Effects of lung preservation with Euro-Collins and University of Wisconsin solutions on endothelium-dependent relaxations

Martin Strüber, Klaus A. Ehlers, Folke N. Nilsson, Virginia M Miller, Christopher G A McGregor, Axel Haverich

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background. This study compares the effect of lung preservation using flush perfusion of Euro-Collins or University of Wisconsin solution on the pulmonary vascular function of endothelium-dependent and endothelium- independent relaxations. Methods. Rings of canine intrapulmonary arteries were studied after 6 hours of cold ischemia in Euro-Collins or University of Wisconsin preservation solution. Endothelium-dependent and endothelium- independent relaxations were induced in organ chamber experiments. To also study pulmonary resistance vessels, endothelium-dependent relaxations were induced in in vitro perfused intact rabbit lungs. Results. In the organ chamber experiments, a moderate but significant (p < 0.05) reduction in endothelium dependent relaxations were found in the perfused and stored vessels. In perfused rabbit lungs, a decrease in the endothelial response occurred immediately after perfusion with Euro-Collins solution. However, a recovery and overshooting response was found after preservation with either solution and 6 hours of cold ischemia. A significant increase in the sensitivity of smooth muscle cells to nitric oxide was shown in both preparations. Conclusions. Both crystalloid perfusion fluids cause a decrease in endothelial function during the perfusion procedure. In contrast, endothelial function is well preserved during the ischemic time. University of Wisconsin solution induced a higher sensitivity of the vascular smooth muscle to the endothelium-derived relaxing factor nitric oxide. A reduction in pulmonary vascular resistance after University of Wisconsin preservation may be of importance in subsequent clinical lung transplantation.

Original languageEnglish (US)
Pages (from-to)1428-1435
Number of pages8
JournalAnnals of Thoracic Surgery
Volume63
Issue number5
DOIs
StatePublished - May 1997

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Endothelium
Lung
Perfusion
Cold Ischemia
Nitric Oxide
Rabbits
Endothelium-Dependent Relaxing Factors
Lung Transplantation
Vascular Endothelium
Vascular Smooth Muscle
Vascular Resistance
Smooth Muscle Myocytes
Canidae
Arteries

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

Effects of lung preservation with Euro-Collins and University of Wisconsin solutions on endothelium-dependent relaxations. / Strüber, Martin; Ehlers, Klaus A.; Nilsson, Folke N.; Miller, Virginia M; McGregor, Christopher G A; Haverich, Axel.

In: Annals of Thoracic Surgery, Vol. 63, No. 5, 05.1997, p. 1428-1435.

Research output: Contribution to journalArticle

Strüber, Martin ; Ehlers, Klaus A. ; Nilsson, Folke N. ; Miller, Virginia M ; McGregor, Christopher G A ; Haverich, Axel. / Effects of lung preservation with Euro-Collins and University of Wisconsin solutions on endothelium-dependent relaxations. In: Annals of Thoracic Surgery. 1997 ; Vol. 63, No. 5. pp. 1428-1435.
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abstract = "Background. This study compares the effect of lung preservation using flush perfusion of Euro-Collins or University of Wisconsin solution on the pulmonary vascular function of endothelium-dependent and endothelium- independent relaxations. Methods. Rings of canine intrapulmonary arteries were studied after 6 hours of cold ischemia in Euro-Collins or University of Wisconsin preservation solution. Endothelium-dependent and endothelium- independent relaxations were induced in organ chamber experiments. To also study pulmonary resistance vessels, endothelium-dependent relaxations were induced in in vitro perfused intact rabbit lungs. Results. In the organ chamber experiments, a moderate but significant (p < 0.05) reduction in endothelium dependent relaxations were found in the perfused and stored vessels. In perfused rabbit lungs, a decrease in the endothelial response occurred immediately after perfusion with Euro-Collins solution. However, a recovery and overshooting response was found after preservation with either solution and 6 hours of cold ischemia. A significant increase in the sensitivity of smooth muscle cells to nitric oxide was shown in both preparations. Conclusions. Both crystalloid perfusion fluids cause a decrease in endothelial function during the perfusion procedure. In contrast, endothelial function is well preserved during the ischemic time. University of Wisconsin solution induced a higher sensitivity of the vascular smooth muscle to the endothelium-derived relaxing factor nitric oxide. A reduction in pulmonary vascular resistance after University of Wisconsin preservation may be of importance in subsequent clinical lung transplantation.",
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