Electrical activity modulates growth cone guidance by diffusible factors

Guo Li Ming; John Henley; Marc Tessier-Lavigne; Hong Jun Song; Mu Ming Poo

doi:10.1016/S0896-6273(01)00217-3

Electrical activity modulates growth cone guidance by diffusible factors

Guo Li Ming, John Henley, Marc Tessier-Lavigne, Hong Jun Song, Mu Ming Poo

Neurosurgery

Research output: Contribution to journal › Article › peer-review

200 Scopus citations

Abstract

Brief periods of electrical stimulation of cultured Xenopus spinal neurons resulted in a marked alteration in the turning responses of the growth cone induced by gradients of attractive or repulsive guidance cues. Netrin-1-induced attraction was enhanced, and the repulsion induced by myelin-associated glycoprotein (MAG) or myelin membrane fragments was converted to attraction. The effect required the presence of extracellular Ca²⁺ during electrical stimulation and appeared to be mediated by an elevation of both cytoplasmic Ca²⁺ and cAMP. Thus, electrical activity may influence the axonal path finding of developing neurons, and intermittent electrical stimulation may be effective in promoting nerve regeneration after injury.

Original language	English (US)
Pages (from-to)	441-452
Number of pages	12
Journal	Neuron
Volume	29
Issue number	2
DOIs	https://doi.org/10.1016/S0896-6273(01)00217-3
State	Published - 2001

ASJC Scopus subject areas

Neuroscience(all)

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@article{c73ca3ff765343fcb1eb24706d24fdb8,

title = "Electrical activity modulates growth cone guidance by diffusible factors",

abstract = "Brief periods of electrical stimulation of cultured Xenopus spinal neurons resulted in a marked alteration in the turning responses of the growth cone induced by gradients of attractive or repulsive guidance cues. Netrin-1-induced attraction was enhanced, and the repulsion induced by myelin-associated glycoprotein (MAG) or myelin membrane fragments was converted to attraction. The effect required the presence of extracellular Ca2+ during electrical stimulation and appeared to be mediated by an elevation of both cytoplasmic Ca2+ and cAMP. Thus, electrical activity may influence the axonal path finding of developing neurons, and intermittent electrical stimulation may be effective in promoting nerve regeneration after injury.",

author = "Ming, {Guo Li} and John Henley and Marc Tessier-Lavigne and Song, {Hong Jun} and Poo, {Mu Ming}",

note = "Funding Information: This work was supported by a grant from the National Institutes of Health (NIH) to M.-m. P. (NS37831). J. H. was supported by a fellowship from the NIH. H.-j. S. is an associate and M. T.-L. is an Investigator of the Howard Hughes Medical Institute. We thank L. McKerracher for the gift of myelin fragments and recombinant MAG.",

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AU - Ming, Guo Li

AU - Henley, John

AU - Tessier-Lavigne, Marc

AU - Song, Hong Jun

AU - Poo, Mu Ming

N1 - Funding Information: This work was supported by a grant from the National Institutes of Health (NIH) to M.-m. P. (NS37831). J. H. was supported by a fellowship from the NIH. H.-j. S. is an associate and M. T.-L. is an Investigator of the Howard Hughes Medical Institute. We thank L. McKerracher for the gift of myelin fragments and recombinant MAG.

PY - 2001

Y1 - 2001

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AB - Brief periods of electrical stimulation of cultured Xenopus spinal neurons resulted in a marked alteration in the turning responses of the growth cone induced by gradients of attractive or repulsive guidance cues. Netrin-1-induced attraction was enhanced, and the repulsion induced by myelin-associated glycoprotein (MAG) or myelin membrane fragments was converted to attraction. The effect required the presence of extracellular Ca2+ during electrical stimulation and appeared to be mediated by an elevation of both cytoplasmic Ca2+ and cAMP. Thus, electrical activity may influence the axonal path finding of developing neurons, and intermittent electrical stimulation may be effective in promoting nerve regeneration after injury.

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