Effects of the gag Region on Genome Stability: Avian Retroviral Vectors That Contain Sequences from the Bryan Strain of Rous Sarcoma Virus

Mark J. Federspiel, Stephen H. Hughes

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

We have previously described replication-competent Schmidt Ruppin-A Rous sarcoma virus (RSV)-based retroviral vectors that can be used to deliver and express genes in avian cells. We have continued to modify the prototype vectors to develop a more versatile and efficient system. Substitution of the polymerase (pol) region from the Bryan high-titer RSV (BH-RSV) for the SR-A RSV pol region of these retroviral vectors causes these viruses to replicate more efficiently. We cloned the gag regions from two independent SH-RSV-transformed cell lines and tested whether substituting either of these gag regions would improve the replication and/or gene expression of the vectors. Chimeric vectors were constructed in which the gag region of the prototype vector (SR-A RSV) was replaced with the corresponding segment of BH-RSV gag in vectors that had either the original SR-A RSV pol or the BH-RSV pol region. All vectors contained the bacterial chloramphenicol acetyltransferase gene (CAT). The results indicate that different SR-A RSV and BH-RSV gag-pol chimeras can significantly affect the level of vital and CAT gene expression. The insertion of one of the BH-RSV gag regions, but not the other, gave rise to viruses with unstable genomes.

Original languageEnglish (US)
Article number71478
Pages (from-to)211-220
Number of pages10
JournalVirology
Volume203
Issue number2
DOIs
StatePublished - Sep 1994

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ASJC Scopus subject areas

  • Virology

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