Effects of Telomerase Expression on Photodynamic Therapy of Barrett's Esophagus

Kenneth Ke Ning Wang, Marlys Anderson, Navtej Singh Buttar, Michel WongKeeSong, Lynn Borkenhagen, Lori Lutzke

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Photodynamic therapy has been applied to Barrett's esophagus and has been shown in prospective randomized studies to eliminate dysplasia as well as decrease the occurrence of cancer. However, the therapy is not always effective and there are issues with residual areas of Barrett's mucosa despite therapy. There has not been a good explanation for these residual areas and they seem to imply that there may exist a biological mechanism by which these cells may be resistant to photodynamic therapy. It was our aim to determine if known abnormalities in Barrett's mucosa could be correlated with the lack of response of some of these tissues. We examined the tissue from multiple patients who had response to therapy as well as those who did not respond. We assessed the tissue for p53 mutations, inactivation of p16, ploidy status, cell proliferation, telomerase activity, and degree of dysplasia. Interestingly, the only genetic marker than was found to be correlated with lack of response was p53 and telomerase activity. This suggests that cells that have lost mechanisms for cell death such as apoptosis or telomere shortening may be more resistant to photodynamic therapy. In this study, we examined patients before and after PDT for telomerase activity.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsD Kessel
Pages82-90
Number of pages9
Volume4952
DOIs
StatePublished - 2003
EventOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XII - San Jose, CA, United States
Duration: Jan 25 2003Jan 26 2003

Other

OtherOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XII
CountryUnited States
CitySan Jose, CA
Period1/25/031/26/03

Fingerprint

esophagus
Photodynamic therapy
therapy
Cell death
Tissue
Cell proliferation
telomeres
abnormalities
apoptosis
mutations
cells
death
deactivation
markers
cancer
occurrences
Mucous Membrane

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Wang, K. K. N., Anderson, M., Buttar, N. S., WongKeeSong, M., Borkenhagen, L., & Lutzke, L. (2003). Effects of Telomerase Expression on Photodynamic Therapy of Barrett's Esophagus. In D. Kessel (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4952, pp. 82-90) https://doi.org/10.1117/12.479434

Effects of Telomerase Expression on Photodynamic Therapy of Barrett's Esophagus. / Wang, Kenneth Ke Ning; Anderson, Marlys; Buttar, Navtej Singh; WongKeeSong, Michel; Borkenhagen, Lynn; Lutzke, Lori.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / D Kessel. Vol. 4952 2003. p. 82-90.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wang, KKN, Anderson, M, Buttar, NS, WongKeeSong, M, Borkenhagen, L & Lutzke, L 2003, Effects of Telomerase Expression on Photodynamic Therapy of Barrett's Esophagus. in D Kessel (ed.), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4952, pp. 82-90, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XII, San Jose, CA, United States, 1/25/03. https://doi.org/10.1117/12.479434
Wang KKN, Anderson M, Buttar NS, WongKeeSong M, Borkenhagen L, Lutzke L. Effects of Telomerase Expression on Photodynamic Therapy of Barrett's Esophagus. In Kessel D, editor, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 4952. 2003. p. 82-90 https://doi.org/10.1117/12.479434
Wang, Kenneth Ke Ning ; Anderson, Marlys ; Buttar, Navtej Singh ; WongKeeSong, Michel ; Borkenhagen, Lynn ; Lutzke, Lori. / Effects of Telomerase Expression on Photodynamic Therapy of Barrett's Esophagus. Proceedings of SPIE - The International Society for Optical Engineering. editor / D Kessel. Vol. 4952 2003. pp. 82-90
@inproceedings{7f86120a7a20440a9dcb370562a7dc13,
title = "Effects of Telomerase Expression on Photodynamic Therapy of Barrett's Esophagus",
abstract = "Photodynamic therapy has been applied to Barrett's esophagus and has been shown in prospective randomized studies to eliminate dysplasia as well as decrease the occurrence of cancer. However, the therapy is not always effective and there are issues with residual areas of Barrett's mucosa despite therapy. There has not been a good explanation for these residual areas and they seem to imply that there may exist a biological mechanism by which these cells may be resistant to photodynamic therapy. It was our aim to determine if known abnormalities in Barrett's mucosa could be correlated with the lack of response of some of these tissues. We examined the tissue from multiple patients who had response to therapy as well as those who did not respond. We assessed the tissue for p53 mutations, inactivation of p16, ploidy status, cell proliferation, telomerase activity, and degree of dysplasia. Interestingly, the only genetic marker than was found to be correlated with lack of response was p53 and telomerase activity. This suggests that cells that have lost mechanisms for cell death such as apoptosis or telomere shortening may be more resistant to photodynamic therapy. In this study, we examined patients before and after PDT for telomerase activity.",
author = "Wang, {Kenneth Ke Ning} and Marlys Anderson and Buttar, {Navtej Singh} and Michel WongKeeSong and Lynn Borkenhagen and Lori Lutzke",
year = "2003",
doi = "10.1117/12.479434",
language = "English (US)",
volume = "4952",
pages = "82--90",
editor = "D Kessel",
booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",

