Effect of functionalized micropatterned PLGA on guided neurite growth

Li Yao, Shenguo Wang, Wenjin Cui, Richard Sherlock, Claire O'Connell, Gopinath Damodaran, Adrienne Gorman, Anthony John Windebank, Abhay Pandit

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

When coaptation is not possible in the repair of nerve injuries, a bridge of biomaterial scaffold provides a structural support for neuronal cell growth and guides nerve regeneration. Poly(lactide-co-glycolide) (PLGA) scaffolds have been widely investigated for neural tissue engineering applications. In order to investigate guided neurite growth, we have fabricated micropatterns on PLGA films using laser ablation methods. The micropatterned PLGA films were coated with collagen type I or laminin peptide (PPFLMLLKGSTR) to promote axon growth. Micropatterned PLGA films provide a guidance effect on both early stage neurite outgrowth and elongation. Small (5 μm) grooves showed more statistically significant parallel neurite growth compared with larger size grooves (10 μm). Micropatterned PLGA films coated with laminin peptide showed more parallel neurite growth compared with those coated with collagen type I. Primary neurite number and total neurite length per cell decreased on micropatterned PLGA films compared with the controls. Neurites showed a preference for growth in the microgrooves rather than on the spaces. This study indicates that surface micropatterned structures with conjugated functional molecules can be used to guide neurite growth.

Original languageEnglish (US)
Pages (from-to)580-588
Number of pages9
JournalActa Biomaterialia
Volume5
Issue number2
DOIs
StatePublished - Feb 2009

Fingerprint

Neurites
Growth
Laminin
Scaffolds (biology)
Collagen Type I
Collagen
Peptides
Polyglactin 910
Cell growth
Biocompatible Materials
Laser ablation
Nerve Regeneration
Tissue engineering
Biomaterials
Laser Therapy
Tissue Engineering
polylactic acid-polyglycolic acid copolymer
Elongation
Repair
Axons

Keywords

  • Guided regeneration
  • Laminin peptide
  • Micropatterning
  • PLGA

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Yao, L., Wang, S., Cui, W., Sherlock, R., O'Connell, C., Damodaran, G., ... Pandit, A. (2009). Effect of functionalized micropatterned PLGA on guided neurite growth. Acta Biomaterialia, 5(2), 580-588. https://doi.org/10.1016/j.actbio.2008.09.002

Effect of functionalized micropatterned PLGA on guided neurite growth. / Yao, Li; Wang, Shenguo; Cui, Wenjin; Sherlock, Richard; O'Connell, Claire; Damodaran, Gopinath; Gorman, Adrienne; Windebank, Anthony John; Pandit, Abhay.

In: Acta Biomaterialia, Vol. 5, No. 2, 02.2009, p. 580-588.

Research output: Contribution to journalArticle

Yao, L, Wang, S, Cui, W, Sherlock, R, O'Connell, C, Damodaran, G, Gorman, A, Windebank, AJ & Pandit, A 2009, 'Effect of functionalized micropatterned PLGA on guided neurite growth', Acta Biomaterialia, vol. 5, no. 2, pp. 580-588. https://doi.org/10.1016/j.actbio.2008.09.002
Yao L, Wang S, Cui W, Sherlock R, O'Connell C, Damodaran G et al. Effect of functionalized micropatterned PLGA on guided neurite growth. Acta Biomaterialia. 2009 Feb;5(2):580-588. https://doi.org/10.1016/j.actbio.2008.09.002
Yao, Li ; Wang, Shenguo ; Cui, Wenjin ; Sherlock, Richard ; O'Connell, Claire ; Damodaran, Gopinath ; Gorman, Adrienne ; Windebank, Anthony John ; Pandit, Abhay. / Effect of functionalized micropatterned PLGA on guided neurite growth. In: Acta Biomaterialia. 2009 ; Vol. 5, No. 2. pp. 580-588.
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