Effects of retinoid ligands on RIP140: Molecular interaction with retinoid receptors and biological activity

Mariya Farooqui, Peter J. Franco, Jim Thompson, Hiroyuki Kagechika, Roshantha A S Chandraratna, Len Banaszak, Li Na Wei

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Receptor interacting protein 140 (RIP140) interacts with retinoic acid receptor (RAR) and retinoid X receptor (RXR) constitutively, but hormone binding enhances this interaction. The ligand-independent interaction is mediated by the amino and central regions of RIP140 which contain a total of nine copies of the LXXLL motif, whereas the agonist-induced interaction is mediated by its carboxyl terminus which contains a novel motif (1063-1076, LTKTNPILYYMLQK). The ligand-independent interaction could be enhanced slightly by agonists, whereas the ligand-dependent interaction was strictly agonist dependent for both RAR and RXR. In the context of heterodimers, ligand occupancy of RXR played a more dominant role for both molecular interaction and biological activity of RIP140. Competition and mutation studies demonstrated an essential role for 1067Asn and 1073Met for a ligand-dependent interaction. A model was proposed to address the constitutive and agonist-dependent interaction of RIP140 with RAR/RXR.

Original languageEnglish (US)
Pages (from-to)971-979
Number of pages9
JournalBiochemistry
Volume42
Issue number4
DOIs
StatePublished - Feb 4 2003
Externally publishedYes

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Nuclear Receptor Co-Repressor 1
Molecular interactions
Retinoids
Retinoid X Receptors
Bioactivity
Retinoic Acid Receptors
Ligands
Hormones
Mutation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Farooqui, M., Franco, P. J., Thompson, J., Kagechika, H., Chandraratna, R. A. S., Banaszak, L., & Wei, L. N. (2003). Effects of retinoid ligands on RIP140: Molecular interaction with retinoid receptors and biological activity. Biochemistry, 42(4), 971-979. https://doi.org/10.1021/bi020497k

Effects of retinoid ligands on RIP140 : Molecular interaction with retinoid receptors and biological activity. / Farooqui, Mariya; Franco, Peter J.; Thompson, Jim; Kagechika, Hiroyuki; Chandraratna, Roshantha A S; Banaszak, Len; Wei, Li Na.

In: Biochemistry, Vol. 42, No. 4, 04.02.2003, p. 971-979.

Research output: Contribution to journalArticle

Farooqui, M, Franco, PJ, Thompson, J, Kagechika, H, Chandraratna, RAS, Banaszak, L & Wei, LN 2003, 'Effects of retinoid ligands on RIP140: Molecular interaction with retinoid receptors and biological activity', Biochemistry, vol. 42, no. 4, pp. 971-979. https://doi.org/10.1021/bi020497k
Farooqui M, Franco PJ, Thompson J, Kagechika H, Chandraratna RAS, Banaszak L et al. Effects of retinoid ligands on RIP140: Molecular interaction with retinoid receptors and biological activity. Biochemistry. 2003 Feb 4;42(4):971-979. https://doi.org/10.1021/bi020497k
Farooqui, Mariya ; Franco, Peter J. ; Thompson, Jim ; Kagechika, Hiroyuki ; Chandraratna, Roshantha A S ; Banaszak, Len ; Wei, Li Na. / Effects of retinoid ligands on RIP140 : Molecular interaction with retinoid receptors and biological activity. In: Biochemistry. 2003 ; Vol. 42, No. 4. pp. 971-979.
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