Effect of electrical current on the activities of antimicrobial agents against Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus epidermidis biofilms

Jose L. Del Pozo, Mark S. Rouse, Jayawant Mandrekar, Marta Fernandez Sampedro, James M. Steckelberg, Robin Patel

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Bacterial biofilms are resistant to conventional antimicrobial agents. Prior in vitro studies have shown that electrical current (EC) enhances the activities of aminoglycosides, quinolones, and oxytetracycline against Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus epidermidis, Escherichia coli, and Streptococcus gordonii. This phenomenon, known as the bioelectric effect, has been only partially defined. The purpose of this work was to study the in vitro bioelectric effect on the activities of 11 antimicrobial agents representing a variety of different classes against P. aeruginosa, methicillin-resistant Staphylococcus aureus (MRSA), and S. epidermidis. An eight-channel current generator/controller and eight chambers delivering a continuous flow of fresh medium with or without antimicrobial agents and/or EC to biofilm-coated coupons were used. No significant decreases in the numbers of log10 CFU/cm2 were seen after exposure to antimicrobial agents alone, with the exception of a 4.57-log-unit reduction for S. epidermidis and trimethoprim-sulfamethoxazole. We detected a statistically significant bioelectric effect when vancomycin plus 2,000 microamperes EC were used against MRSA biofilms (P = 0.04) and when daptomycin and erythromycin were used in combination with 200 or 2,000 microamperes EC against S. epidermidis biofilms (P = 0.02 and 0.0004, respectively). The results of these experiments indicate that the enhancement of the activity of antimicrobial agents against biofilm organisms by EC is not a generalizable phenomenon across microorganisms and antimicrobial agents.

Original languageEnglish (US)
Pages (from-to)35-40
Number of pages6
JournalAntimicrobial Agents and Chemotherapy
Volume53
Issue number1
DOIs
StatePublished - Jan 2009

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Staphylococcus epidermidis
Biofilms
Anti-Infective Agents
Pseudomonas aeruginosa
Staphylococcus aureus
Methicillin-Resistant Staphylococcus aureus
Streptococcus gordonii
Daptomycin
Oxytetracycline
Quinolones
Sulfamethoxazole Drug Combination Trimethoprim
Klebsiella pneumoniae
Aminoglycosides
Erythromycin
Vancomycin
Escherichia coli

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology
  • Infectious Diseases

Cite this

Effect of electrical current on the activities of antimicrobial agents against Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus epidermidis biofilms. / Del Pozo, Jose L.; Rouse, Mark S.; Mandrekar, Jayawant; Sampedro, Marta Fernandez; Steckelberg, James M.; Patel, Robin.

In: Antimicrobial Agents and Chemotherapy, Vol. 53, No. 1, 01.2009, p. 35-40.

Research output: Contribution to journalArticle

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