Effects of human class I transgenes on Toxoplasma gondii cyst formation

Charles R. Brown, Chella S. David, San Jay Khare, Rima McLeod

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

MHC class I genes are important in the regulation of cyst number in mice perorally infected with Toxoplasma gondii. Since human MHC class I molecules may be capable of functioning as restriction elements in mice, to determine whether class I genes also control outcome of T. gondii infection for humans, a transgenic mouse model was developed using human class I genes in mice. Human HLA-B27 and Cw3 transgenic mice, with the cyst-susceptible B10 mouse background, were tested for resistance to cyst formation after peroral infection with the Me49 strain of T. gondii. Introduction of the B27 gene into susceptible mice made them even more susceptible to T. gondii brain cyst formation. In contrast, HLA-DB27 transgenic mice that have a substitution of the α3 portion of the human class I molecule with the α3 portion of the mouse K(d) molecule had the same number of brain cysts as susceptible B10 control mice when perorally infected. Similarly, HLA-Cw3 transgenic mice had the same number of brain cysts as the susceptible B10 controls. These results indicate that the interaction between CD8 on mouse T cells and the human class I molecule in transgenic mice does not always occur, and sometimes may hinder a normal response. Furthermore, B27 and Cw3 transgenes did not generate resistance to cyst formation in infection by T. gondii.

Original languageEnglish (US)
Pages (from-to)4537-4541
Number of pages5
JournalJournal of Immunology
Volume152
Issue number9
StatePublished - May 1 1994

Fingerprint

Toxoplasma
Transgenes
Cysts
Transgenic Mice
MHC Class I Genes
Toxoplasmosis
Brain
HLA-B27 Antigen
T-Lymphocytes
Infection
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Brown, C. R., David, C. S., Khare, S. J., & McLeod, R. (1994). Effects of human class I transgenes on Toxoplasma gondii cyst formation. Journal of Immunology, 152(9), 4537-4541.

Effects of human class I transgenes on Toxoplasma gondii cyst formation. / Brown, Charles R.; David, Chella S.; Khare, San Jay; McLeod, Rima.

In: Journal of Immunology, Vol. 152, No. 9, 01.05.1994, p. 4537-4541.

Research output: Contribution to journalArticle

Brown, CR, David, CS, Khare, SJ & McLeod, R 1994, 'Effects of human class I transgenes on Toxoplasma gondii cyst formation', Journal of Immunology, vol. 152, no. 9, pp. 4537-4541.
Brown CR, David CS, Khare SJ, McLeod R. Effects of human class I transgenes on Toxoplasma gondii cyst formation. Journal of Immunology. 1994 May 1;152(9):4537-4541.
Brown, Charles R. ; David, Chella S. ; Khare, San Jay ; McLeod, Rima. / Effects of human class I transgenes on Toxoplasma gondii cyst formation. In: Journal of Immunology. 1994 ; Vol. 152, No. 9. pp. 4537-4541.
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