Effects of joint contracture on the contralateral unoperated limb in a rabbit knee contracture model: A biomechanical and genetic study

Matthew P. Abdel, Mark E. Morrey, Diane E. Grill, Christopher P. Kolbert, Kai Nan An, Scott P. Steinmann, Joaquin Sanchez-Sotelo, Bernard F. Morrey

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

In most animal models, unoperated contralateral limbs are used as controls. However, in some experimental circumstances, the contralateral limb may represent a skewed control. The main purpose of this study was to determine if the unoperated contralateral limb could be used as a control, or if a different unoperated animal's limb should be used instead. Seventeen rabbits were divided into two groups. Group 1 rabbits (n = 12) underwent surgery on their right limbs to induce a contracture. Group 2 rabbits (n = 5) underwent no surgery. The left non-operated limbs of rabbits in group 1 were biomechanically and genetically compared to the limbs of unoperated rabbits in group 2 with the use of a validated joint measuring device and custom microarray, respectively. After 8 weeks of immobilization, there was a statistically greater flexion contracture in the unoperated contralateral limbs compared to the limbs of animals that received no surgery(8.4 ± 8.9° vs. 0 ± 0°; p-value = 0.03). When animals were remobilized for an additional 16 weeks, the significance between groups was lost (11.9 ± 21.4° vs. 8.9 ± 9.5°; p = 0.38). Similarly, there was a statistically significant increase in nine genes at 8 weeks (p < 0.001). However, at 24 weeks, only the PMCA 1 gene was statically increased (p < 0.001). In our rabbit model, the non-operated limb develops a small flexion contracture at 8 weeks. After 16 weeks of remobilization, there is no biomechanical or genetic difference between contralateral non-operated limbs and limbs of animals not undergoing any surgical intervention. Given the biomechanical and genetic findings, the contralateral non-operated limb can be used as a valid control. ©

Original languageEnglish (US)
Pages (from-to)1581-1585
Number of pages5
JournalJournal of Orthopaedic Research
Volume30
Issue number10
DOIs
StatePublished - Oct 1 2012

Keywords

  • biomechanics
  • contralateral limbs
  • genetic variation
  • joint contractures
  • rabbit model

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

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    Abdel, M. P., Morrey, M. E., Grill, D. E., Kolbert, C. P., An, K. N., Steinmann, S. P., Sanchez-Sotelo, J., & Morrey, B. F. (2012). Effects of joint contracture on the contralateral unoperated limb in a rabbit knee contracture model: A biomechanical and genetic study. Journal of Orthopaedic Research, 30(10), 1581-1585. https://doi.org/10.1002/jor.22106