Effects of HIV-1 and herpes simplex virus type 2 infection on lymphocyte and dendritic cell density in adult foreskins from Rakai, Uganda

Kristine E. Johnson, Andrew D. Redd, Thomas C. Quinn, Aleisha N. Collinson-Streng, Toby Cornish, Xiangrong Kong, Rajni Sharma, Aaron A R Tobian, Benjamin Tsai, Mark E. Sherman, Godfrey Kigozi, David Serwadda, Maria J. Wawer, Ronald H. Gray

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Abstract

Background. Male circumcision reduces human immunodeficiency virus (HIV) and herpes simplex virus type 2 (HSV-2) acquisition, and HSV-2 infection is associated with an increased risk of HIV acquisition. To assess the cellular basis for these associations, we estimated immunologic cellular densities in foreskin tissue. Methods. Immunostained CD1a+ dendritic cell and CD4+ and CD8+ T cell densities were quantified in foreskin samples obtained from medical circumcision in Rakai, Uganda (35 HIV-infected, HSV-2-infected men; 5 HIV-infected, HSV-2-uninfected men; 22 HIV-uninfected, HSV-2-infected men; and 29 HIV-uninfected, HSV-2-uninfected men. Results. CD1A+ dendritic cell densities did not vary by HIV or HSV-2 status. Compared with densities in HIV-uninfected, HSV-2-uninfected men (mean, 26.8 cells/mm2), CD4+ T cell densities were similar in the HIV-infected, HSV-2-infected group (mean, 28.7 cells/mm2), were significantly decreased in the HIV-infected, HSV-2-uninfected group (mean, 11.2; P < .05), and were increased in the HIV-uninfected, HSV-2-infected group (mean, 68.7; P < .05). Dermal CD8+ T cell densities were higher in the HIV and HSV-2-coinfected group (mean, 102.9) than in the HIV-uninfected, HSV-2-uninfected group (mean, 10.0; P < .001), the HIV-infected, HSV-2-uninfected group (mean, 27.3; P < .001), and the HIV-uninfected, HSV-2-infected group (mean, 25.3; P < .005). Discussion. The increased CD4+ cellular density in the HIV-uninfected, HSV-2-infected men may help to explain why HSV-2-infected men are at increased risk of HIV acquisition. The absence of this increase in men coinfected with both HIV and HSV-2 is likely in part the result of the progressive loss of CD4+ cells in HIV infection. Conversely, HIV and HSV-2 coinfection appears to synergistically increase CD8+ T cell densities.

Original languageEnglish (US)
Pages (from-to)602-609
Number of pages8
JournalJournal of Infectious Diseases
Volume203
Issue number5
DOIs
StatePublished - Mar 1 2011

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Foreskin
Human Herpesvirus 2
Uganda
Virus Diseases
Dendritic Cells
HIV-1
Cell Count
HIV
Lymphocytes
T-Lymphocytes

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

Cite this

Johnson, K. E., Redd, A. D., Quinn, T. C., Collinson-Streng, A. N., Cornish, T., Kong, X., ... Gray, R. H. (2011). Effects of HIV-1 and herpes simplex virus type 2 infection on lymphocyte and dendritic cell density in adult foreskins from Rakai, Uganda. Journal of Infectious Diseases, 203(5), 602-609. https://doi.org/10.1093/infdis/jiq091

Effects of HIV-1 and herpes simplex virus type 2 infection on lymphocyte and dendritic cell density in adult foreskins from Rakai, Uganda. / Johnson, Kristine E.; Redd, Andrew D.; Quinn, Thomas C.; Collinson-Streng, Aleisha N.; Cornish, Toby; Kong, Xiangrong; Sharma, Rajni; Tobian, Aaron A R; Tsai, Benjamin; Sherman, Mark E.; Kigozi, Godfrey; Serwadda, David; Wawer, Maria J.; Gray, Ronald H.

In: Journal of Infectious Diseases, Vol. 203, No. 5, 01.03.2011, p. 602-609.

