Efficient automated assessment of genetic abnormalities detected by fluorescence in situ hybridization on brush cytology in a Barrett esophagus surveillance population

Agnieszka M. Rygiel, Jantine W.P.M. Van Baal, Francesca Milano, Kenneth K. Wang, Febo J. Ten Kate, Paul Fockens, Wilda D. Rosmolen, Jacques J.G.H.M. Bergman, Maikel P. Peppelenbosch, Kausilia K. Krishnadath

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

BACKGROUND. Automated assessment of genetic abnormalities detected by fluorescence in situ hybridization (FISH) in brush cytology specimens from patients with Barrett esophagus (BE) may enhance the clinical applicability of this methodology. The objectives of this study were to validate a novel, automated, proprietary system (CytoVison SPOT AX) for the assessment of FISH abnormalities in BE brush cytology and, subsequently, to use this automated method for screening of a BE surveillance cohort. METHODS. FISH with DNA probes for chromosomes 9, 17, and Y, and for the 9p21 (p16), 17q11.2 (Her2/neu), and 17p13.1 (p53) loci was applied on brush cytology specimens from a surveillance cohort of 151 patients with BE. Validation of the automated system was performed by comparison of the automated FISH results with manual scores for the first 60 patients. RESULTS. There was 98% concordance between manual and automated FISH analysis with κ values from 0.49 to 1 for the different probes. The loss of 17p13.1 (p53) was observed in only 5% of patients with no dysplasia (ND) and in 9% of patients with low-grade dysplasia (LGD) but increased to 46% in patients with high-grade dysplasia (HGD) (P < .005; Fisher exact test). Chromosomes 9 and 17 were observed in 6% of patients with ND, in 21% of patients with LGD, and in 62% of patients with HGD (P < .05). Ten percent of patients with ND had loss of the Y chromosome, which increased to 27% in patients with HGD (P < .05). The amplification of 17q11.2 (Her2/neu) was detected in 62% of patients with HGD (P < .001). CONCLUSIONS. The current investigation indicated that the CytoVison SPOT AX is an objective, efficient system for the analysis of DNA-FISH on BE brush cytology and is applicable for analyzing large populations of BE patients. In the current study cohort, the loss of 17p13.1 (p53), Y chromosome loss, and polysomy of chromosomes 17 and 9 were correlated with increasing grade of dysplasia in patients with BE.

Original languageEnglish (US)
Pages (from-to)1980-1988
Number of pages9
JournalCancer
Volume109
Issue number10
DOIs
StatePublished - May 15 2007

Keywords

  • Automated fluorescence in situ hybridization analysis
  • Barrett esophagus
  • Brush cytology
  • Cytogenetic abnormalities

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Efficient automated assessment of genetic abnormalities detected by fluorescence in situ hybridization on brush cytology in a Barrett esophagus surveillance population'. Together they form a unique fingerprint.

  • Cite this

    Rygiel, A. M., Van Baal, J. W. P. M., Milano, F., Wang, K. K., Ten Kate, F. J., Fockens, P., Rosmolen, W. D., Bergman, J. J. G. H. M., Peppelenbosch, M. P., & Krishnadath, K. K. (2007). Efficient automated assessment of genetic abnormalities detected by fluorescence in situ hybridization on brush cytology in a Barrett esophagus surveillance population. Cancer, 109(10), 1980-1988. https://doi.org/10.1002/cncr.22643