Effect of the effluent released from the canine internal mammary artery after intraluminal and extraluminal perfusion of acetylcholine and adenosine diphosphate

Nilce Mitiko Matsuda, Paul J. Pearson, Hartzell V Schaff, Carlos E. Piccinato, Alfredo J. Rodrigues, Paulo Roberto Barbosa Evora

Research output: Contribution to journalArticle

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Abstract

Segments of the canine internal mammary artery (35 mm in length) were suspended in vitro in an organ chamber containing physiological salt solution (95% O2/5% CO2, pH = 7.4, 37°C). Segments were individually cannulated and perfused at 5 ml/minute using a roller pump. Vasorelaxant activity of the effluent from the perfused internal mammary arteries was bioassayed by measuring the decrease in tension induced by the effluent of the coronary artery endothelium-free ring which had been contracted with prostaglandin F2 (2 × 10-6M). Intraluminal perfusion of adenosine diphosphate (10 -5M) induced significant increase in relaxant activity in the effluent from the perfused blood vessel. However, when adenosine diphosphate (10-5M) was added extraluminally to the internal mammary artery, no change in relaxant activity in the effluent was noted. In contrast, acetylcholine produced significant increase in the relaxant activity on the effluent of the perfused internal mammary artery with both intraluminal and extraluminal perfusion. The intraluminal and extraluminal release of endothelium-derived relaxing factor (EDRF) by acetylcholine (10-5M) can be inhibited by site-specific administration of atropine (10-5M). These experiments indicate that certain agonists can induce the release of EDRF only by binding to intravascular receptors while other agonists can induce endothelium-dependent vasodilatation by acting on neural side receptors.

Original languageEnglish (US)
Article number45
JournalJournal of Biomedical Science
Volume16
Issue number1
DOIs
StatePublished - 2009

Fingerprint

Mammary Arteries
Adenosine Diphosphate
Acetylcholine
Canidae
Effluents
Perfusion
Endothelium-Dependent Relaxing Factors
Endothelium
Dinoprost
Sensory Receptor Cells
Vasodilator Agents
Atropine
Vasodilation
Blood Vessels
Blood vessels
Coronary Vessels
Salts
Pumps
Experiments

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Cell Biology
  • Biochemistry, medical
  • Endocrinology, Diabetes and Metabolism
  • Pharmacology (medical)

Cite this

Effect of the effluent released from the canine internal mammary artery after intraluminal and extraluminal perfusion of acetylcholine and adenosine diphosphate. / Matsuda, Nilce Mitiko; Pearson, Paul J.; Schaff, Hartzell V; Piccinato, Carlos E.; Rodrigues, Alfredo J.; Evora, Paulo Roberto Barbosa.

In: Journal of Biomedical Science, Vol. 16, No. 1, 45, 2009.

Research output: Contribution to journalArticle

Matsuda, Nilce Mitiko ; Pearson, Paul J. ; Schaff, Hartzell V ; Piccinato, Carlos E. ; Rodrigues, Alfredo J. ; Evora, Paulo Roberto Barbosa. / Effect of the effluent released from the canine internal mammary artery after intraluminal and extraluminal perfusion of acetylcholine and adenosine diphosphate. In: Journal of Biomedical Science. 2009 ; Vol. 16, No. 1.
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