Elastase-induced saccular aneurysms in rabbits: A dose-escalation study

David F Kallmes, Naomi H. Fujiwara, Stuart S. Berr, Gregory A. Helm, Harry J. Cloft

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Background and purpose: Reproducible animal models facilitate preclinical assessment of aneurysm therapies. Our purpose was to determine if increased elastase doses enlarge aneurysms and parent arteries. Methods: Rabbit right common carotid artery (CCA) aneurysms were created with distal ligation and intraluminal elastase incubation. Groups were 1) sham (no elastase, n = 3), 2) 25% elastase (10 minutes, n = 9), 3) 50% elastase (10 minutes, n = 7), and 4) 50% elastase (20 minutes, n = 41). Angiography was performed after 14 days. Resultant aneurysm width and height and parent artery diameters were measured and compared with the Student t or Mann-Whitney (Wilcoxon rank sum) test. Results: Proximal segments were enlarged in all elastase subjects and no sham subjects. Mean measurements were significantly smaller in the sham group than in other groups. Aneurysm widths and heights, respectively, were 3.8 mm ± 0.8 and 7.4 mm ± 2.0 in the low-dose group; 3.9 mm ± 1.3 and 8.5 mm ± 3.8, medium-dose group; and 4.1 mm ± 1.1 and 8.7 mm ± 2.6, high-dose group. Differences were not significant. Parent artery widths were 3.5 mm ± 0.7, 3.8 mm ± 0.7, and 4.3 mm ± 1.4 in the low-, medium-, and high-dose groups, respectively; the high-dose group had larger arteries (P = .07). Conclusion: Aneurysms were reliably created and were sized similar to human intracranial aneurysms. Elastase concentration and incubation duration did not affect resultant size. Relatively short incubation (eg, 10 minutes) and 25% elastase can be used to create rabbit aneurysms, especially if dilatation of adjacent parent arteries is to be avoided.

Original languageEnglish (US)
Pages (from-to)295-298
Number of pages4
JournalAmerican Journal of Neuroradiology
Volume23
Issue number2
StatePublished - 2002
Externally publishedYes

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Pancreatic Elastase
Aneurysm
Rabbits
Arteries
Nonparametric Statistics
Common Carotid Artery
Intracranial Aneurysm
Ligation
Dilatation
Angiography
Animal Models
Students

ASJC Scopus subject areas

  • Clinical Neurology
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Kallmes, D. F., Fujiwara, N. H., Berr, S. S., Helm, G. A., & Cloft, H. J. (2002). Elastase-induced saccular aneurysms in rabbits: A dose-escalation study. American Journal of Neuroradiology, 23(2), 295-298.

Elastase-induced saccular aneurysms in rabbits : A dose-escalation study. / Kallmes, David F; Fujiwara, Naomi H.; Berr, Stuart S.; Helm, Gregory A.; Cloft, Harry J.

In: American Journal of Neuroradiology, Vol. 23, No. 2, 2002, p. 295-298.

Research output: Contribution to journalArticle

Kallmes, DF, Fujiwara, NH, Berr, SS, Helm, GA & Cloft, HJ 2002, 'Elastase-induced saccular aneurysms in rabbits: A dose-escalation study', American Journal of Neuroradiology, vol. 23, no. 2, pp. 295-298.
Kallmes, David F ; Fujiwara, Naomi H. ; Berr, Stuart S. ; Helm, Gregory A. ; Cloft, Harry J. / Elastase-induced saccular aneurysms in rabbits : A dose-escalation study. In: American Journal of Neuroradiology. 2002 ; Vol. 23, No. 2. pp. 295-298.
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abstract = "Background and purpose: Reproducible animal models facilitate preclinical assessment of aneurysm therapies. Our purpose was to determine if increased elastase doses enlarge aneurysms and parent arteries. Methods: Rabbit right common carotid artery (CCA) aneurysms were created with distal ligation and intraluminal elastase incubation. Groups were 1) sham (no elastase, n = 3), 2) 25{\%} elastase (10 minutes, n = 9), 3) 50{\%} elastase (10 minutes, n = 7), and 4) 50{\%} elastase (20 minutes, n = 41). Angiography was performed after 14 days. Resultant aneurysm width and height and parent artery diameters were measured and compared with the Student t or Mann-Whitney (Wilcoxon rank sum) test. Results: Proximal segments were enlarged in all elastase subjects and no sham subjects. Mean measurements were significantly smaller in the sham group than in other groups. Aneurysm widths and heights, respectively, were 3.8 mm ± 0.8 and 7.4 mm ± 2.0 in the low-dose group; 3.9 mm ± 1.3 and 8.5 mm ± 3.8, medium-dose group; and 4.1 mm ± 1.1 and 8.7 mm ± 2.6, high-dose group. Differences were not significant. Parent artery widths were 3.5 mm ± 0.7, 3.8 mm ± 0.7, and 4.3 mm ± 1.4 in the low-, medium-, and high-dose groups, respectively; the high-dose group had larger arteries (P = .07). Conclusion: Aneurysms were reliably created and were sized similar to human intracranial aneurysms. Elastase concentration and incubation duration did not affect resultant size. Relatively short incubation (eg, 10 minutes) and 25{\%} elastase can be used to create rabbit aneurysms, especially if dilatation of adjacent parent arteries is to be avoided.",
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