Effect of multiple freeze-thaw cycles on detection of measles, mumps, and rubella virus antibodies

Norman A. Pinsky, Jeanne M. Huddleston, Robert M. Jacobson, Peter C. Wollan, Gregory A. Poland

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We investigated the effect of multiple freeze-thaw cycles on mumps, measles, and rubella virus serum antibody levels with whole-virus immunoglobulin G enzyme-linked immunoassays. Fresh serum samples from nine healthy adult volunteers were divided into six sets of five aliquots each. Samples were taken through a total of 10 freeze-thaw cycles and stored at 4°C until assayed. Each assay measurement was done in replicates of five, and the mean value was reported. After completing 10 freeze-thaw cycles, we found no clinically or statistically significant effect on measured antibody levels and found no discernible detrimental effect on the ability to measure these antibodies by enzyme-linked immunoassays.

Original languageEnglish (US)
Pages (from-to)19-21
Number of pages3
JournalClinical and Diagnostic Laboratory Immunology
Volume10
Issue number1
DOIs
StatePublished - Jan 2003
Externally publishedYes

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Mumps virus
Rubella virus
Measles virus
Viruses
Immunoenzyme Techniques
Antibodies
Enzymes
Serum
Assays
Healthy Volunteers
Immunoglobulin G
rubella antibodies

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology and Allergy
  • Clinical Biochemistry
  • Immunology

Cite this

Effect of multiple freeze-thaw cycles on detection of measles, mumps, and rubella virus antibodies. / Pinsky, Norman A.; Huddleston, Jeanne M.; Jacobson, Robert M.; Wollan, Peter C.; Poland, Gregory A.

In: Clinical and Diagnostic Laboratory Immunology, Vol. 10, No. 1, 01.2003, p. 19-21.

Research output: Contribution to journalArticle

Pinsky, Norman A. ; Huddleston, Jeanne M. ; Jacobson, Robert M. ; Wollan, Peter C. ; Poland, Gregory A. / Effect of multiple freeze-thaw cycles on detection of measles, mumps, and rubella virus antibodies. In: Clinical and Diagnostic Laboratory Immunology. 2003 ; Vol. 10, No. 1. pp. 19-21.
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