TY - JOUR
T1 - Electrogenic Na+/HCO3/- cotransporters
T2 - Cloning and physiology
AU - Romero, Michael F.
AU - Boron, Walter F.
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 1999
Y1 - 1999
N2 - Bicarbonate and CO2 comprise the major pH buffer of biological fluids. In the renal proximal tubule most of the filtered HCO3/- is reabsorbed by an electrogenic Na/HCO3 cotransporter located at the basolateral membrane. This Na+ bicarbonate cotransporter (NBC) was recently cloned. This review highlights the recent developments leading to and since the cloning of NBC: NBC expression cloning, protein features, clone physiology, isoforms and genes, mRNA distribution, and protein distribution. With the NBC amino acid sequence 30-35% identical to the anion exchangers (AE1-3), a superfamily of HCO3/- transporters is emerging. Physiologically, NBC is electrogenic, Na+ dependent, HCO3/- dependent, Cl- independent, and inhibited by stilbenes (DIDS and SITS). NBC clones and proteins have been isolated from several tissues (other than kidney) thought to have physiologically distinct HCO3/- transporters. For example, NBC occurs in pancreas, prostate, brain, heart, small and large intestine, stomach, and epididymis. Finally, there are at least two genes that encode NBC proteins. Possible future directions of research are discussed.
AB - Bicarbonate and CO2 comprise the major pH buffer of biological fluids. In the renal proximal tubule most of the filtered HCO3/- is reabsorbed by an electrogenic Na/HCO3 cotransporter located at the basolateral membrane. This Na+ bicarbonate cotransporter (NBC) was recently cloned. This review highlights the recent developments leading to and since the cloning of NBC: NBC expression cloning, protein features, clone physiology, isoforms and genes, mRNA distribution, and protein distribution. With the NBC amino acid sequence 30-35% identical to the anion exchangers (AE1-3), a superfamily of HCO3/- transporters is emerging. Physiologically, NBC is electrogenic, Na+ dependent, HCO3/- dependent, Cl- independent, and inhibited by stilbenes (DIDS and SITS). NBC clones and proteins have been isolated from several tissues (other than kidney) thought to have physiologically distinct HCO3/- transporters. For example, NBC occurs in pancreas, prostate, brain, heart, small and large intestine, stomach, and epididymis. Finally, there are at least two genes that encode NBC proteins. Possible future directions of research are discussed.
KW - HCO/ transport
KW - Homology cloning
KW - Intracellular pH
KW - Na transport
KW - Rat
KW - Xenopus oocyte expression
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U2 - 10.1146/annurev.physiol.61.1.699
DO - 10.1146/annurev.physiol.61.1.699
M3 - Review article
C2 - 10099707
AN - SCOPUS:0032587119
SN - 0066-4278
VL - 61
SP - 699
EP - 723
JO - Annual Review of Physiology
JF - Annual Review of Physiology
ER -