Abstract
Brief periods of electrical stimulation of cultured Xenopus spinal neurons resulted in a marked alteration in the turning responses of the growth cone induced by gradients of attractive or repulsive guidance cues. Netrin-1-induced attraction was enhanced, and the repulsion induced by myelin-associated glycoprotein (MAG) or myelin membrane fragments was converted to attraction. The effect required the presence of extracellular Ca2+ during electrical stimulation and appeared to be mediated by an elevation of both cytoplasmic Ca2+ and cAMP. Thus, electrical activity may influence the axonal path finding of developing neurons, and intermittent electrical stimulation may be effective in promoting nerve regeneration after injury.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 441-452 |
| Number of pages | 12 |
| Journal | Neuron |
| Volume | 29 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2001 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Electrical activity modulates growth cone guidance by diffusible factors. / Ming, Guo Li; Henley, John R; Tessier-Lavigne, Marc; Song, Hong Jun; Poo, Mu Ming.
In: Neuron, Vol. 29, No. 2, 2001, p. 441-452.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Electrical activity modulates growth cone guidance by diffusible factors
AU - Ming, Guo Li
AU - Henley, John R
AU - Tessier-Lavigne, Marc
AU - Song, Hong Jun
AU - Poo, Mu Ming
PY - 2001
Y1 - 2001
N2 - Brief periods of electrical stimulation of cultured Xenopus spinal neurons resulted in a marked alteration in the turning responses of the growth cone induced by gradients of attractive or repulsive guidance cues. Netrin-1-induced attraction was enhanced, and the repulsion induced by myelin-associated glycoprotein (MAG) or myelin membrane fragments was converted to attraction. The effect required the presence of extracellular Ca2+ during electrical stimulation and appeared to be mediated by an elevation of both cytoplasmic Ca2+ and cAMP. Thus, electrical activity may influence the axonal path finding of developing neurons, and intermittent electrical stimulation may be effective in promoting nerve regeneration after injury.
AB - Brief periods of electrical stimulation of cultured Xenopus spinal neurons resulted in a marked alteration in the turning responses of the growth cone induced by gradients of attractive or repulsive guidance cues. Netrin-1-induced attraction was enhanced, and the repulsion induced by myelin-associated glycoprotein (MAG) or myelin membrane fragments was converted to attraction. The effect required the presence of extracellular Ca2+ during electrical stimulation and appeared to be mediated by an elevation of both cytoplasmic Ca2+ and cAMP. Thus, electrical activity may influence the axonal path finding of developing neurons, and intermittent electrical stimulation may be effective in promoting nerve regeneration after injury.
UR - http://www.scopus.com/inward/record.url?scp=0035105140&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035105140&partnerID=8YFLogxK
U2 - 10.1016/S0896-6273(01)00217-3
DO - 10.1016/S0896-6273(01)00217-3
M3 - Article
C2 - 11239434
AN - SCOPUS:0035105140
VL - 29
SP - 441
EP - 452
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 2
ER -