Effects of meal ingestion on intramyocellular ceramide concentrations and fractional de novo synthesis in humans

Jin Ook Chung, Christina Koutsari, Agnieszka Urszula Blachnio-Zablieska, Kazanna C. Hames, Michael Dennis Jensen

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We investigated the effects of meal ingestion on intramyofibrillar (IMF) and subsarcolemmal (SS) ceramide metabolism in volunteers ranging from lean to obese. Thirty-eight women and men underwent a steady-state meal ingestion protocol that included a 6.5-h infusion of [U-13C]palmitate and muscle biopsies 1.5 and 6.5 h after starting the tracer infusion. We measured IMF and SS sphingolipid concentrations and the contribution of plasma palmitate to intramyocellular C16:0 ceramide by use of LC-MS-MS. In response to meal ingestion SS C24 ceramide concentrations, but not C14-C20 concentrations, increased significantly. IMF ceramide concentrations did not change. The increases in SS C24 ceramides were negatively related to parameters of insulin resistance. The fractional contribution of plasma palmitate to intramyocellular C16:0 ceramides in both IMF and SS fractions was inversely related to overweight status (β = -0.432, P = 0.0095 and β = -0.443, P = 0.0058, respectively). These data indicate that meal ingestion has differing effects on SS ceramide subspecies and suggest that the fractional de novo synthesis of intramyocellular ceramide from plasma palmitate in the postprandial condition is reduced in those who are overweight.

Original languageEnglish (US)
Pages (from-to)E105-E114
JournalAmerican journal of physiology. Endocrinology and metabolism
Volume314
Issue number2
DOIs
StatePublished - Feb 1 2018

Fingerprint

Ceramides
Meals
Eating
Palmitates
Sphingolipids
Insulin Resistance
Volunteers
Biopsy
Muscles

Keywords

  • insulin resistance
  • isotopic tracers
  • meals
  • obesity
  • skeletal muscle

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Effects of meal ingestion on intramyocellular ceramide concentrations and fractional de novo synthesis in humans. / Chung, Jin Ook; Koutsari, Christina; Blachnio-Zablieska, Agnieszka Urszula; Hames, Kazanna C.; Jensen, Michael Dennis.

In: American journal of physiology. Endocrinology and metabolism, Vol. 314, No. 2, 01.02.2018, p. E105-E114.

Research output: Contribution to journalArticle

Chung, Jin Ook ; Koutsari, Christina ; Blachnio-Zablieska, Agnieszka Urszula ; Hames, Kazanna C. ; Jensen, Michael Dennis. / Effects of meal ingestion on intramyocellular ceramide concentrations and fractional de novo synthesis in humans. In: American journal of physiology. Endocrinology and metabolism. 2018 ; Vol. 314, No. 2. pp. E105-E114.
@article{291ee519470749b8bf5c006f0404f716,
title = "Effects of meal ingestion on intramyocellular ceramide concentrations and fractional de novo synthesis in humans",
abstract = "We investigated the effects of meal ingestion on intramyofibrillar (IMF) and subsarcolemmal (SS) ceramide metabolism in volunteers ranging from lean to obese. Thirty-eight women and men underwent a steady-state meal ingestion protocol that included a 6.5-h infusion of [U-13C]palmitate and muscle biopsies 1.5 and 6.5 h after starting the tracer infusion. We measured IMF and SS sphingolipid concentrations and the contribution of plasma palmitate to intramyocellular C16:0 ceramide by use of LC-MS-MS. In response to meal ingestion SS C24 ceramide concentrations, but not C14-C20 concentrations, increased significantly. IMF ceramide concentrations did not change. The increases in SS C24 ceramides were negatively related to parameters of insulin resistance. The fractional contribution of plasma palmitate to intramyocellular C16:0 ceramides in both IMF and SS fractions was inversely related to overweight status (β = -0.432, P = 0.0095 and β = -0.443, P = 0.0058, respectively). These data indicate that meal ingestion has differing effects on SS ceramide subspecies and suggest that the fractional de novo synthesis of intramyocellular ceramide from plasma palmitate in the postprandial condition is reduced in those who are overweight.",
keywords = "insulin resistance, isotopic tracers, meals, obesity, skeletal muscle",
author = "Chung, {Jin Ook} and Christina Koutsari and Blachnio-Zablieska, {Agnieszka Urszula} and Hames, {Kazanna C.} and Jensen, {Michael Dennis}",
year = "2018",
month = "2",
day = "1",
doi = "10.1152/ajpendo.00153.2017",
language = "English (US)",
volume = "314",
pages = "E105--E114",
journal = "American Journal of Physiology",
issn = "0193-1849",
publisher = "American Physiological Society",
number = "2",

