Effects of Platycodon grandiflorum on lipopolysaccharide-stimulated production of prostaglandin E 2, nitric oxide, and interleukin-8 in mouse microglial BV2 cells

Mi Hyeon Jang, Chang Ju Kim, Ee Hwa Kim, Myung Gyou Kim, Kang Hyun Leem, Jeongseon Kim

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The roots of Platycodon grandiflorum, which belongs to the Campanulaceae family, have been used as a food material and as a traditional Oriental medicine. The effect of P. grandiflorum against lipopolysaccharide (LPS)-stimulated inflammation was investigated using 3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide assay, reverse transcription-polymerase chain reaction, prostaglandin E 2 (PGE 2) immunoassay, nitric oxide (NO) detection assay, and interleurkin-8 (IL-8) immunoassay on BV2 microglial cells. The aqueous extract of P. grandiflorum was shown to suppress PGE 2 synthesis and NO production by inhibiting LPS-stimulated cyclooxygenase (COX)-2 activity and expression of inducible NO synthase (iNOS) mRNAs. In addition, the treatment with P. grandiflorum reduced the LPS-induced IL-8 release. These results suggest that P. grandiflorum inhibits PGE 2 and NO production through its suppression of LPS-induced COX-2 and iNOS expression, and also reduces IL-8 secretion by microglial cells.

Original languageEnglish (US)
Pages (from-to)169-174
Number of pages6
JournalJournal of Medicinal Food
Volume9
Issue number2
DOIs
StatePublished - Jun 1 2006

Keywords

  • Interleukin-8
  • Microglia
  • Nitric oxide
  • Platycodon grandiflorum
  • Prostaglandin E

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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