Efficacy of limb cooling on the salvage of peripheral nerve from ischemic fiber degeneration

Mikihiro Kihara, James D. Schmelzer, Yoriko Kihara, Inge L. Smithson, Phillip Anson Low

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Since peripheral nerve has a large ischemic safety factor, hypothermia, by reducing metabolic demands, is potentially an efficacious technique to rescue nerve from ischemic fiber degeneration (IFD). We therefore evaluated the influence of temperature on the severity of IFD resulting from a standard ischemic stress. Ischemia to the left sciatic nerve in the rat was produced by embolization of 2 x 10 6 microspheres (14 μm) into its supplying arteries. The limb was embolized at three temperatures, 37°C, 32°C, and 28°C and was maintained at each temperature for an additional 4 h. End points, evaluated 7 days after embolization, for the embolized limb were: (1) behavioral scores, 0-11 in increasing limb function; (2) compound nerve action potential of sciatic-tibial nerve;(3) sciatic nerve blood flow (NBF, in mL/100 g/min); and (4) histologic grade, expressed as percentage of fibers undergoing IFD (0 = <5%; 1 = 5-25%; 2 = 26-50%; 3 = 51-75%; 4 = >75%). NBF was reduced in all groups, varying with temperature, and all indices of nerve structure and function were significantly improved with hypothermia. We conclude that hypothermia, easily achievable in a limb nerve, is highly efficacious in the rescue of nerve from IFD. These findings are of clinical relevance.

Original languageEnglish (US)
Pages (from-to)203-209
Number of pages7
JournalMuscle and Nerve
Volume19
Issue number2
DOIs
StatePublished - Feb 1996

Fingerprint

Peripheral Nerves
Nerve Fibers
Sciatic Nerve
Extremities
Hypothermia
Temperature
Tibial Nerve
Microspheres
Action Potentials
Ischemia
Arteries
Safety

Keywords

  • blood flow
  • hypothermia
  • ischemia
  • nerve
  • nerve conduction

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Efficacy of limb cooling on the salvage of peripheral nerve from ischemic fiber degeneration. / Kihara, Mikihiro; Schmelzer, James D.; Kihara, Yoriko; Smithson, Inge L.; Low, Phillip Anson.

In: Muscle and Nerve, Vol. 19, No. 2, 02.1996, p. 203-209.

Research output: Contribution to journalArticle

Kihara, Mikihiro ; Schmelzer, James D. ; Kihara, Yoriko ; Smithson, Inge L. ; Low, Phillip Anson. / Efficacy of limb cooling on the salvage of peripheral nerve from ischemic fiber degeneration. In: Muscle and Nerve. 1996 ; Vol. 19, No. 2. pp. 203-209.
@article{08bfb7fc91164f7b8e2fda56e28dd5f1,
title = "Efficacy of limb cooling on the salvage of peripheral nerve from ischemic fiber degeneration",
abstract = "Since peripheral nerve has a large ischemic safety factor, hypothermia, by reducing metabolic demands, is potentially an efficacious technique to rescue nerve from ischemic fiber degeneration (IFD). We therefore evaluated the influence of temperature on the severity of IFD resulting from a standard ischemic stress. Ischemia to the left sciatic nerve in the rat was produced by embolization of 2 x 10 6 microspheres (14 μm) into its supplying arteries. The limb was embolized at three temperatures, 37°C, 32°C, and 28°C and was maintained at each temperature for an additional 4 h. End points, evaluated 7 days after embolization, for the embolized limb were: (1) behavioral scores, 0-11 in increasing limb function; (2) compound nerve action potential of sciatic-tibial nerve;(3) sciatic nerve blood flow (NBF, in mL/100 g/min); and (4) histologic grade, expressed as percentage of fibers undergoing IFD (0 = <5{\%}; 1 = 5-25{\%}; 2 = 26-50{\%}; 3 = 51-75{\%}; 4 = >75{\%}). NBF was reduced in all groups, varying with temperature, and all indices of nerve structure and function were significantly improved with hypothermia. We conclude that hypothermia, easily achievable in a limb nerve, is highly efficacious in the rescue of nerve from IFD. These findings are of clinical relevance.",
keywords = "blood flow, hypothermia, ischemia, nerve, nerve conduction",
author = "Mikihiro Kihara and Schmelzer, {James D.} and Yoriko Kihara and Smithson, {Inge L.} and Low, {Phillip Anson}",
year = "1996",
month = "2",
doi = "10.1002/(SICI)1097-4598(199602)19:2<203::AID-MUS12>3.0.CO;2-V",
language = "English (US)",
volume = "19",
pages = "203--209",
journal = "Muscle and Nerve",
issn = "0148-639X",
publisher = "John Wiley and Sons Inc.",
number = "2",

