Effects of the gag region on genome stability avian retroviral vectors that contain sequences from the bryan strain of rous sarcoma virus

Mark J Federspiel, S. H. Hughes

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We have previously described replication-competent Schmidt Ruppin-A Rous sarcoma virus (RSV)-based retroviral vectors that can be used to deliver and express genes in avian cells. We have continued to modify the prototype vectors to develop a more versatile and efficient system. Substitution of the polymerase (pol) region from the Bryan high-titer RSV (BH-RSV) for the SR-A RSV pol region of these retroviral vectors causes these viruses to replicate more efficiently. We cloned the gag regions from two independent BH-RSV-transformed cell lines and tested whether substituting either of these gag regions would improve the replication and/or gene expression of the vectors. Chimeric vectors were constructed in which the gag region of the prototype vector (SR-A RSV) was replaced with the corresponding segment of BH-RSV gag in vectors that had either the original SR-A RSV pol or the BH-RSV pol region. All vectors contained the bacterial chloramphenicol acetyltransferase gene (CAT). The results indicate that different SR-A RSV and BH-RSV gag-pol chimeras can significantly affect the level of viral and CAT gene expression. The insertion of one of the BH-RSV gag regions, but not the other, gave rise to viruses with unstable genomes.

Original languageEnglish (US)
Pages (from-to)211-220
Number of pages10
JournalVirology
Volume203
Issue number2
DOIs
StatePublished - 1994
Externally publishedYes

Fingerprint

Rous sarcoma virus
Genomic Instability
Chloramphenicol O-Acetyltransferase
Genes
Viruses
Gene Expression
Transformed Cell Line
Genome

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Effects of the gag region on genome stability avian retroviral vectors that contain sequences from the bryan strain of rous sarcoma virus. / Federspiel, Mark J; Hughes, S. H.

In: Virology, Vol. 203, No. 2, 1994, p. 211-220.

Research output: Contribution to journalArticle

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