Effect of motilin and erythromycin on calcium-activated potassium channels in rabbit colonic myocytes

G. Lu, M. G. Sarr, J. H. Szurszewski

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background and Aims: Motilin and erythromycin are prokinetic agents that act on the same receptor in gastrointestinal smooth muscle to cause contraction. Both agonists may also cause an increase in outward current. The aim of this study was to determine whether motilin and erythromycin activate calcium-activated potassium (K(Ca)) channels. Methods: Freshly dispersed longitudinal smooth muscle cells of the rabbit colon were used to measure whole-cell outward current and single-channel activity using patch clamp recording methods. Results: Erythromycin and motilin increased a calcium- dependent outward potassium current and increased the open probability of K(Ca) channels of cell-attached patches. Conclusions: Erythromycin and motilin activate K(Ca) channels via an intracellular second messenger system. This effect may modulate the increase in contractility caused by these agonists.

Original languageEnglish (US)
Pages (from-to)748-754
Number of pages7
JournalGastroenterology
Volume114
Issue number4 I
DOIs
StatePublished - 1998

Fingerprint

Motilin
Calcium-Activated Potassium Channels
Erythromycin
Muscle Cells
Rabbits
Second Messenger Systems
Potassium
Calcium
Smooth Muscle Myocytes
Smooth Muscle
Colon

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Effect of motilin and erythromycin on calcium-activated potassium channels in rabbit colonic myocytes. / Lu, G.; Sarr, M. G.; Szurszewski, J. H.

In: Gastroenterology, Vol. 114, No. 4 I, 1998, p. 748-754.

Research output: Contribution to journalArticle

Lu, G. ; Sarr, M. G. ; Szurszewski, J. H. / Effect of motilin and erythromycin on calcium-activated potassium channels in rabbit colonic myocytes. In: Gastroenterology. 1998 ; Vol. 114, No. 4 I. pp. 748-754.
@article{0883d8c225c34e4aa52082c03f783d35,
title = "Effect of motilin and erythromycin on calcium-activated potassium channels in rabbit colonic myocytes",
abstract = "Background and Aims: Motilin and erythromycin are prokinetic agents that act on the same receptor in gastrointestinal smooth muscle to cause contraction. Both agonists may also cause an increase in outward current. The aim of this study was to determine whether motilin and erythromycin activate calcium-activated potassium (K(Ca)) channels. Methods: Freshly dispersed longitudinal smooth muscle cells of the rabbit colon were used to measure whole-cell outward current and single-channel activity using patch clamp recording methods. Results: Erythromycin and motilin increased a calcium- dependent outward potassium current and increased the open probability of K(Ca) channels of cell-attached patches. Conclusions: Erythromycin and motilin activate K(Ca) channels via an intracellular second messenger system. This effect may modulate the increase in contractility caused by these agonists.",
author = "G. Lu and Sarr, {M. G.} and Szurszewski, {J. H.}",
year = "1998",
doi = "10.1016/S0016-5085(98)70588-8",
language = "English (US)",
volume = "114",
pages = "748--754",
journal = "Gastroenterology",
issn = "0016-5085",
publisher = "W.B. Saunders Ltd",
number = "4 I",

}

TY - JOUR

T1 - Effect of motilin and erythromycin on calcium-activated potassium channels in rabbit colonic myocytes

AU - Lu, G.

AU - Sarr, M. G.

AU - Szurszewski, J. H.

PY - 1998

Y1 - 1998

N2 - Background and Aims: Motilin and erythromycin are prokinetic agents that act on the same receptor in gastrointestinal smooth muscle to cause contraction. Both agonists may also cause an increase in outward current. The aim of this study was to determine whether motilin and erythromycin activate calcium-activated potassium (K(Ca)) channels. Methods: Freshly dispersed longitudinal smooth muscle cells of the rabbit colon were used to measure whole-cell outward current and single-channel activity using patch clamp recording methods. Results: Erythromycin and motilin increased a calcium- dependent outward potassium current and increased the open probability of K(Ca) channels of cell-attached patches. Conclusions: Erythromycin and motilin activate K(Ca) channels via an intracellular second messenger system. This effect may modulate the increase in contractility caused by these agonists.

AB - Background and Aims: Motilin and erythromycin are prokinetic agents that act on the same receptor in gastrointestinal smooth muscle to cause contraction. Both agonists may also cause an increase in outward current. The aim of this study was to determine whether motilin and erythromycin activate calcium-activated potassium (K(Ca)) channels. Methods: Freshly dispersed longitudinal smooth muscle cells of the rabbit colon were used to measure whole-cell outward current and single-channel activity using patch clamp recording methods. Results: Erythromycin and motilin increased a calcium- dependent outward potassium current and increased the open probability of K(Ca) channels of cell-attached patches. Conclusions: Erythromycin and motilin activate K(Ca) channels via an intracellular second messenger system. This effect may modulate the increase in contractility caused by these agonists.

UR - http://www.scopus.com/inward/record.url?scp=0031924158&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031924158&partnerID=8YFLogxK

U2 - 10.1016/S0016-5085(98)70588-8

DO - 10.1016/S0016-5085(98)70588-8

M3 - Article

C2 - 9516395

AN - SCOPUS:0031924158

VL - 114

SP - 748

EP - 754

JO - Gastroenterology

JF - Gastroenterology

SN - 0016-5085

IS - 4 I

ER -