Effects of 1,25-dihydroxyvitamin D3 on growth of mouse neuroblastoma cells

Timothy D. Veenstra, James M. Londowski, Anthony John Windebank, William Stephen Brimijoin, Rajiv Kumar

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Epitopes of the 1,25-dihydroxyvitamin D (1,25(OH)2D3) receptor have been shown in developing dorsal root ganglia in fetal mice, as well as in cells maintained in culture [Johnson, J.A., Grande, J.P., Windebank, A.J. and Kumar, R., 1,25-Dihydroxyvitamin D3 receptors in developing dorsal root ganglia of fetal rats, Dev. Brain Res., 92 (1996) 120-124]. To investigate a possible role for 1,25(OH)2D3 in neural cell growth and development, a murine neuroblastoma cell line that expresses 1,25(OH)2D3 receptors, was treated with 1,25(OH)2D3. Treatment with 1,25(OH)2D3 resulted in a decrease in cell proliferation, a change in cell morphology, and the expression of protein markers of mature neuronal cells. The decrease in cell proliferation was accompanied by an increase in the expression of nerve growth factor (NGF). Anti-NGF monoclonal antibody added to the growth medium blocked the decrease in cell proliferation caused by 1,25(OH)2D3 treatment. Our results show that the sterol hormone 1,25(OH)2D3, causes a decrease in the proliferation of mouse neuroblastoma cells through alterations in the expression of NGF.

Original languageEnglish (US)
Pages (from-to)53-60
Number of pages8
JournalDevelopmental Brain Research
Volume99
Issue number1
DOIs
StatePublished - Mar 17 1997

Fingerprint

Calcitriol
Neuroblastoma
Nerve Growth Factor
Growth
Cell Proliferation
Spinal Ganglia
Calcitriol Receptors
Sterols
Growth and Development
Epitopes
Monoclonal Antibodies
Hormones
Cell Line
Brain
Proteins

Keywords

  • 1,25-dihydroxyvitamin D
  • decrease in proliferation
  • neuroblastoma
  • NGF

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Effects of 1,25-dihydroxyvitamin D3 on growth of mouse neuroblastoma cells. / Veenstra, Timothy D.; Londowski, James M.; Windebank, Anthony John; Brimijoin, William Stephen; Kumar, Rajiv.

In: Developmental Brain Research, Vol. 99, No. 1, 17.03.1997, p. 53-60.

Research output: Contribution to journalArticle

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