Abstract
Introduction: These studies were designed to determine whether PJ34, a novel Poly-ADP Ribose Polymerase Inhibitor, modulates expression of markers of stress and inflammation in the spinal cord following ischemia/ reperfusion(TAR). Methods: 129S1/SvImj mice were subjected to thoracic aortic occlusion and 48 hours of reperfusion (n = 38). Experimental Groups included: Untreated Control (UC, n = 21); PJ34 (PJ34, n = 11) and sham (S, n = 6). At 48 hours, mice were euthanized for mRNA analysis and assessment of spinal cord viability. Results: PJ34 improved spinal cord tissue viability following TAR (UC:53.1 ± 6.3, PJ34:73.5 ± 4.1% sham, p < 0.01). mRNA analysis revealed significant expression of stress response genes in UC and PJ34 treated mice. Conclusions: PJ34 enhanced mitochondrial activity and preserved neurologic function following TAR despite the expression of stress and pro-inflammatory markers within the spinal cord. The ongoing cord stress response in neurologically intact PJ34 treated mice may indicate the potential to develop delayed neurologic dysfunction.
Original language | English (US) |
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Pages (from-to) | 444-451 |
Number of pages | 8 |
Journal | Vascular and Endovascular Surgery |
Volume | 43 |
Issue number | 5 |
DOIs | |
State | Published - Oct 2009 |
Keywords
- Inflammation
- Ischemia
- Paralysis
- Poly ADP-ribose polymerase
- Reperfusion injury
ASJC Scopus subject areas
- Surgery
- Cardiology and Cardiovascular Medicine