Effects of phenylacetate on cells from patients with b-chronic lymphocytic leukemia

Cdr T G Call, M. J. Stenson, Thomas Elmer Witzig

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Peripheral blood mononuclear cells from 11 patients with untreated B-chronic lymphocytic leukemia (CLL) were exposed to sodium phenylacetate (NaPA) in culture to assess its ability to induce differentiation. We found no evidence of cellular differentiation or induction of tartrate resistant acid phosphatase activity, as seen when B-CLL cells were treated with phorbol ester. We observed a striking decrease in the viability of the B-CLL cells in a time and dose dependent fashion when exposed to NaPA. After six days of culture, control cells from the 11 patients studied had a median viability of 90% whereas cells exposed to NaPA at 5 and 10 mM concentrations had median viabilities of 39 and 16% respectively. The cells treated with NaPA developed prominent cytoplasmic vacuoles. NaPA binds and depletes glutamine which is an important amino acid for lymphocyte metabolism. Although the mechanism of the cytocidal effects demonstrated in this study are unknown, they may relate at least partially to glutamine deprivation.

Original languageEnglish (US)
Pages (from-to)145-149
Number of pages5
JournalLeukemia and Lymphoma
Volume14
Issue number1-2
DOIs
StatePublished - 1994

Fingerprint

B-Cell Chronic Lymphocytic Leukemia
Glutamine
Phorbol Esters
Vacuoles
Blood Cells
Cell Culture Techniques
Lymphocytes
Amino Acids
phenylacetic acid

Keywords

  • Chronic lymphocytic leukemia
  • Cytotoxicity
  • Differentiation
  • Glutamine
  • Phenylacetate

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Effects of phenylacetate on cells from patients with b-chronic lymphocytic leukemia. / Call, Cdr T G; Stenson, M. J.; Witzig, Thomas Elmer.

In: Leukemia and Lymphoma, Vol. 14, No. 1-2, 1994, p. 145-149.

Research output: Contribution to journalArticle

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