Abstract
Conditioned medium derived from a rat central nervous system neuronal cell line B1O4 (B104 CM) was shown previously to contain uncharacterized potent mitogen(s) for oligodendrocyte/type-2 astrocyte (O-2A) progenitor cells. In this study, we demonstrated that B104 cells produce and secrete platelet-derived growth factor (PDGF)-AA homodimer, but not PDGF-B chain. B104 cells did not express other known potent mitogens for O-2A progenitor cells, including fibroblast growth factor-2 and neurotrophin-3. Unexpectedly, B104 cells also expressed transcripts of transforming growth factor-β1 (TGF- β1) and -β2 (TGF-β2), which are known to regulate O-2A progenitor cell differentiation and proliferation, and secreted exclusively the 25-kDa active forms of TGF-β1 and TGF-β2. Neutralization of B104 CM with anti-PDGF-AA antibody decreased proliferation of O-2A progenitor cells, whereas neutralization with anti-TGF-β antibodies had no effect. The combination of PDGF and TGF-β on proliferation was not equivalent to the effect of B104 CM, indicating the possibility of an unidentified growth factor. B104 CM maintained a high expression of PDGF-α receptor in oligodendrocytes. The observation that both a stimulatory factor (PDGF-AA) and a regulatory factor (TGF-β) for O-2A progenitor cell proliferation and differentiation are produced from a single neuronal cell line emphasizes the potential critical interaction between neurons and O-2A progenitor cells in myelination and possibly in remyelination.
Original language | English (US) |
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Pages (from-to) | 2281-2290 |
Number of pages | 10 |
Journal | Journal of neurochemistry |
Volume | 68 |
Issue number | 6 |
DOIs | |
State | Published - Jun 1997 |
Keywords
- Myelination
- Oligodendrocyte/type-2 astrocyte progenitor
- Platelet-derived growth factor
- Platelet-derived growth factor-α receptor
- Remyelination
- Transforming growth factor- β
ASJC Scopus subject areas
- Biochemistry
- Cellular and Molecular Neuroscience