}

TY - JOUR

T1 - Efficacy of limb cooling on the salvage of peripheral nerve from ischemic fiber degeneration

AU - Kihara, Mikihiro

AU - Schmelzer, James D.

AU - Kihara, Yoriko

AU - Smithson, Inge L.

AU - Low, Phillip Anson

PY - 1996/2

Y1 - 1996/2

N2 - Since peripheral nerve has a large ischemic safety factor, hypothermia, by reducing metabolic demands, is potentially an efficacious technique to rescue nerve from ischemic fiber degeneration (IFD). We therefore evaluated the influence of temperature on the severity of IFD resulting from a standard ischemic stress. Ischemia to the left sciatic nerve in the rat was produced by embolization of 2 x 10 6 microspheres (14 μm) into its supplying arteries. The limb was embolized at three temperatures, 37°C, 32°C, and 28°C and was maintained at each temperature for an additional 4 h. End points, evaluated 7 days after embolization, for the embolized limb were: (1) behavioral scores, 0-11 in increasing limb function; (2) compound nerve action potential of sciatic-tibial nerve;(3) sciatic nerve blood flow (NBF, in mL/100 g/min); and (4) histologic grade, expressed as percentage of fibers undergoing IFD (0 = <5%; 1 = 5-25%; 2 = 26-50%; 3 = 51-75%; 4 = >75%). NBF was reduced in all groups, varying with temperature, and all indices of nerve structure and function were significantly improved with hypothermia. We conclude that hypothermia, easily achievable in a limb nerve, is highly efficacious in the rescue of nerve from IFD. These findings are of clinical relevance.

AB - Since peripheral nerve has a large ischemic safety factor, hypothermia, by reducing metabolic demands, is potentially an efficacious technique to rescue nerve from ischemic fiber degeneration (IFD). We therefore evaluated the influence of temperature on the severity of IFD resulting from a standard ischemic stress. Ischemia to the left sciatic nerve in the rat was produced by embolization of 2 x 10 6 microspheres (14 μm) into its supplying arteries. The limb was embolized at three temperatures, 37°C, 32°C, and 28°C and was maintained at each temperature for an additional 4 h. End points, evaluated 7 days after embolization, for the embolized limb were: (1) behavioral scores, 0-11 in increasing limb function; (2) compound nerve action potential of sciatic-tibial nerve;(3) sciatic nerve blood flow (NBF, in mL/100 g/min); and (4) histologic grade, expressed as percentage of fibers undergoing IFD (0 = <5%; 1 = 5-25%; 2 = 26-50%; 3 = 51-75%; 4 = >75%). NBF was reduced in all groups, varying with temperature, and all indices of nerve structure and function were significantly improved with hypothermia. We conclude that hypothermia, easily achievable in a limb nerve, is highly efficacious in the rescue of nerve from IFD. These findings are of clinical relevance.

KW - blood flow

KW - hypothermia

KW - ischemia

KW - nerve

KW - nerve conduction

UR - http://www.scopus.com/inward/record.url?scp=0030071074&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030071074&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-4598(199602)19:2<203::AID-MUS12>3.0.CO;2-V

DO - 10.1002/(SICI)1097-4598(199602)19:2<203::AID-MUS12>3.0.CO;2-V

M3 - Article

VL - 19

SP - 203

EP - 209

JO - Muscle and Nerve

JF - Muscle and Nerve

SN - 0148-639X

IS - 2

ER -