Effect of opioid peptides on circular muscle of canine duodenum

A. J. Bauer, J. H. Szurszewski

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The effects of opioid peptides on inhibitory transmission in the circular muscle layer of canine duodenum were investigated in vitro using simultaneous mechanical and intracellular electrical recording techniques. Exogenously added [Met5]enkephalin, [Leu5]enkephalin and dynorphin (1-13) decreased the amplitude of non-adrenergic, non-cholinergic inhibitory junction potentials (IJPs) evoked by transmural nerve stimulation. A selective δ-receptor agonist, DPDPE ([D-Pen2, D-Pen5]enkephalin), and a selective μ-receptor agonist, PL017 (Try-Pro-NMePhe-D-Pro-NH2), decreased the amplitude of IJPs whereas a selective κ-receptor agonist, U-50,488H ([trans-3,4-dichloro-N-methyl-N-(2-91-pyrolidinyl)-cyclohexyl]-benzene acetamide methanesulphonate), in large doses (1 μM) produced only a small reduction. A selective δ-receptor antagonist, ICI-174,864, blocked the effect of DPDPE but not that of PL017 suggesting the presence of distinct δ- and μ-opioid receptors on inhibitory motor nerves. Exogenously added dynorphin (1-13) decreased the amplitude of IJPs. δ-Opioid receptors appeared to be involved because ICI-174,864, a selective δ-antagonist, blocked the inhibitory effect of exogenously added dynorphin (1-13). The inhibitory effect of the opioid peptides was still observed in preparations of circular muscle devoid of myenteric and submucosal plexuses, indicating that the site of action was on inhibitory motor nerve fibres located within the circular muscle layer and not on neuronal cell bodies in the enteric plexuses. It was concluded that in the canine small intestine, opioid peptides could modulate release of inhibitory transmitter(s) at or near nerve terminals of inhibitory motor nerves innervating circular muscle cells.

Original languageEnglish (US)
Pages (from-to)409-422
Number of pages14
JournalJournal of Physiology
Volume434
StatePublished - 1991

Fingerprint

Opioid Peptides
Duodenum
D-Penicillamine (2,5)-Enkephalin
Canidae
Enkephalins
Opioid Receptors
Muscles
(trans)-Isomer 3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide
Submucous Plexus
Myenteric Plexus
Benzene
Nerve Fibers
Evoked Potentials
Muscle Cells
Small Intestine
dynorphin (1-13)

ASJC Scopus subject areas

  • Physiology

Cite this

Bauer, A. J., & Szurszewski, J. H. (1991). Effect of opioid peptides on circular muscle of canine duodenum. Journal of Physiology, 434, 409-422.

Effect of opioid peptides on circular muscle of canine duodenum. / Bauer, A. J.; Szurszewski, J. H.

In: Journal of Physiology, Vol. 434, 1991, p. 409-422.

Research output: Contribution to journalArticle

Bauer, AJ & Szurszewski, JH 1991, 'Effect of opioid peptides on circular muscle of canine duodenum', Journal of Physiology, vol. 434, pp. 409-422.
Bauer, A. J. ; Szurszewski, J. H. / Effect of opioid peptides on circular muscle of canine duodenum. In: Journal of Physiology. 1991 ; Vol. 434. pp. 409-422.
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