}

TY - JOUR

T1 - Effects of meal ingestion on intramyocellular ceramide concentrations and fractional de novo synthesis in humans

AU - Chung, Jin Ook

AU - Koutsari, Christina

AU - Blachnio-Zablieska, Agnieszka Urszula

AU - Hames, Kazanna C.

AU - Jensen, Michael Dennis

PY - 2018/2/1

Y1 - 2018/2/1

N2 - We investigated the effects of meal ingestion on intramyofibrillar (IMF) and subsarcolemmal (SS) ceramide metabolism in volunteers ranging from lean to obese. Thirty-eight women and men underwent a steady-state meal ingestion protocol that included a 6.5-h infusion of [U-13C]palmitate and muscle biopsies 1.5 and 6.5 h after starting the tracer infusion. We measured IMF and SS sphingolipid concentrations and the contribution of plasma palmitate to intramyocellular C16:0 ceramide by use of LC-MS-MS. In response to meal ingestion SS C24 ceramide concentrations, but not C14-C20 concentrations, increased significantly. IMF ceramide concentrations did not change. The increases in SS C24 ceramides were negatively related to parameters of insulin resistance. The fractional contribution of plasma palmitate to intramyocellular C16:0 ceramides in both IMF and SS fractions was inversely related to overweight status (β = -0.432, P = 0.0095 and β = -0.443, P = 0.0058, respectively). These data indicate that meal ingestion has differing effects on SS ceramide subspecies and suggest that the fractional de novo synthesis of intramyocellular ceramide from plasma palmitate in the postprandial condition is reduced in those who are overweight.

AB - We investigated the effects of meal ingestion on intramyofibrillar (IMF) and subsarcolemmal (SS) ceramide metabolism in volunteers ranging from lean to obese. Thirty-eight women and men underwent a steady-state meal ingestion protocol that included a 6.5-h infusion of [U-13C]palmitate and muscle biopsies 1.5 and 6.5 h after starting the tracer infusion. We measured IMF and SS sphingolipid concentrations and the contribution of plasma palmitate to intramyocellular C16:0 ceramide by use of LC-MS-MS. In response to meal ingestion SS C24 ceramide concentrations, but not C14-C20 concentrations, increased significantly. IMF ceramide concentrations did not change. The increases in SS C24 ceramides were negatively related to parameters of insulin resistance. The fractional contribution of plasma palmitate to intramyocellular C16:0 ceramides in both IMF and SS fractions was inversely related to overweight status (β = -0.432, P = 0.0095 and β = -0.443, P = 0.0058, respectively). These data indicate that meal ingestion has differing effects on SS ceramide subspecies and suggest that the fractional de novo synthesis of intramyocellular ceramide from plasma palmitate in the postprandial condition is reduced in those who are overweight.

KW - insulin resistance

KW - isotopic tracers

KW - meals

KW - obesity

KW - skeletal muscle

UR - http://www.scopus.com/inward/record.url?scp=85055907884&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055907884&partnerID=8YFLogxK

U2 - 10.1152/ajpendo.00153.2017

DO - 10.1152/ajpendo.00153.2017

M3 - Article

C2 - 28970356

AN - SCOPUS:85055907884

VL - 314

SP - E105-E114

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0193-1849

IS - 2

ER -