pages (from-to)96-105
Number of pages10
JournalBrain Research
Volume1340
DOIs
StatePublished - Jun 22 2010
Externally publishedYes

Fingerprint

Lateral Ventricles
Brain
Neurogenesis
4-Aminopyridine
Tetraethylammonium
Glial Fibrillary Acidic Protein
Phenotype
Cadmium Chloride
Electrophysiology
Patch-Clamp Techniques
Green Fluorescent Proteins
Ion Channels
Astrocytes
Transgenic Mice

Keywords

  • Doublecortin
  • Neurogenesis
  • Potassium channels
  • Sodium channels
  • Stroke

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

Electrophysiological properties of subventricular zone cells in adult mouse brain. / Lai, Bin; Mao, Xiao Ou; Xie, Lin; Chang, Su-Youne; Xiong, Zhi Gang; Jin, Kunlin; Greenberg, David A.

In: Brain Research, Vol. 1340, 22.06.2010, p. 96-105.

Research output: Contribution to journalArticle

Lai, Bin ; Mao, Xiao Ou ; Xie, Lin ; Chang, Su-Youne ; Xiong, Zhi Gang ; Jin, Kunlin ; Greenberg, David A. / Electrophysiological properties of subventricular zone cells in adult mouse brain. In: Brain Research. 2010 ; Vol. 1340. pp. 96-105.
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