Effect of the Artificial Sweetener, Acesulfame Potassium, a Sweet Taste Receptor Agonist, on Glucose Uptake in Small Intestinal Cell Lines

Ye Zheng, Michael G. Sarr

Research output: Contribution to journalArticle

19 Scopus citations


Introduction: Sweet taste receptors may enhance glucose absorption. Aim: This study aimed to explore the cell biology of sweet taste receptors on glucose uptake. Hypothesis: Artificial sweeteners increase glucose uptake via activating sweet taste receptors in the enterocyte to translocate GLUT2 to the apical membrane through the PLC βII pathway. Methods: Caco-2, RIE-1, and IEC-6 cells, starved from glucose for 1 h were pre-incubated with 10 mM acesulfame potassium (AceK). Glucose uptake was measured by incubating cells for 1 to 10 min with 0. 5-50 mM glucose with or without U-73122, chelerythrine, and cytochalasin B. Results: In Caco-2 and RIE-1 cells, 10 mM AceK increased glucose uptake by 20-30 % at glucose >25 mM, but not in lesser glucose concentrations (<10 mM), nor at 1 min or 10 min incubations. U-73122 (PLC βII inhibitor) inhibited uptake at glucose >25 mM and for 5 min incubation; chelerythrine and cytochalasin B had similar effects. No effect occurred in IEC-6 cells. Summary: Activation of sweet taste receptors had no effect on glucose uptake in low (<25 mM) glucose concentrations but increased uptake at greater concentrations (>25 mM). Conclusions: Role of artificial sweeteners on glucose uptake appears to act in part by effects on the enterocyte itself.

Original languageEnglish (US)
Pages (from-to)153-158
Number of pages6
JournalJournal of Gastrointestinal Surgery
Issue number1
StatePublished - Jan 10 2013



  • Acesulfame potassium
  • GLUT2
  • PLC βII
  • Sweet taste receptor

ASJC Scopus subject areas

  • Surgery
  • Gastroenterology

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