}

TY - GEN

T1 - Effects of Telomerase Expression on Photodynamic Therapy of Barrett's Esophagus

AU - Wang, Kenneth Ke Ning

AU - Anderson, Marlys

AU - Buttar, Navtej Singh

AU - WongKeeSong, Michel

AU - Borkenhagen, Lynn

AU - Lutzke, Lori

PY - 2003

Y1 - 2003

N2 - Photodynamic therapy has been applied to Barrett's esophagus and has been shown in prospective randomized studies to eliminate dysplasia as well as decrease the occurrence of cancer. However, the therapy is not always effective and there are issues with residual areas of Barrett's mucosa despite therapy. There has not been a good explanation for these residual areas and they seem to imply that there may exist a biological mechanism by which these cells may be resistant to photodynamic therapy. It was our aim to determine if known abnormalities in Barrett's mucosa could be correlated with the lack of response of some of these tissues. We examined the tissue from multiple patients who had response to therapy as well as those who did not respond. We assessed the tissue for p53 mutations, inactivation of p16, ploidy status, cell proliferation, telomerase activity, and degree of dysplasia. Interestingly, the only genetic marker than was found to be correlated with lack of response was p53 and telomerase activity. This suggests that cells that have lost mechanisms for cell death such as apoptosis or telomere shortening may be more resistant to photodynamic therapy. In this study, we examined patients before and after PDT for telomerase activity.

AB - Photodynamic therapy has been applied to Barrett's esophagus and has been shown in prospective randomized studies to eliminate dysplasia as well as decrease the occurrence of cancer. However, the therapy is not always effective and there are issues with residual areas of Barrett's mucosa despite therapy. There has not been a good explanation for these residual areas and they seem to imply that there may exist a biological mechanism by which these cells may be resistant to photodynamic therapy. It was our aim to determine if known abnormalities in Barrett's mucosa could be correlated with the lack of response of some of these tissues. We examined the tissue from multiple patients who had response to therapy as well as those who did not respond. We assessed the tissue for p53 mutations, inactivation of p16, ploidy status, cell proliferation, telomerase activity, and degree of dysplasia. Interestingly, the only genetic marker than was found to be correlated with lack of response was p53 and telomerase activity. This suggests that cells that have lost mechanisms for cell death such as apoptosis or telomere shortening may be more resistant to photodynamic therapy. In this study, we examined patients before and after PDT for telomerase activity.

UR - http://www.scopus.com/inward/record.url?scp=0141828969&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141828969&partnerID=8YFLogxK

U2 - 10.1117/12.479434

DO - 10.1117/12.479434

M3 - Conference contribution

VL - 4952

SP - 82

EP - 90

BT - Proceedings of SPIE - The International Society for Optical Engineering

A2 - Kessel, D

ER -