Effects of ginsenosides, active components of ginseng, on nicotinic acetylcholine receptors expressed in Xenopus oocytes

Seok Choi, Se Yeon Jung, Jun Ho Lee, Francisco Sala, Manuel Criado, Jose Mulet, Luis Miguel Valor, Salvador Sala, Andrew G Engel, Seung Yeol Nah

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

We investigated the effects of ginsenosides, the active ingredient of ginseng, on neuronal or muscle-type nicotinic acetylcholine receptor channel activity expressed in Xenopus oocytes after injection of cRNA encoding bovine neuronal α3β4, α7 or human muscle αβδε subunits. Treatment with acetylcholine elicited an inward peak current (IACh) in oocytes expressing nicotinic acetylcholine receptor subtypes. Cotreatment with ginsenoside Rg2 and acetylcholine inhibited IACh in oocytes expressing with α3β4 or αβδε but not in oocytes expressing α7 nicotinic acetylcholine receptors. The inhibition of IACh by ginsenoside Rg2 was reversible and dose-dependent. The half-inhibitory concentrations (IC50) of ginsenoside Rg2 were 60.2±14.1 and 15.7±3.5 μM in oocytes expressing α3β4 and αβδε nicotinic acetylcholine receptors, respectively. The inhibition of IACh by ginsenoside Rg2 was voltage-independent and noncompetitive. Other ginsenosides besides ginsenoside Rg2 also inhibited IACh in oocytes expressing α3β4 or αβδε nicotinic acetylcholine receptors. The order of potency for the inhibition of IACh was ginsenoside Rg2>Rf>Re>Rg1>Rc>Rb2>Rb 1 in oocytes expressing α3β4 nicotinic acetylcholine receptors and was ginsenoside Rg2>Rf>Rg1>Re>Rb1>Rc>Rb 2 in oocytes expressing αβδε nicotinic acetylcholine receptors. These results indicate that ginsenosides might regulate nicotinic acetylcholine receptors in a differential manner and this regulation might be one of the pharmacological actions of Panax ginseng.

Original languageEnglish (US)
Pages (from-to)37-45
Number of pages9
JournalEuropean Journal of Pharmacology
Volume442
Issue number1-2
DOIs
StatePublished - May 3 2002

Fingerprint

ginsenoside Rg2
Ginsenosides
Panax
Nicotinic Receptors
Xenopus
Oocytes
Acetylcholine
Muscles
Complementary RNA
Inhibitory Concentration 50

Keywords

  • Ginseng
  • Ginsenoside
  • Nicotinic acetylcholine receptor channel
  • Xenopus oocyte

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Effects of ginsenosides, active components of ginseng, on nicotinic acetylcholine receptors expressed in Xenopus oocytes. / Choi, Seok; Jung, Se Yeon; Lee, Jun Ho; Sala, Francisco; Criado, Manuel; Mulet, Jose; Valor, Luis Miguel; Sala, Salvador; Engel, Andrew G; Nah, Seung Yeol.

In: European Journal of Pharmacology, Vol. 442, No. 1-2, 03.05.2002, p. 37-45.

Research output: Contribution to journalArticle

Choi, Seok ; Jung, Se Yeon ; Lee, Jun Ho ; Sala, Francisco ; Criado, Manuel ; Mulet, Jose ; Valor, Luis Miguel ; Sala, Salvador ; Engel, Andrew G ; Nah, Seung Yeol. / Effects of ginsenosides, active components of ginseng, on nicotinic acetylcholine receptors expressed in Xenopus oocytes. In: European Journal of Pharmacology. 2002 ; Vol. 442, No. 1-2. pp. 37-45.
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AU - Criado, Manuel

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AU - Sala, Salvador

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