Effects of environmental anoxia on concentrations of free amino acids and kinetic properties of glutamate dehydrogenase in three body parts of Phascolosoma arcuatum (Sipuncula)

Y. K. Ip, S. F. Chew, Kah-Whye Peng, R. W L Lim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The body wall (BW), introvert cum retractor muscles (ICRM) and digestive tract (DT) of the sipunculid worm Phascolosoma arcuatum had different biochemical adaptations to anoxia with reference to amino acid metabolism. Although aspartate degradation occurred in all three body parts of the anoxic worm, alanine and glutamate accumulated only in the ICRM and DT, and not the BW. Hence, in the BW, the amino group of aspartate was not transferred to alanine during anoxia, but was possibly released as ammonia through glutamate. The kinetic properties of glutamate dehydrogenase (GDH) from the BW and ICRM were altered during anoxia to enhance the glutamate deaminating activity. Although alanine and glutamate were accumulated in the ICRM of the anoxic P. arcuatum, the decrease in aspartate content was unable to account for the increase. Results indicate that it was in the DT of the anoxic worm that glutamate was likely to act as an intermediate to shuttle the amino group between aspartate and alanine. The kinetic properties of the GDH from this body part of P. arcuatum were unaltered by anoxia.

Original languageEnglish (US)
Pages (from-to)125-132
Number of pages8
JournalJournal of Experimental Marine Biology and Ecology
Volume165
Issue number1
DOIs
StatePublished - Dec 21 1992
Externally publishedYes

Fingerprint

glutamate dehydrogenase
anoxia
glutamates
free amino acids
hypoxia
aspartic acid
muscle
integument
amino acid
alanine
kinetics
digestive tract
muscles
amino acid metabolism
ammonia
metabolism
degradation
effect

Keywords

  • Alanine
  • Anoxia
  • Aspartate
  • Glutamate dehydrogenase
  • Phascolosoma arcuatum
  • Sipunculid

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology, Evolution, Behavior and Systematics
  • Ecology

Cite this

@article{5ec9d118f5654364b8c2ff2c662b7a3d,
title = "Effects of environmental anoxia on concentrations of free amino acids and kinetic properties of glutamate dehydrogenase in three body parts of Phascolosoma arcuatum (Sipuncula)",
abstract = "The body wall (BW), introvert cum retractor muscles (ICRM) and digestive tract (DT) of the sipunculid worm Phascolosoma arcuatum had different biochemical adaptations to anoxia with reference to amino acid metabolism. Although aspartate degradation occurred in all three body parts of the anoxic worm, alanine and glutamate accumulated only in the ICRM and DT, and not the BW. Hence, in the BW, the amino group of aspartate was not transferred to alanine during anoxia, but was possibly released as ammonia through glutamate. The kinetic properties of glutamate dehydrogenase (GDH) from the BW and ICRM were altered during anoxia to enhance the glutamate deaminating activity. Although alanine and glutamate were accumulated in the ICRM of the anoxic P. arcuatum, the decrease in aspartate content was unable to account for the increase. Results indicate that it was in the DT of the anoxic worm that glutamate was likely to act as an intermediate to shuttle the amino group between aspartate and alanine. The kinetic properties of the GDH from this body part of P. arcuatum were unaltered by anoxia.",
keywords = "Alanine, Anoxia, Aspartate, Glutamate dehydrogenase, Phascolosoma arcuatum, Sipunculid",
author = "Ip, {Y. K.} and Chew, {S. F.} and Kah-Whye Peng and Lim, {R. W L}",
year = "1992",
month = "12",
day = "21",
doi = "10.1016/0022-0981(92)90293-J",
language = "English (US)",
volume = "165",
pages = "125--132",
journal = "Journal of Experimental Marine Biology and Ecology",
issn = "0022-0981",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Effects of environmental anoxia on concentrations of free amino acids and kinetic properties of glutamate dehydrogenase in three body parts of Phascolosoma arcuatum (Sipuncula)

AU - Ip, Y. K.

AU - Chew, S. F.

AU - Peng, Kah-Whye

AU - Lim, R. W L

PY - 1992/12/21

Y1 - 1992/12/21

N2 - The body wall (BW), introvert cum retractor muscles (ICRM) and digestive tract (DT) of the sipunculid worm Phascolosoma arcuatum had different biochemical adaptations to anoxia with reference to amino acid metabolism. Although aspartate degradation occurred in all three body parts of the anoxic worm, alanine and glutamate accumulated only in the ICRM and DT, and not the BW. Hence, in the BW, the amino group of aspartate was not transferred to alanine during anoxia, but was possibly released as ammonia through glutamate. The kinetic properties of glutamate dehydrogenase (GDH) from the BW and ICRM were altered during anoxia to enhance the glutamate deaminating activity. Although alanine and glutamate were accumulated in the ICRM of the anoxic P. arcuatum, the decrease in aspartate content was unable to account for the increase. Results indicate that it was in the DT of the anoxic worm that glutamate was likely to act as an intermediate to shuttle the amino group between aspartate and alanine. The kinetic properties of the GDH from this body part of P. arcuatum were unaltered by anoxia.

AB - The body wall (BW), introvert cum retractor muscles (ICRM) and digestive tract (DT) of the sipunculid worm Phascolosoma arcuatum had different biochemical adaptations to anoxia with reference to amino acid metabolism. Although aspartate degradation occurred in all three body parts of the anoxic worm, alanine and glutamate accumulated only in the ICRM and DT, and not the BW. Hence, in the BW, the amino group of aspartate was not transferred to alanine during anoxia, but was possibly released as ammonia through glutamate. The kinetic properties of glutamate dehydrogenase (GDH) from the BW and ICRM were altered during anoxia to enhance the glutamate deaminating activity. Although alanine and glutamate were accumulated in the ICRM of the anoxic P. arcuatum, the decrease in aspartate content was unable to account for the increase. Results indicate that it was in the DT of the anoxic worm that glutamate was likely to act as an intermediate to shuttle the amino group between aspartate and alanine. The kinetic properties of the GDH from this body part of P. arcuatum were unaltered by anoxia.

KW - Alanine

KW - Anoxia

KW - Aspartate

KW - Glutamate dehydrogenase

KW - Phascolosoma arcuatum

KW - Sipunculid

UR - http://www.scopus.com/inward/record.url?scp=0010278188&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0010278188&partnerID=8YFLogxK

U2 - 10.1016/0022-0981(92)90293-J

DO - 10.1016/0022-0981(92)90293-J

M3 - Article

AN - SCOPUS:0010278188

VL - 165

SP - 125

EP - 132

JO - Journal of Experimental Marine Biology and Ecology

JF - Journal of Experimental Marine Biology and Ecology

SN - 0022-0981

IS - 1

ER -