TY - JOUR
T1 - Electrochemical release of hepatocyte-on-hydrogel microstructures from ITO substrates
AU - Shah, Sunny S.
AU - Kim, Mihye
AU - Foster, Elena
AU - Vu, Tam
AU - Patel, Dipali
AU - Chen, Li Jung
AU - Verkhoturov, Stanislav V.
AU - Schweikert, Emile
AU - Tae, Giyoong
AU - Revzin, Alexander
N1 - Funding Information:
Acknowledgments We thank Dr. Michael Howland for critical review of the manuscript. This work was supported by an NIH grant (DK079977) awarded to AR. SSS was supported by a predoctoral fellowship (EB003827) and by grant number T32-GM08799 from NIGMS-NIH. This work was also partially supported by The Korean Federation of Science and Technology Societies Grant funded by Korea Goverment (MEST, Basic Research Promotion Fund) awarded to GT. In addition, we acknowledge support from the Korean Federation of Science and Technology Societies Grant funded by Korea Goverment (MEST), Basic Research Promotion Fund. EAS acknowledges support from NSF grant CHE 0750377.
PY - 2012/2
Y1 - 2012/2
N2 - This paper describes a novel platform that utilizes micropatterning and electrochemistry to release cells-onhydrogel microstructures from conductive indium tin oxide (ITO) substrates. In this approach, UV photopolymerization was employed to micropattern heparin-based hydrogels onto glass substrates containing ITO electrodes. ITO/glass substrates were first functionalized with acrylated silane to promote attachment of hydrogel structures. The surfaces containing hydrogel micropatterns were further functionalized with poly (ethylene glycol) thiol, rendering the regions around the hydrogel structures non-fouling to proteins and cells. After incubating surfaces with collagen (I) , primary rat hepatocytes were shown to selectively attach on top of the hydrogel and not on surrounding glass/ITO regions. Electrical activation of specific ITO electrodes (?1.8 V vs. Ag/AgCl reference) was then used to release cells-onhydrogel microstructures from the substrate. Immunostaining and reverse transcription polymerase chain reaction analysis of albumin, an important indicator of hepatic function, showed that the hepatocyte-on-hydrogel microstructures released from the surface maintained their function at levels similar to hepatocytes remaining on the culture substrate. In the future, switchable conductive substrates described here may be to collect cell samples at different time points and may also be used for harvesting cellcarrying vehicles for transplantation studies.
AB - This paper describes a novel platform that utilizes micropatterning and electrochemistry to release cells-onhydrogel microstructures from conductive indium tin oxide (ITO) substrates. In this approach, UV photopolymerization was employed to micropattern heparin-based hydrogels onto glass substrates containing ITO electrodes. ITO/glass substrates were first functionalized with acrylated silane to promote attachment of hydrogel structures. The surfaces containing hydrogel micropatterns were further functionalized with poly (ethylene glycol) thiol, rendering the regions around the hydrogel structures non-fouling to proteins and cells. After incubating surfaces with collagen (I) , primary rat hepatocytes were shown to selectively attach on top of the hydrogel and not on surrounding glass/ITO regions. Electrical activation of specific ITO electrodes (?1.8 V vs. Ag/AgCl reference) was then used to release cells-onhydrogel microstructures from the substrate. Immunostaining and reverse transcription polymerase chain reaction analysis of albumin, an important indicator of hepatic function, showed that the hepatocyte-on-hydrogel microstructures released from the surface maintained their function at levels similar to hepatocytes remaining on the culture substrate. In the future, switchable conductive substrates described here may be to collect cell samples at different time points and may also be used for harvesting cellcarrying vehicles for transplantation studies.
KW - Cell sorting
KW - Electrochemical release
KW - Heparin-based hydrogel
KW - Hepatocytes
KW - Micropatterned surfaces
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U2 - 10.1007/s00216-011-5613-z
DO - 10.1007/s00216-011-5613-z
M3 - Article
C2 - 22203370
AN - SCOPUS:84858698837
SN - 1618-2642
VL - 402
SP - 1847
EP - 1856
JO - Analytical and bioanalytical chemistry
JF - Analytical and bioanalytical chemistry
IS - 5
ER -