Effect of multiple freeze-thaw cycles on detection of measles, mumps, and rubella virus antibodies

Norman A. Pinsky, Jeanne M. Huddleston, Robert M. Jacobson, Peter C. Wollan, Gregory A. Poland

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

We investigated the effect of multiple freeze-thaw cycles on mumps, measles, and rubella virus serum antibody levels with whole-virus immunoglobulin G enzyme-linked immunoassays. Fresh serum samples from nine healthy adult volunteers were divided into six sets of five aliquots each. Samples were taken through a total of 10 freeze-thaw cycles and stored at 4°C until assayed. Each assay measurement was done in replicates of five, and the mean value was reported. After completing 10 freeze-thaw cycles, we found no clinically or statistically significant effect on measured antibody levels and found no discernible detrimental effect on the ability to measure these antibodies by enzyme-linked immunoassays.

Original languageEnglish (US)
Pages (from-to)19-21
Number of pages3
JournalClinical and Diagnostic Laboratory Immunology
Volume10
Issue number1
DOIs
StatePublished - Jan 2003

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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