TY - JOUR
T1 - Effects of antithrombogenic agents on microvenous anastomoses in a rat model
AU - Harsha, Wayne J.
AU - Kau, Ryan L.
AU - Kim, Namou
AU - Hayden, Richard E.
PY - 2011/2
Y1 - 2011/2
N2 - Objective: To compare venous thrombosis rates among animals treated with aspirin, clopidogrel bisulfate, and ketorolac tromethamine using an anastomotic "tuck" model. Design: Single-blind randomized animal study. Setting: An animal laboratory at a tertiary care academic referral center. Subjects: Forty-two retired Lewis breeder rats divided into 3 equal groups. Interventions: Before surgical intervention, 1 group received aspirin (10 mg/kg) through gavage; 1 group, clopidogrel bisulfate (5 mg/kg) through gavage; and the final group, ketorolac tromethamine (3 mg/kg) through intramuscular injection. Each rat was then anesthetized, and the femoral veins were prepared bilaterally. A 180° venotomy was made, and the vessels were anastomosed with the tuck model set-up for anastomotic failure. The vessels were checked for patency every 15 minutes for 2 hours after clamp removal. Main Outcome Measures: The rate of venous thrombosis and the time to thrombosis. Results: In both the aspirin and clopidogrel groups, 2 of 28 vessels (7%) were thrombosed. Thrombosis occurred in 3 of 28 vessels (11%) in the ketorolac group (P=.86). All thromboses in the aspirin and clopidogrel groups took place at 7.5 minutes after clamp removal. In the ketorolac group, the mean time to thrombosis was 7.5 minutes (range, 0-22.5 minutes). There was no difference in time to thrombosis among the 3 groups (P=.86). Conclusion: Using a microvenous tuck model set-up for anastomotic failure, we found no difference in the rate of thrombosis or the time to thrombosis in rats pretreated with aspirin, clopidogrel, or ketorolac.
AB - Objective: To compare venous thrombosis rates among animals treated with aspirin, clopidogrel bisulfate, and ketorolac tromethamine using an anastomotic "tuck" model. Design: Single-blind randomized animal study. Setting: An animal laboratory at a tertiary care academic referral center. Subjects: Forty-two retired Lewis breeder rats divided into 3 equal groups. Interventions: Before surgical intervention, 1 group received aspirin (10 mg/kg) through gavage; 1 group, clopidogrel bisulfate (5 mg/kg) through gavage; and the final group, ketorolac tromethamine (3 mg/kg) through intramuscular injection. Each rat was then anesthetized, and the femoral veins were prepared bilaterally. A 180° venotomy was made, and the vessels were anastomosed with the tuck model set-up for anastomotic failure. The vessels were checked for patency every 15 minutes for 2 hours after clamp removal. Main Outcome Measures: The rate of venous thrombosis and the time to thrombosis. Results: In both the aspirin and clopidogrel groups, 2 of 28 vessels (7%) were thrombosed. Thrombosis occurred in 3 of 28 vessels (11%) in the ketorolac group (P=.86). All thromboses in the aspirin and clopidogrel groups took place at 7.5 minutes after clamp removal. In the ketorolac group, the mean time to thrombosis was 7.5 minutes (range, 0-22.5 minutes). There was no difference in time to thrombosis among the 3 groups (P=.86). Conclusion: Using a microvenous tuck model set-up for anastomotic failure, we found no difference in the rate of thrombosis or the time to thrombosis in rats pretreated with aspirin, clopidogrel, or ketorolac.
UR - http://www.scopus.com/inward/record.url?scp=79952012682&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79952012682&partnerID=8YFLogxK
U2 - 10.1001/archoto.2010.256
DO - 10.1001/archoto.2010.256
M3 - Article
C2 - 21339404
AN - SCOPUS:79952012682
SN - 0886-4470
VL - 137
SP - 170
EP - 174
JO - Archives of Otolaryngology - Head and Neck Surgery
JF - Archives of Otolaryngology - Head and Neck Surgery
IS - 2
ER -