TY - JOUR
T1 - Effects of 1,25-dihydroxyvitamin D3 on growth of mouse neuroblastoma cells
AU - Veenstra, Timothy D.
AU - Londowski, James M.
AU - Windebank, Anthony J.
AU - Brimijoin, Stephen
AU - Kumar, Rajiv
N1 - Funding Information:
We would like to thank Steven C. Zeismer for expert assistance in performing the immunohistochemical techniques. Supported by NIH Grants: DK25409 (R.K.), DK42971 (R.K.)
PY - 1997/3/17
Y1 - 1997/3/17
N2 - Epitopes of the 1,25-dihydroxyvitamin D (1,25(OH)2D3) receptor have been shown in developing dorsal root ganglia in fetal mice, as well as in cells maintained in culture [Johnson, J.A., Grande, J.P., Windebank, A.J. and Kumar, R., 1,25-Dihydroxyvitamin D3 receptors in developing dorsal root ganglia of fetal rats, Dev. Brain Res., 92 (1996) 120-124]. To investigate a possible role for 1,25(OH)2D3 in neural cell growth and development, a murine neuroblastoma cell line that expresses 1,25(OH)2D3 receptors, was treated with 1,25(OH)2D3. Treatment with 1,25(OH)2D3 resulted in a decrease in cell proliferation, a change in cell morphology, and the expression of protein markers of mature neuronal cells. The decrease in cell proliferation was accompanied by an increase in the expression of nerve growth factor (NGF). Anti-NGF monoclonal antibody added to the growth medium blocked the decrease in cell proliferation caused by 1,25(OH)2D3 treatment. Our results show that the sterol hormone 1,25(OH)2D3, causes a decrease in the proliferation of mouse neuroblastoma cells through alterations in the expression of NGF.
AB - Epitopes of the 1,25-dihydroxyvitamin D (1,25(OH)2D3) receptor have been shown in developing dorsal root ganglia in fetal mice, as well as in cells maintained in culture [Johnson, J.A., Grande, J.P., Windebank, A.J. and Kumar, R., 1,25-Dihydroxyvitamin D3 receptors in developing dorsal root ganglia of fetal rats, Dev. Brain Res., 92 (1996) 120-124]. To investigate a possible role for 1,25(OH)2D3 in neural cell growth and development, a murine neuroblastoma cell line that expresses 1,25(OH)2D3 receptors, was treated with 1,25(OH)2D3. Treatment with 1,25(OH)2D3 resulted in a decrease in cell proliferation, a change in cell morphology, and the expression of protein markers of mature neuronal cells. The decrease in cell proliferation was accompanied by an increase in the expression of nerve growth factor (NGF). Anti-NGF monoclonal antibody added to the growth medium blocked the decrease in cell proliferation caused by 1,25(OH)2D3 treatment. Our results show that the sterol hormone 1,25(OH)2D3, causes a decrease in the proliferation of mouse neuroblastoma cells through alterations in the expression of NGF.
KW - 1,25-dihydroxyvitamin D
KW - NGF
KW - decrease in proliferation
KW - neuroblastoma
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U2 - 10.1016/S0165-3806(96)00196-4
DO - 10.1016/S0165-3806(96)00196-4
M3 - Article
C2 - 9088565
AN - SCOPUS:0031054504
SN - 0165-3806
VL - 99
SP - 53
EP - 60
JO - Developmental Brain Research
JF - Developmental Brain Research
IS - 1
ER -