Effect of intracerebral injection of monoclonal acetylcholinesterase antibodies on cholinergic nerve terminals in the rat central nervous system

Andrew J. Bean; Zhang Xu; Siew Y. Chai; Stephen Brimijoin; Tomas Hökfelt

doi:10.1016/0304-3940(91)90078-8

Effect of intracerebral injection of monoclonal acetylcholinesterase antibodies on cholinergic nerve terminals in the rat central nervous system

Andrew J. Bean, Zhang Xu, Siew Y. Chai, Stephen Brimijoin, Tomas Hökfelt

Pharmacology

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

In the rat, unilateral intrastriatal injection of monoclonal antibodies to acetylcholinesterase (AChE) produced ipsilateral disappearance of AChE-positive nerve terminals within striatum and adjacent cortex. No alterations in striatal staining patterns were observed for tyrosine hydroxylase, somatostatin, neuropeptide Y, substance P, or neurotensin. Ultrastructural studies demonstrated the presence of degenerating AChE-positive boutons ipsilaterally, while tyrosine hydroxylase positive terminals seemed unaffected. Apomorphine administration to rats which had received unilateral antibody injection resulted in ipsilateral rotational behavior. These data suggest that selective effects on cholinergic terminals with functional deficits can be produced within the central nervous system by intracerebral injection of AChE antibodies.

Original language	English (US)
Pages (from-to)	145-149
Number of pages	5
Journal	Neuroscience Letters
Volume	133
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(91)90078-8
State	Published - Nov 25 1991

Keywords

Acetylcholine
Dopamine
Rat
Rotational behavior
Striatum
Tyrosine hydroxylase

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(91)90078-8

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title = "Effect of intracerebral injection of monoclonal acetylcholinesterase antibodies on cholinergic nerve terminals in the rat central nervous system",

abstract = "In the rat, unilateral intrastriatal injection of monoclonal antibodies to acetylcholinesterase (AChE) produced ipsilateral disappearance of AChE-positive nerve terminals within striatum and adjacent cortex. No alterations in striatal staining patterns were observed for tyrosine hydroxylase, somatostatin, neuropeptide Y, substance P, or neurotensin. Ultrastructural studies demonstrated the presence of degenerating AChE-positive boutons ipsilaterally, while tyrosine hydroxylase positive terminals seemed unaffected. Apomorphine administration to rats which had received unilateral antibody injection resulted in ipsilateral rotational behavior. These data suggest that selective effects on cholinergic terminals with functional deficits can be produced within the central nervous system by intracerebral injection of AChE antibodies.",

keywords = "Acetylcholine, Dopamine, Rat, Rotational behavior, Striatum, Tyrosine hydroxylase",

author = "Bean, {Andrew J.} and Zhang Xu and Chai, {Siew Y.} and Stephen Brimijoin and Tomas H{\"o}kfelt",

note = "Funding Information: We wish to thank K. Aman, K. Friberg, W. Hiort and S. Nilsson for excellent technical assistance, Pamela Hammond for purifying the monoclonal antibodies, Professor L. Olson for the use of the rotometers, and Drs. R. Elde, L. Jennes, J. Massoulie, and L. Terenius for generous supplies of antisera. These studies were supported in part by The Pharmaceutical Manufacturers Association Foundation, the Scottish Rite Schizophrenia Research Foundation, the Swedish Medical Research Council (2887), the USPHS (MH-43230) and (NS-29646), and the C.J. Martin Fellowship from the National Health and Medical Research Council of Australia (S.Y.C.).",

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AU - Brimijoin, Stephen

AU - Hökfelt, Tomas

N1 - Funding Information: We wish to thank K. Aman, K. Friberg, W. Hiort and S. Nilsson for excellent technical assistance, Pamela Hammond for purifying the monoclonal antibodies, Professor L. Olson for the use of the rotometers, and Drs. R. Elde, L. Jennes, J. Massoulie, and L. Terenius for generous supplies of antisera. These studies were supported in part by The Pharmaceutical Manufacturers Association Foundation, the Scottish Rite Schizophrenia Research Foundation, the Swedish Medical Research Council (2887), the USPHS (MH-43230) and (NS-29646), and the C.J. Martin Fellowship from the National Health and Medical Research Council of Australia (S.Y.C.).

PY - 1991/11/25

Y1 - 1991/11/25

N2 - In the rat, unilateral intrastriatal injection of monoclonal antibodies to acetylcholinesterase (AChE) produced ipsilateral disappearance of AChE-positive nerve terminals within striatum and adjacent cortex. No alterations in striatal staining patterns were observed for tyrosine hydroxylase, somatostatin, neuropeptide Y, substance P, or neurotensin. Ultrastructural studies demonstrated the presence of degenerating AChE-positive boutons ipsilaterally, while tyrosine hydroxylase positive terminals seemed unaffected. Apomorphine administration to rats which had received unilateral antibody injection resulted in ipsilateral rotational behavior. These data suggest that selective effects on cholinergic terminals with functional deficits can be produced within the central nervous system by intracerebral injection of AChE antibodies.

AB - In the rat, unilateral intrastriatal injection of monoclonal antibodies to acetylcholinesterase (AChE) produced ipsilateral disappearance of AChE-positive nerve terminals within striatum and adjacent cortex. No alterations in striatal staining patterns were observed for tyrosine hydroxylase, somatostatin, neuropeptide Y, substance P, or neurotensin. Ultrastructural studies demonstrated the presence of degenerating AChE-positive boutons ipsilaterally, while tyrosine hydroxylase positive terminals seemed unaffected. Apomorphine administration to rats which had received unilateral antibody injection resulted in ipsilateral rotational behavior. These data suggest that selective effects on cholinergic terminals with functional deficits can be produced within the central nervous system by intracerebral injection of AChE antibodies.

KW - Acetylcholine

KW - Dopamine

KW - Rat

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KW - Tyrosine hydroxylase

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Effect of intracerebral injection of monoclonal acetylcholinesterase antibodies on cholinergic nerve terminals in the rat central nervous system

Abstract

Keywords

ASJC Scopus subject areas

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