EGF promotes in vivo tumorigenic growth of primary chicken embryo fibroblasts expressing v-myc and enhances in vitro transformation by the v-erbA oncogene

Khashayarsha Khazaie, George Panayotou, Adriano Aguzzi, Jacques Samarut, Louis Gazzolo, Pierre Jurdic

Research output: Contribution to journalArticle

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Abstract

We report that the activation of the endogenous chicken EGF receptor leads to the tumorigenic growth in vivo of early passage chicken embryo fibroblasts (CEFs) that express a nonsarcomagenic oncogene, v-myc. To provide a continuous paracrine source of this growth factor in vivo, we employed irradiated Rat-1 cells which had been stably transfected with a synthetic cDNA to human EGF. Expression of another non-sarcomagenic nuclear oncogene, v-erbA, prones the CEFs to in vitro transformation by EGF, but does not cause EGF dependent tumorigenicity in vivo. The short period of incubation in the in vivo assay employed by our study (10 days), together with the genetic stability of primary chicken embryo fibroblasts, make it very likely that the reported alterations in cellular behaviour are a direct and primary effect of the expression of the relevant oncogenes and their cooperation with the EGF induced response. Dose response and ligand binding assays suggest that the EGF response is transmitted via the chicken c-erbB molecule, which by virtue of its preference for TGF-alfa is distinct from the mammalian EGF receptors studied so far. The level of expression of the endogenous chicken EGF receptor is within the same range as that reported for primary human fibroblasts (5-7 × 103 per cell). The cooperative effect of v-myc with chicken c-erbB probably takes place at a post receptor level, as its expression did not affect the steady state level or affinity for ligand of the chicken EGF receptor.

Original languageEnglish (US)
Pages (from-to)21-28
Number of pages8
JournalOncogene
Volume6
Issue number1
StatePublished - Jan 1991
Externally publishedYes

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erbA Genes
Epidermal Growth Factor
Chickens
Embryonic Structures
Fibroblasts
Growth
Epidermal Growth Factor Receptor
Transforming Growth Factor alpha
myc Genes
In Vitro Techniques
Oncogenes
Intercellular Signaling Peptides and Proteins
Complementary DNA

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

EGF promotes in vivo tumorigenic growth of primary chicken embryo fibroblasts expressing v-myc and enhances in vitro transformation by the v-erbA oncogene. / Khazaie, Khashayarsha; Panayotou, George; Aguzzi, Adriano; Samarut, Jacques; Gazzolo, Louis; Jurdic, Pierre.

In: Oncogene, Vol. 6, No. 1, 01.1991, p. 21-28.

Research output: Contribution to journalArticle

Khazaie, Khashayarsha ; Panayotou, George ; Aguzzi, Adriano ; Samarut, Jacques ; Gazzolo, Louis ; Jurdic, Pierre. / EGF promotes in vivo tumorigenic growth of primary chicken embryo fibroblasts expressing v-myc and enhances in vitro transformation by the v-erbA oncogene. In: Oncogene. 1991 ; Vol. 6, No. 1. pp. 21-28.
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