Effect of DL-α-lipoic acid on mitochondrial enzymes in aged rats

Palaniyappan Arivazhagan, Kadirvel Ramanathan, Chinnakkannu Panneerselvam

Research output: Contribution to journalArticlepeer-review

83 Scopus citations

Abstract

Mitochondrial dysfunction appears to contribute to some of the loss of function accompanying ageing. Mitochondria from aged tissue use oxygen inefficiently impairing ATP synthesis and results in increased oxidant production. A high flux of oxidants not only damages mitochondria, but other important cell biomolecules as well. In the present investigation, the levels of lipid peroxidation, oxidized glutathione, non-enzymatic antioxidants and the activities of mitochondrial enzymes were measured in liver and kidney mitochondria of young and aged rats before and after lipoic acid supplementation. In both liver and kidney increase in the levels of mitochondrial lipid peroxidation and oxidized glutathione and decrease in the levels of antioxidants and the activities of mitochondrial enzymes were observed in aged rats. DL-α-lipoic acid supplemented aged rats showed a decrease in the levels of lipid peroxidation and oxidized glutathione and increase in the levels of reduced glutathione, vitamins C and E and the activities of mitochondrial enzymes like isocitrate dehydrogenase, α-ketoglutarate dehydrogenase, succinate dehydrogenase, NADH-dehydrogenase and cytochrome-c-oxidase. Thus, lipoic acid reverses the age-associated decline in endogenous low molecular weight antioxidants and mitochondrial enzymes and, therefore, may lower the increased risk of oxidative damage that occurs during ageing. From our results it can be concluded that lipoic acid supplementation enhances the activities of mitochondrial enzymes and antioxidant status and thereby protects mitochondria from ageing.

Original languageEnglish (US)
Pages (from-to)189-198
Number of pages10
JournalChemico-biological interactions
Volume138
Issue number2
DOIs
StatePublished - Nov 28 2001

Keywords

  • Ageing and DL-α-lipoic acid
  • Antioxidants
  • Lipid peroxidation
  • Mitochondria

ASJC Scopus subject areas

  • Toxicology

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