Effect of nitric oxide on circular muscle of the canine small intestine

M. E. Stark, A. J. Bauer, J. H. Szurszewski

Research output: Contribution to journalArticle

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Abstract

1. Experiments were designed to determine in circular muscle of the canine jejunum whether exogenous nitric oxide (NO) mimics the non-adrenergic, non-cholinergic inhibitory junction potential (NANC IJP), and whether changes in the availability of endogenous NO affects IJP amplitude. 2. Mechanical and intracellular electrical activity were recorded simultaneously from circular muscle of the canine jejunum. Electrical field stimulation evoked NANC IJPs and inhibited spontaneous contractions. 3. Infusions of NO solutions evoked immediate dose-dependent and transient hyperpolarizations and transiently inhibited spontaneous contractions. NO-evoked hyperpolarizations were unaffected by atropine, propranolol, phentolamine and tetrodotoxin. 4. The maximum IJP amplitude and the maximum amplitude of NO-evoked hyperpolarization were similar. 5. N(G)-mono-methyl-L-arginine (L-NMMA), which inhibits synthesis of NO from L-arginine, reduced IJP amplitudes but did not reduce the response to exogenous NO. L-arginine, but not D-arginine, reversed the effect of L-NMMA on IJP amplitude. 6. Oxyhaemoglobin, which binds and inactivates NO, reduced IJP amplitude and abolished the response to exogenous NO. 7. Exogenous NO mimicked the effects of NANC inhibitory input. Reducing the availability of endogenous NO reduced NANC inhibitory input. 8. It was concluded that NO mediates NANC neural inhibition and may act as a NANC inhibitory neurotransmitter in the canine jejunum.

Original languageEnglish (US)
Pages (from-to)743-761
Number of pages19
JournalJournal of Physiology
Volume444
StatePublished - 1991

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Small Intestine
Canidae
Nitric Oxide
Muscles
Arginine
Jejunum
omega-N-Methylarginine
Neural Inhibition
Oxyhemoglobins
Phentolamine
Tetrodotoxin
Atropine
Propranolol
Electric Stimulation
Neurotransmitter Agents

ASJC Scopus subject areas

  • Physiology

Cite this

Stark, M. E., Bauer, A. J., & Szurszewski, J. H. (1991). Effect of nitric oxide on circular muscle of the canine small intestine. Journal of Physiology, 444, 743-761.

Effect of nitric oxide on circular muscle of the canine small intestine. / Stark, M. E.; Bauer, A. J.; Szurszewski, J. H.

In: Journal of Physiology, Vol. 444, 1991, p. 743-761.

Research output: Contribution to journalArticle

Stark, ME, Bauer, AJ & Szurszewski, JH 1991, 'Effect of nitric oxide on circular muscle of the canine small intestine', Journal of Physiology, vol. 444, pp. 743-761.
Stark, M. E. ; Bauer, A. J. ; Szurszewski, J. H. / Effect of nitric oxide on circular muscle of the canine small intestine. In: Journal of Physiology. 1991 ; Vol. 444. pp. 743-761.
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