Abstract
The authors examined the effects of both intermittent reperfusion and nitric oxide synthase (NOS) inhibition, caused by N(G)-nitro-L-arginine methyl ester (L-NAME) during episodes of focal cerebral ischemia induced to simulate the neurosurgical setting. Seventy-eight Wistar rats underwent single (60 minutes of ischemia) or repetitive (four 15-minute periods of ischemia separated by 5 minutes of reperfusion) episodes of middle cerebral artery occlusion while under anesthesia (1.0% halothane). Twenty-four hours after the procedure, the animals were given neurological examinations and then sacrificed for histological preparation and examination. The intermittent reperfusion groups tended to have smaller mean cortical infarctions. There was also a trend showing a decrease in infarction size in groups given L-NAME. The combination of intermittent reperfusion and preischemic administration of L-NAME (10 mg/kg) resulted in a 65% reduction in infarction size (p < 0.05) when compared to that caused by 60 minutes of single occlusion without L-NAME. The use of NOS inhibition combined with intermittent reperfusion may be a technique to provide intraoperative cerebral protection during neurovascular procedures that require temporary vascular occlusion.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 491-495 |
| Number of pages | 5 |
| Journal | Journal of Neurosurgery |
| Volume | 83 |
| Issue number | 3 |
| State | Published - 1995 |
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Keywords
- cerebral protection
- focal cerebral ischemia
- infarction
- nitric oxide
- rat
- reperfusion
ASJC Scopus subject areas
- Clinical Neurology
- Neuroscience(all)
Cite this
Effect of intermittent reperfusion and nitric oxide synthase inhibition on infarct volume during reversible focal cerebral ischemia. / Iuliano, B. A.; Anderson, R. E.; Meyer, F. B.
In: Journal of Neurosurgery, Vol. 83, No. 3, 1995, p. 491-495.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of intermittent reperfusion and nitric oxide synthase inhibition on infarct volume during reversible focal cerebral ischemia
AU - Iuliano, B. A.
AU - Anderson, R. E.
AU - Meyer, F. B.
PY - 1995
Y1 - 1995
N2 - The authors examined the effects of both intermittent reperfusion and nitric oxide synthase (NOS) inhibition, caused by N(G)-nitro-L-arginine methyl ester (L-NAME) during episodes of focal cerebral ischemia induced to simulate the neurosurgical setting. Seventy-eight Wistar rats underwent single (60 minutes of ischemia) or repetitive (four 15-minute periods of ischemia separated by 5 minutes of reperfusion) episodes of middle cerebral artery occlusion while under anesthesia (1.0% halothane). Twenty-four hours after the procedure, the animals were given neurological examinations and then sacrificed for histological preparation and examination. The intermittent reperfusion groups tended to have smaller mean cortical infarctions. There was also a trend showing a decrease in infarction size in groups given L-NAME. The combination of intermittent reperfusion and preischemic administration of L-NAME (10 mg/kg) resulted in a 65% reduction in infarction size (p < 0.05) when compared to that caused by 60 minutes of single occlusion without L-NAME. The use of NOS inhibition combined with intermittent reperfusion may be a technique to provide intraoperative cerebral protection during neurovascular procedures that require temporary vascular occlusion.
AB - The authors examined the effects of both intermittent reperfusion and nitric oxide synthase (NOS) inhibition, caused by N(G)-nitro-L-arginine methyl ester (L-NAME) during episodes of focal cerebral ischemia induced to simulate the neurosurgical setting. Seventy-eight Wistar rats underwent single (60 minutes of ischemia) or repetitive (four 15-minute periods of ischemia separated by 5 minutes of reperfusion) episodes of middle cerebral artery occlusion while under anesthesia (1.0% halothane). Twenty-four hours after the procedure, the animals were given neurological examinations and then sacrificed for histological preparation and examination. The intermittent reperfusion groups tended to have smaller mean cortical infarctions. There was also a trend showing a decrease in infarction size in groups given L-NAME. The combination of intermittent reperfusion and preischemic administration of L-NAME (10 mg/kg) resulted in a 65% reduction in infarction size (p < 0.05) when compared to that caused by 60 minutes of single occlusion without L-NAME. The use of NOS inhibition combined with intermittent reperfusion may be a technique to provide intraoperative cerebral protection during neurovascular procedures that require temporary vascular occlusion.
KW - cerebral protection
KW - focal cerebral ischemia
KW - infarction
KW - nitric oxide
KW - rat
KW - reperfusion
UR - http://www.scopus.com/inward/record.url?scp=0029143739&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029143739&partnerID=8YFLogxK
M3 - Article
C2 - 7666228
AN - SCOPUS:0029143739
VL - 83
SP - 491
EP - 495
JO - Journal of Neurosurgery
JF - Journal of Neurosurgery
SN - 0022-3085
IS - 3
ER -