Electrochemical biosensors for on-chip detection of oxidative stress from cells

James Enomoto, Zimple Matharu, Alexander Revzin

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

The production of reactive oxygen species (ROS) in the body has been shown to play a significant role in the development and progression of numerous diseases. This makes it important to develop a method of detection for hydrogen peroxide (H2O2), the most stable ROS. Several methods such as the use of fluorescent probes and electrochemistry have been utilized in the past to detect the imbalance in ROS levels generated from injured or stimulated cells. An imbalance in the levels of ROS leads to a state of oxidative stress within the body. Different enzymes such as horseradish peroxidase (HRP) and superoxide dismutase have been used in the detection of ROS. HRP is commonly used as the biorecognition element in many H2O2 sensors. Researchers have looked into immobilizing these enzymes onto carbon nanotubes and nanoparticles to increase sensor sensitivity. In this chapter, we present experimental procedures to perform electrochemical quantification of H 2O2, one of the major ROS release from injured cells (macrophages and hepatocytes).

Original languageEnglish (US)
Title of host publicationHydrogen Peroxide and Cell Signaling, Part A
EditorsEnrique Cadenas, Lester Packer
Pages107-121
Number of pages15
DOIs
StatePublished - Jul 10 2013
Externally publishedYes

Publication series

NameMethods in Enzymology
Volume526
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Fingerprint

Oxidative stress
Biosensing Techniques
Biosensors
Reactive Oxygen Species
Oxidative Stress
Horseradish Peroxidase
Electrochemistry
Carbon Nanotubes
Macrophages
Sensors
Enzymes
Fluorescent Dyes
Nanoparticles
Hydrogen Peroxide
Superoxide Dismutase
Disease Progression
Hepatocytes
Research Personnel

Keywords

  • electrochemical biosensor
  • electrodes cell analysis
  • hydrogel microstructures enzyme
  • Oxidative stress cell injury

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Enomoto, J., Matharu, Z., & Revzin, A. (2013). Electrochemical biosensors for on-chip detection of oxidative stress from cells. In E. Cadenas, & L. Packer (Eds.), Hydrogen Peroxide and Cell Signaling, Part A (pp. 107-121). (Methods in Enzymology; Vol. 526). https://doi.org/10.1016/B978-0-12-405883-5.00006-5

Electrochemical biosensors for on-chip detection of oxidative stress from cells. / Enomoto, James; Matharu, Zimple; Revzin, Alexander.

Hydrogen Peroxide and Cell Signaling, Part A. ed. / Enrique Cadenas; Lester Packer. 2013. p. 107-121 (Methods in Enzymology; Vol. 526).

Research output: Chapter in Book/Report/Conference proceedingChapter

Enomoto, J, Matharu, Z & Revzin, A 2013, Electrochemical biosensors for on-chip detection of oxidative stress from cells. in E Cadenas & L Packer (eds), Hydrogen Peroxide and Cell Signaling, Part A. Methods in Enzymology, vol. 526, pp. 107-121. https://doi.org/10.1016/B978-0-12-405883-5.00006-5
Enomoto J, Matharu Z, Revzin A. Electrochemical biosensors for on-chip detection of oxidative stress from cells. In Cadenas E, Packer L, editors, Hydrogen Peroxide and Cell Signaling, Part A. 2013. p. 107-121. (Methods in Enzymology). https://doi.org/10.1016/B978-0-12-405883-5.00006-5
Enomoto, James ; Matharu, Zimple ; Revzin, Alexander. / Electrochemical biosensors for on-chip detection of oxidative stress from cells. Hydrogen Peroxide and Cell Signaling, Part A. editor / Enrique Cadenas ; Lester Packer. 2013. pp. 107-121 (Methods in Enzymology).
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