Effects of immunological sympathectomy on postnasal peptide expression in the rat adrenal medulla

Hans Holgert, Hugo Lagercrantz, Åke Dagerlind, Boyd K. Hartman, Costatino Cozzari, William Stephen Brimijoin, Tomas Hökfelt

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Administration of monoclonal antibodies against acetylcholinesterase (AChE-mabs) to adult rats leads to a selective degeneration of the acetylcholine esterase- (AChE), choline acetyltransferase- (ChAT) and enkephalin- (ENK) positive preganglionic fibres of the splanchnic nerve innervating the adrenal gland. Here we used this approach of immunological sympathectomy, performed at postnatal day 2 (P2), in an attempt to study the developmental role of the preganglionic fibres in the adrenal medulla in more detail. Analysis was performed at P16 and revealed that the effect of this treatment varied considerably between animals, as judged by the number of remaining AChE-, ChAT- and ENK-positive fibres. The number and intensity especially of ENK fibres in the adrenal medulla correlated negatively with the number and staining intensity of ENK-immunoreactive chromaffin cells, suggesting a 'dose-response' relationship. Thus, the high early postnatal levels of ENK-like immunoreactivity generally persisted in chromaffin cells of adrenals with a successful immunosympathectomy, i.e. in those adrenals that lacked AChE-, ChAT- and ENK-positive nerves. In contrast, calcitonin gene-related peptide-like immunoreactivity in nerves and chromaffin cells was not affected. Large and strongly AChE-positive intra-adrenal ganglion neurones, recently termed type I ganglion neurones, were present also after AChE-mab treatment and had an apparently normal morphology. These results indicate a role for preganglionic fibres in the developmental regulation of ENK in the chromaffin cells. However, these fibres appear less important for the postnatal development of the type I ganglion neurones.

Original languageEnglish (US)
Pages (from-to)88-95
Number of pages8
JournalDevelopmental Brain Research
Volume97
Issue number1
DOIs
StatePublished - Nov 22 1996

Fingerprint

Adrenal Medulla
Sympathectomy
Enkephalins
Esterases
Acetylcholine
Chromaffin Cells
Peptides
Choline O-Acetyltransferase
Ganglia
Neurons
Splanchnic Nerves
Calcitonin Gene-Related Peptide
Acetylcholinesterase
Adrenal Glands
Monoclonal Antibodies
Staining and Labeling

Keywords

  • Acetylcholinesterase
  • Calcitonin gene-related peptide
  • Choline acetyltransferase
  • Enkephalin
  • Galanin
  • Galanin message-associated peptide
  • Rat

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Effects of immunological sympathectomy on postnasal peptide expression in the rat adrenal medulla. / Holgert, Hans; Lagercrantz, Hugo; Dagerlind, Åke; Hartman, Boyd K.; Cozzari, Costatino; Brimijoin, William Stephen; Hökfelt, Tomas.

In: Developmental Brain Research, Vol. 97, No. 1, 22.11.1996, p. 88-95.

Research output: Contribution to journalArticle

Holgert, Hans ; Lagercrantz, Hugo ; Dagerlind, Åke ; Hartman, Boyd K. ; Cozzari, Costatino ; Brimijoin, William Stephen ; Hökfelt, Tomas. / Effects of immunological sympathectomy on postnasal peptide expression in the rat adrenal medulla. In: Developmental Brain Research. 1996 ; Vol. 97, No. 1. pp. 88-95.
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abstract = "Administration of monoclonal antibodies against acetylcholinesterase (AChE-mabs) to adult rats leads to a selective degeneration of the acetylcholine esterase- (AChE), choline acetyltransferase- (ChAT) and enkephalin- (ENK) positive preganglionic fibres of the splanchnic nerve innervating the adrenal gland. Here we used this approach of immunological sympathectomy, performed at postnatal day 2 (P2), in an attempt to study the developmental role of the preganglionic fibres in the adrenal medulla in more detail. Analysis was performed at P16 and revealed that the effect of this treatment varied considerably between animals, as judged by the number of remaining AChE-, ChAT- and ENK-positive fibres. The number and intensity especially of ENK fibres in the adrenal medulla correlated negatively with the number and staining intensity of ENK-immunoreactive chromaffin cells, suggesting a 'dose-response' relationship. Thus, the high early postnatal levels of ENK-like immunoreactivity generally persisted in chromaffin cells of adrenals with a successful immunosympathectomy, i.e. in those adrenals that lacked AChE-, ChAT- and ENK-positive nerves. In contrast, calcitonin gene-related peptide-like immunoreactivity in nerves and chromaffin cells was not affected. Large and strongly AChE-positive intra-adrenal ganglion neurones, recently termed type I ganglion neurones, were present also after AChE-mab treatment and had an apparently normal morphology. These results indicate a role for preganglionic fibres in the developmental regulation of ENK in the chromaffin cells. However, these fibres appear less important for the postnatal development of the type I ganglion neurones.",
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AU - Dagerlind, Åke

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AU - Cozzari, Costatino

AU - Brimijoin, William Stephen

AU - Hökfelt, Tomas

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