Electroejaculation of chimeric rats

Marina R. Mccoy, Daniel Montonye, Elizabeth C. Bryda

Research output: Contribution to journalArticlepeer-review

Abstract

With the advent of genetic engineering of rodents came the need to assess fertility and germline competency, especially in chimeric rodents generated using embryonic stem cells. Traditional methods rely on natural mating and progeny testing, which is time- and cost-intensive. Electroejaculation is a faster method of collecting sperm for genetic analysis and offers the additional benefit of using fewer animals. This column describes a refined electroejaculation technique for chimeric rats using light gas anesthesia and a custom-made platform for sperm collection.

Original languageEnglish (US)
Pages (from-to)203-205
Number of pages3
JournalLab Animal
Volume42
Issue number6
DOIs
StatePublished - Jun 2013

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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