Efficient surface expression of platelet GPIIb-IIIa requires both subunits

T. E. O'Toole, Joseph C Loftus, E. F. Plow, A. A. Glass, J. R. Harper, M. H. Ginsberg

Research output: Contribution to journalArticle

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Abstract

Platelet membrane GPIIb-IIIa is a member of the integrin family of heterodimeric adhesion receptors. Processing and export of certain leukocyte and melanoma integrins is disrupted in cells lacking one subunit. We found that surface expression of GPIIb-IIIa, measured by fluorescent activated cell sorting or by surface labeling, required cotransfection of both subunits. In contrast, surface expression was not detected when the subunits were transfected individually. Immunoprecipitation of metabolically labeled transfected cells confirmed the presence of comparable levels of intracellular protein in all cases. When both subunits were transfected, post-translational cleavage of Pro-GPIIb to yield GPIIb heavy chain was also seen, while transfection with GPIIb alone resulted in coprecipitation of Pro-GPIIb with a second band that may be an endogenous β subunit. Pro-GPIIb in these transfectants was not processed to yield GPIIb heavy chain. When transfected into COS cells alone, transiently expressed GPIIIa remained intracellular and did not appear to complex with any endogenous proteins. Thus, surface expression of processed GPIIb-IIIa depends on the presence of both subunits; the coordinate reduction of both subunits observed in some cases of Glanzmann's thrombasthenia may result from mutation affecting only one.

Original languageEnglish (US)
Pages (from-to)14-18
Number of pages5
JournalBlood
Volume74
Issue number1
StatePublished - 1989
Externally publishedYes

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Platelets
Blood Platelets
Integrins
Thrombasthenia
Integrin beta3
COS Cells
Immunoprecipitation
Transfection
Melanoma
Proteins
Leukocytes
Coprecipitation
Sorting
Labeling
Mutation
Membranes
Cells
Processing

ASJC Scopus subject areas

  • Hematology

Cite this

O'Toole, T. E., Loftus, J. C., Plow, E. F., Glass, A. A., Harper, J. R., & Ginsberg, M. H. (1989). Efficient surface expression of platelet GPIIb-IIIa requires both subunits. Blood, 74(1), 14-18.

Efficient surface expression of platelet GPIIb-IIIa requires both subunits. / O'Toole, T. E.; Loftus, Joseph C; Plow, E. F.; Glass, A. A.; Harper, J. R.; Ginsberg, M. H.

In: Blood, Vol. 74, No. 1, 1989, p. 14-18.

Research output: Contribution to journalArticle

O'Toole, TE, Loftus, JC, Plow, EF, Glass, AA, Harper, JR & Ginsberg, MH 1989, 'Efficient surface expression of platelet GPIIb-IIIa requires both subunits', Blood, vol. 74, no. 1, pp. 14-18.
O'Toole TE, Loftus JC, Plow EF, Glass AA, Harper JR, Ginsberg MH. Efficient surface expression of platelet GPIIb-IIIa requires both subunits. Blood. 1989;74(1):14-18.
O'Toole, T. E. ; Loftus, Joseph C ; Plow, E. F. ; Glass, A. A. ; Harper, J. R. ; Ginsberg, M. H. / Efficient surface expression of platelet GPIIb-IIIa requires both subunits. In: Blood. 1989 ; Vol. 74, No. 1. pp. 14-18.
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AB - Platelet membrane GPIIb-IIIa is a member of the integrin family of heterodimeric adhesion receptors. Processing and export of certain leukocyte and melanoma integrins is disrupted in cells lacking one subunit. We found that surface expression of GPIIb-IIIa, measured by fluorescent activated cell sorting or by surface labeling, required cotransfection of both subunits. In contrast, surface expression was not detected when the subunits were transfected individually. Immunoprecipitation of metabolically labeled transfected cells confirmed the presence of comparable levels of intracellular protein in all cases. When both subunits were transfected, post-translational cleavage of Pro-GPIIb to yield GPIIb heavy chain was also seen, while transfection with GPIIb alone resulted in coprecipitation of Pro-GPIIb with a second band that may be an endogenous β subunit. Pro-GPIIb in these transfectants was not processed to yield GPIIb heavy chain. When transfected into COS cells alone, transiently expressed GPIIIa remained intracellular and did not appear to complex with any endogenous proteins. Thus, surface expression of processed GPIIb-IIIa depends on the presence of both subunits; the coordinate reduction of both subunits observed in some cases of Glanzmann's thrombasthenia may result from mutation affecting only one.

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