Effect of fibrillation on acetylcholinesterase mRNA in cultured embryonic rat myotubes

L. H. Younkin, C. F. McTiernan, S. G. Younkin

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Acetylcholinesterase (AChE) and AChE mRNA were evaluated in spontaneously fibrillating myotubes derived from 20-day-old rat fetuses and in matched cultures in which fibrillation was prevented by adding tetrodotoxin on the fourth day of culture. On the eighth day of culture, the AChE activity of fibrillating and nonfibrillating cultures was 5332 and 1861 pmol ACh hydrolyzed min-1 dish-1, respectively (P < 0.005). Total mRNA was essentially the same in fibrillating and nonfibrillating cultures (27.4 and 25.4 μg/dish, respectively). AChE mRNA was assessed by assaying the AChE produced by Xenopus oocytes microinjected with purified mRNA. The AChE produced by mRNA from fibrillating and nonfibrillating cultures was 0.46 and 0.10 pmol ACh hydrolyzed min-1 oocyte-1, respectively (P < 0.005).

Original languageEnglish (US)
Pages (from-to)279-281
Number of pages3
JournalExperimental Cell Research
Volume174
Issue number1
DOIs
StatePublished - Jan 1988

ASJC Scopus subject areas

  • Cell Biology

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