Effect of electrical charge on chondrocyte attachment

M. Pumberger, M. Dadsetan, M. J. Yaszemski

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Our goal in this study is to develop hydrogels which serve as a substrate for chondrocyte attachment, proliferation and differentiation. We used oligo(poly(ethylene glycol) fumarate (OPF) as matrix of the hydrogels and incoporated charged molecules, sodium 2-Sulfoethyl methacrylate (SEMA) for negative and [2-(methacryloyloxy) ethyl)-trimethylammonium chloride (MAET AC) for positive charge. Swelling ratio of hydrogels was measured in PBS and deionized water at 37°C. In general, the negatively charged hydrogels showed a higher swelling ratio than positive or neutral charged. Both negatively and positively charged hydrogels had higher swelling ratio in deionized water as opposed to the PBS. Further we characterized the surface chemical structure of the hydrogels using Attenuated Total Reflectance (ATR) Fourier Transform Infrared (FTIR) Spectroscopy. The results showed incorporation of the charged molecules on the surface of the hydrogels. To investigate the effect of electrical charge on chondrocyte attachment, chondrocytes were seeded on the hydrogels at the density of 20,000 cells/cm2. At desired time points, cells were stained for viability and morphology. Our results showed that chondrocyte behavior is influenced by the type and density of electrical charge incorporated into the hydrogels.

Original languageEnglish (US)
Title of host publicationNEBEC 2009 - Proceedings of the IEEE 35th Annual Northeast Bioengineering Conference
DOIs
StatePublished - Sep 21 2009
EventIEEE 35th Annual Northeast Bioengineering Conference, NEBEC 2009 - Boston, MA, United States
Duration: Apr 3 2009Apr 5 2009

Publication series

NameBioengineering, Proceedings of the Northeast Conference
ISSN (Print)1071-121X

Other

OtherIEEE 35th Annual Northeast Bioengineering Conference, NEBEC 2009
CountryUnited States
CityBoston, MA
Period4/3/094/5/09

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ASJC Scopus subject areas

  • Bioengineering

Cite this

Pumberger, M., Dadsetan, M., & Yaszemski, M. J. (2009). Effect of electrical charge on chondrocyte attachment. In NEBEC 2009 - Proceedings of the IEEE 35th Annual Northeast Bioengineering Conference [4967738] (Bioengineering, Proceedings of the Northeast Conference). https://doi.org/10.1109/NEBC.2009.4967738