Effects of sex and ovariectomy on responses to platelets in porcine femoral veins

D. A. Lewis, M. P. Bracamonte, K. S. Rud, Virginia M Miller

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Estrogen replacement increases risk of venous thrombosis. In this study, we determined responses in vitro to platelets and platelet products in veins from adult male and intact and ovariectomized female pigs. When contracted with prostaglandin F2α, platelets (25,000 platelets/μl) caused relaxation in veins with endothelium. Higher numbers of platelets caused contraction in veins with and without endothelium. In veins without endothelium, contractions were greater in veins from male than in veins from female pigs, and contractions in intact female pig veins were greater than in ovariectomized females pig veins. Platelet products 5-hydroxytryptamine and thromboxane (analog U-46619) caused comparable contractions in all veins; contractions to prostacyclin were less in veins from intact female pigs. ADP caused comparable endothelium-dependent relaxations in all groups. These relaxations were increased by indomethacin in veins from intact males and females; with inhibition of nitric oxide, relaxations were comparable in all groups. These results suggest that venous responses to platelets vary with sex and presence of ovaries in female pigs. These variations reflect differences in type and quantity of substances released from platelets as well as the sensitivity of the smooth muscle to some vasoactive substances. In addition, products of cyclooxygenase may reduce endothelium-dependent relaxations in veins.

Original languageEnglish (US)
Pages (from-to)2823-2830
Number of pages8
JournalJournal of Applied Physiology
Volume91
Issue number6
StatePublished - 2001

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Femoral Vein
Ovariectomy
Veins
Swine
Blood Platelets
Endothelium
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
Estrogen Replacement Therapy
Dinoprost
Thromboxanes
Epoprostenol
Prostaglandin-Endoperoxide Synthases
Platelet Count
Venous Thrombosis
Indomethacin
Adenosine Diphosphate
Smooth Muscle
Ovary
Serotonin
Nitric Oxide

Keywords

  • Estrogen
  • Venous thrombosis

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Effects of sex and ovariectomy on responses to platelets in porcine femoral veins. / Lewis, D. A.; Bracamonte, M. P.; Rud, K. S.; Miller, Virginia M.

In: Journal of Applied Physiology, Vol. 91, No. 6, 2001, p. 2823-2830.

Research output: Contribution to journalArticle

Lewis, D. A. ; Bracamonte, M. P. ; Rud, K. S. ; Miller, Virginia M. / Effects of sex and ovariectomy on responses to platelets in porcine femoral veins. In: Journal of Applied Physiology. 2001 ; Vol. 91, No. 6. pp. 2823-2830.
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