Effects of prolonged hypoxia, reoxygenation, and shear stress on bovine aortic endothelial cell prostacyclin production

Hassan Al-Badawi, Hiram M. Soler, Michael T. Watkins

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Bovine aortic endothelial cell prostacyclin production was assessed under conditions of shear stress, hypoxia, and reoxygenation. Methods: Endothelial cell cultures (n = 8) grown on microcarrier beads were sequentially exposed to 2 h of normoxia (O2 = 20%), then 24 h of hypoxia (O2 = 2%) and 2 h of reoxygenation (O2 = 20%). Cell viability, levels of prostaglandin H2 synthase protein, and prostacyclin production were evaluated. Results: Prostacyclin production decreased significantly after 1 h of hypoxia, and became undetectable after 24 h. Reoxygenation did not result in recovery of normoxic levels of prostacyclin production. Immunoblots revealed no change in the levels of prostaglandin H2 synthase protein during hypoxia, and reoxygenation. Addition of arachidonic acid to cells (n = 5) during reoxygenation resulted in prompt recovery of prostacyclin synthesis. Conclusions: Endothelial cells exposed to sequential conditions of hypoxia and reoxygenation in the presence of shear stress decreased prostacyclin production. The decrease in prostacyclin production was not due to altered cell viability, levels of prostaglandin H2 synthase, or inactivation of prostaglandin H2 synthase.

Original languageEnglish (US)
Pages (from-to)127-139
Number of pages13
JournalProstaglandins and Other Lipid Mediators
Volume55
Issue number2-3
DOIs
StatePublished - Feb 1 1998

Keywords

  • Endothelial cells
  • Hypoxia
  • Prostacyclin
  • Reoxygenation
  • Shear stress

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Pharmacology
  • Cell Biology

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