Research output: Contribution to journalArticle

Johnson, KE, Redd, AD, Quinn, TC, Collinson-Streng, AN, Cornish, T, Kong, X, Sharma, R, Tobian, AAR, Tsai, B, Sherman, ME, Kigozi, G, Serwadda, D, Wawer, MJ & Gray, RH 2011, 'Effects of HIV-1 and herpes simplex virus type 2 infection on lymphocyte and dendritic cell density in adult foreskins from Rakai, Uganda', Journal of Infectious Diseases, vol. 203, no. 5, pp. 602-609. https://doi.org/10.1093/infdis/jiq091
Johnson, Kristine E. ; Redd, Andrew D. ; Quinn, Thomas C. ; Collinson-Streng, Aleisha N. ; Cornish, Toby ; Kong, Xiangrong ; Sharma, Rajni ; Tobian, Aaron A R ; Tsai, Benjamin ; Sherman, Mark E. ; Kigozi, Godfrey ; Serwadda, David ; Wawer, Maria J. ; Gray, Ronald H. / Effects of HIV-1 and herpes simplex virus type 2 infection on lymphocyte and dendritic cell density in adult foreskins from Rakai, Uganda. In: Journal of Infectious Diseases. 2011 ; Vol. 203, No. 5. pp. 602-609.
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abstract = "Background. Male circumcision reduces human immunodeficiency virus (HIV) and herpes simplex virus type 2 (HSV-2) acquisition, and HSV-2 infection is associated with an increased risk of HIV acquisition. To assess the cellular basis for these associations, we estimated immunologic cellular densities in foreskin tissue. Methods. Immunostained CD1a+ dendritic cell and CD4+ and CD8+ T cell densities were quantified in foreskin samples obtained from medical circumcision in Rakai, Uganda (35 HIV-infected, HSV-2-infected men; 5 HIV-infected, HSV-2-uninfected men; 22 HIV-uninfected, HSV-2-infected men; and 29 HIV-uninfected, HSV-2-uninfected men. Results. CD1A+ dendritic cell densities did not vary by HIV or HSV-2 status. Compared with densities in HIV-uninfected, HSV-2-uninfected men (mean, 26.8 cells/mm2), CD4+ T cell densities were similar in the HIV-infected, HSV-2-infected group (mean, 28.7 cells/mm2), were significantly decreased in the HIV-infected, HSV-2-uninfected group (mean, 11.2; P < .05), and were increased in the HIV-uninfected, HSV-2-infected group (mean, 68.7; P < .05). Dermal CD8+ T cell densities were higher in the HIV and HSV-2-coinfected group (mean, 102.9) than in the HIV-uninfected, HSV-2-uninfected group (mean, 10.0; P < .001), the HIV-infected, HSV-2-uninfected group (mean, 27.3; P < .001), and the HIV-uninfected, HSV-2-infected group (mean, 25.3; P < .005). Discussion. The increased CD4+ cellular density in the HIV-uninfected, HSV-2-infected men may help to explain why HSV-2-infected men are at increased risk of HIV acquisition. The absence of this increase in men coinfected with both HIV and HSV-2 is likely in part the result of the progressive loss of CD4+ cells in HIV infection. Conversely, HIV and HSV-2 coinfection appears to synergistically increase CD8+ T cell densities.",
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AU - Redd, Andrew D.

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AU - Collinson-Streng, Aleisha N.

AU - Cornish, Toby

AU - Kong, Xiangrong

AU - Sharma, Rajni

AU - Tobian, Aaron A R

AU - Tsai, Benjamin

AU - Sherman, Mark E.

AU - Kigozi, Godfrey

AU - Serwadda, David

AU - Wawer, Maria J.

AU - Gray, Ronald H.

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N2 - Background. Male circumcision reduces human immunodeficiency virus (HIV) and herpes simplex virus type 2 (HSV-2) acquisition, and HSV-2 infection is associated with an increased risk of HIV acquisition. To assess the cellular basis for these associations, we estimated immunologic cellular densities in foreskin tissue. Methods. Immunostained CD1a+ dendritic cell and CD4+ and CD8+ T cell densities were quantified in foreskin samples obtained from medical circumcision in Rakai, Uganda (35 HIV-infected, HSV-2-infected men; 5 HIV-infected, HSV-2-uninfected men; 22 HIV-uninfected, HSV-2-infected men; and 29 HIV-uninfected, HSV-2-uninfected men. Results. CD1A+ dendritic cell densities did not vary by HIV or HSV-2 status. Compared with densities in HIV-uninfected, HSV-2-uninfected men (mean, 26.8 cells/mm2), CD4+ T cell densities were similar in the HIV-infected, HSV-2-infected group (mean, 28.7 cells/mm2), were significantly decreased in the HIV-infected, HSV-2-uninfected group (mean, 11.2; P < .05), and were increased in the HIV-uninfected, HSV-2-infected group (mean, 68.7; P < .05). Dermal CD8+ T cell densities were higher in the HIV and HSV-2-coinfected group (mean, 102.9) than in the HIV-uninfected, HSV-2-uninfected group (mean, 10.0; P < .001), the HIV-infected, HSV-2-uninfected group (mean, 27.3; P < .001), and the HIV-uninfected, HSV-2-infected group (mean, 25.3; P < .005). Discussion. The increased CD4+ cellular density in the HIV-uninfected, HSV-2-infected men may help to explain why HSV-2-infected men are at increased risk of HIV acquisition. The absence of this increase in men coinfected with both HIV and HSV-2 is likely in part the result of the progressive loss of CD4+ cells in HIV infection. Conversely, HIV and HSV-2 coinfection appears to synergistically increase CD8+ T cell densities.

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