Effect of some factors on determination of viable microbial cells by peroxyoxalate chemiluminescence method

Sang Kon Oh, Seung Hee Cha

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The effects of physical and chemical factors on the production of H2O2 from Escherichia coli cells were studied. When 20 mmol 1‐1 Tris‐HCl buffer was used for this purpose the electron transport system (ETS) showed the highest activity at pH 7.6‐8.2. KCN promoted the production of H2O2 from E. coli cells, and the optimum concentration was changed in different reaction times and pH values. Glucose, 5 mg ml‐1, increased the ETS activity about twofold. The other substrates and surfactants did not increase the chemiluminescence intensity. NaNO2 and Na2SO4 in inorganic salts significantly reduced the ETS activity above 70%. In addition, the optimum temperature for the production of H2O2 was 30°C in this study. When glucose (5 mg ml‐1) and KCN (0.2 mmol 1‐1) were added to the reaction buffer containing 0.5 mmol 1‐1 menadione, the detectable minimum cell densities (averages of triplicate assay) of E. coli, Enterobacter cloacae and Serratia marcescens were 5 times 103 cells ml‐1, 104 cells ml‐1 and 104 cells ml‐1 respectively.

Original languageEnglish (US)
Pages (from-to)275-281
Number of pages7
JournalJournal of Applied Bacteriology
Volume76
Issue number3
DOIs
StatePublished - Mar 1994

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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