TY - JOUR
T1 - Effect of the effluent released from the canine internal mammary artery after intraluminal and extraluminal perfusion of acetylcholine and adenosine diphosphate
AU - Matsuda, Nilce Mitiko
AU - Pearson, Paul J.
AU - Schaff, Hartzell V.
AU - Piccinato, Carlos E.
AU - Rodrigues, Alfredo J.
AU - Evora, Paulo Roberto Barbosa
N1 - Funding Information:
This manuscript was supported by grants from Fundação de Amparo a Pesquisas do Estado de São Paulo (FAPESP 2006/50084-2) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq 474531/ 2008-2) to NM Matsuda.
PY - 2009
Y1 - 2009
N2 - Segments of the canine internal mammary artery (35 mm in length) were suspended in vitro in an organ chamber containing physiological salt solution (95% O2/5% CO2, pH = 7.4, 37°C). Segments were individually cannulated and perfused at 5 ml/minute using a roller pump. Vasorelaxant activity of the effluent from the perfused internal mammary arteries was bioassayed by measuring the decrease in tension induced by the effluent of the coronary artery endothelium-free ring which had been contracted with prostaglandin F2 (2 × 10-6M). Intraluminal perfusion of adenosine diphosphate (10 -5M) induced significant increase in relaxant activity in the effluent from the perfused blood vessel. However, when adenosine diphosphate (10-5M) was added extraluminally to the internal mammary artery, no change in relaxant activity in the effluent was noted. In contrast, acetylcholine produced significant increase in the relaxant activity on the effluent of the perfused internal mammary artery with both intraluminal and extraluminal perfusion. The intraluminal and extraluminal release of endothelium-derived relaxing factor (EDRF) by acetylcholine (10-5M) can be inhibited by site-specific administration of atropine (10-5M). These experiments indicate that certain agonists can induce the release of EDRF only by binding to intravascular receptors while other agonists can induce endothelium-dependent vasodilatation by acting on neural side receptors.
AB - Segments of the canine internal mammary artery (35 mm in length) were suspended in vitro in an organ chamber containing physiological salt solution (95% O2/5% CO2, pH = 7.4, 37°C). Segments were individually cannulated and perfused at 5 ml/minute using a roller pump. Vasorelaxant activity of the effluent from the perfused internal mammary arteries was bioassayed by measuring the decrease in tension induced by the effluent of the coronary artery endothelium-free ring which had been contracted with prostaglandin F2 (2 × 10-6M). Intraluminal perfusion of adenosine diphosphate (10 -5M) induced significant increase in relaxant activity in the effluent from the perfused blood vessel. However, when adenosine diphosphate (10-5M) was added extraluminally to the internal mammary artery, no change in relaxant activity in the effluent was noted. In contrast, acetylcholine produced significant increase in the relaxant activity on the effluent of the perfused internal mammary artery with both intraluminal and extraluminal perfusion. The intraluminal and extraluminal release of endothelium-derived relaxing factor (EDRF) by acetylcholine (10-5M) can be inhibited by site-specific administration of atropine (10-5M). These experiments indicate that certain agonists can induce the release of EDRF only by binding to intravascular receptors while other agonists can induce endothelium-dependent vasodilatation by acting on neural side receptors.
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U2 - 10.1186/1423-0127-16-45
DO - 10.1186/1423-0127-16-45
M3 - Article
C2 - 19416519
AN - SCOPUS:66649102131
SN - 1021-7770
VL - 16
JO - Journal of Biomedical Science
JF - Journal of Biomedical Science
IS - 1
M1 - 45
ER -