Abstract
The roots of Platycodon grandiflorum, which belongs to the Campanulaceae family, have been used as a food material and as a traditional Oriental medicine. The effect of P. grandiflorum against lipopolysaccharide (LPS)-stimulated inflammation was investigated using 3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide assay, reverse transcription-polymerase chain reaction, prostaglandin E 2 (PGE 2) immunoassay, nitric oxide (NO) detection assay, and interleurkin-8 (IL-8) immunoassay on BV2 microglial cells. The aqueous extract of P. grandiflorum was shown to suppress PGE 2 synthesis and NO production by inhibiting LPS-stimulated cyclooxygenase (COX)-2 activity and expression of inducible NO synthase (iNOS) mRNAs. In addition, the treatment with P. grandiflorum reduced the LPS-induced IL-8 release. These results suggest that P. grandiflorum inhibits PGE 2 and NO production through its suppression of LPS-induced COX-2 and iNOS expression, and also reduces IL-8 secretion by microglial cells.
Original language | English (US) |
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Pages (from-to) | 169-174 |
Number of pages | 6 |
Journal | Journal of Medicinal Food |
Volume | 9 |
Issue number | 2 |
DOIs | |
State | Published - Jun 2006 |
Keywords
- Interleukin-8
- Microglia
- Nitric oxide
- Platycodon grandiflorum
- Prostaglandin E
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Nutrition and Dietetics