Effects of multiple injections of hypertonic dextrose in the rabbit carpal tunnel: A potential model of carpal tunnel syndrome development

Yuichi Yoshii, Chunfeng D Zhao, James D. Schmelzer, Phillip Anson Low, Kai Nan An, Peter C Amadio

Research output: Contribution to journalArticle

14 Scopus citations


Background: This study investigated the effects of a series of four hypertonic dextrose injections on the subsynovial connective tissue (SSCT) and median nerve within the carpal tunnel of a rabbit model. Methods: Twenty New Zealand white rabbits were used. One forepaw carpal tunnel was randomly injected with 0.1 ml of 10 % dextrose solution. The contralateral forepaw was injected with a similar amount of saline. This injection was made once per week for 4 weeks. The animals were killed at 16 weeks after the initial injection and were evaluated by electrophysiology (EP), SSCT mechanical testing, and histology. Results: Mechanical testing revealed significantly greater ultimate load and energy absorption in the dextrose injection group compared to the saline injection group (P < 0.05). Histological evaluation revealed SSCT fibrosis and thickening and edema in the median nerve bundles in the dextrose injection group. There was a prolongation in the latency of the EP test in the dextrose injection group (P = 0.08). Conclusions: Previous studies had shown that one or two injections of 10 % dextrose could induce moderate SSCT fibrosis and mild EP changes without nerve histology changes. In this study, we have shown that higher doses create more severe fibrosis and, most importantly, more severe neuropathy, suggesting a dose-response effect, and confirming this as a potentially useful animal model for researching the etiology and treatment of carpal tunnel syndrome.

Original languageEnglish (US)
Pages (from-to)52-57
Number of pages6
Issue number1
StatePublished - 2014



  • Carpal tunnel syndrome
  • Hypertonic dextrose
  • Rabbit
  • Subsynovial connective tissue

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Surgery

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