Effect of cortisol on [3H] 1,25-dihydroxyvitamin D3 uptake and 1,25-dihydroxyvitamin D3-induced DNA-dependent RNA polymerase activity in chick intestinal cells

Terry D. Shultz, Rajiv Kumar

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The influence of cortisol on intestinal DNA-dependent RNA polymerase activity was studied in purified nuclei of vitamin D-deficient or 1,25-dihydroxyvitamin D3-treated chicks. Six- to 7-week-old vitamin D-deficient cockerels were given 5 mg of cortisol or vehicle intraperitoneally 24 and 48 hours before sacrifice. Three hours before sacrifice, 200 ng of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) was administered intracardially. Cortisol did not alter the uptake or metabolism of 1,25(OH)2D3 in the intestinal mucosa. After a 200 ng dose of 1,25(OH)2D3 the in situ intestinal ligated loop technique revealed a 39% increase in calcium absorption compared to control birds (P<0.001). The administration of cortisol (5 mg) to chickens given 1,25(OH)2D3, however, resulted in a significant decrease in intestinal calcium transport in vivo (P<0.0025). When intestinal nuclei were prepared from birds treated in a manner identical with that described above, 1,25(OH)2D3-treated and 1,25(OH)2D3 plus cortisol-treated chicks had intestinal RNA polymerase II transcriptional activities that were significantly greater than those of vitamin D-deficient controls (P≤0.02, P≤0.005). There was no difference between RNA polymerase II and I+III activities of the 1,25(OH)2D3-treated birds and that of the cortisol plus 1,25(OH)2D3-treated birds. Vitamin D-deficient chicks treated with cortisol alone showed RNA polymerase I+III activity that was significantly higher (P≤0.01) than that of birds treated with vehicle alone. As transcription is not altered by cortisol following 1,25(OH)2D3 treatment, we suggest that cortisol may inhibit intestinal calcium transport by altering posttranscriptional or other events such as basolateral membrane calcium transport.

Original languageEnglish (US)
Pages (from-to)224-230
Number of pages7
JournalCalcified Tissue International
Volume40
Issue number4
DOIs
StatePublished - Jul 1987

Fingerprint

Calcitriol
DNA-Directed RNA Polymerases
Hydrocortisone
Birds
Vitamin D
RNA Polymerase I
Calcium
RNA Polymerase II
RNA Polymerase III
Intestinal Mucosa
Chickens
Membranes

Keywords

  • 1,25(OH)D
  • Calcium transport
  • Calcium-binding protein
  • Cortisol
  • RNA polymerase

ASJC Scopus subject areas

  • Endocrinology
  • Orthopedics and Sports Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

@article{197253415f984893a90b7b005574d829,
title = "Effect of cortisol on [3H] 1,25-dihydroxyvitamin D3 uptake and 1,25-dihydroxyvitamin D3-induced DNA-dependent RNA polymerase activity in chick intestinal cells",
abstract = "The influence of cortisol on intestinal DNA-dependent RNA polymerase activity was studied in purified nuclei of vitamin D-deficient or 1,25-dihydroxyvitamin D3-treated chicks. Six- to 7-week-old vitamin D-deficient cockerels were given 5 mg of cortisol or vehicle intraperitoneally 24 and 48 hours before sacrifice. Three hours before sacrifice, 200 ng of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) was administered intracardially. Cortisol did not alter the uptake or metabolism of 1,25(OH)2D3 in the intestinal mucosa. After a 200 ng dose of 1,25(OH)2D3 the in situ intestinal ligated loop technique revealed a 39{\%} increase in calcium absorption compared to control birds (P<0.001). The administration of cortisol (5 mg) to chickens given 1,25(OH)2D3, however, resulted in a significant decrease in intestinal calcium transport in vivo (P<0.0025). When intestinal nuclei were prepared from birds treated in a manner identical with that described above, 1,25(OH)2D3-treated and 1,25(OH)2D3 plus cortisol-treated chicks had intestinal RNA polymerase II transcriptional activities that were significantly greater than those of vitamin D-deficient controls (P≤0.02, P≤0.005). There was no difference between RNA polymerase II and I+III activities of the 1,25(OH)2D3-treated birds and that of the cortisol plus 1,25(OH)2D3-treated birds. Vitamin D-deficient chicks treated with cortisol alone showed RNA polymerase I+III activity that was significantly higher (P≤0.01) than that of birds treated with vehicle alone. As transcription is not altered by cortisol following 1,25(OH)2D3 treatment, we suggest that cortisol may inhibit intestinal calcium transport by altering posttranscriptional or other events such as basolateral membrane calcium transport.",
keywords = "1,25(OH)D, Calcium transport, Calcium-binding protein, Cortisol, RNA polymerase",
author = "Shultz, {Terry D.} and Rajiv Kumar",
year = "1987",
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N2 - The influence of cortisol on intestinal DNA-dependent RNA polymerase activity was studied in purified nuclei of vitamin D-deficient or 1,25-dihydroxyvitamin D3-treated chicks. Six- to 7-week-old vitamin D-deficient cockerels were given 5 mg of cortisol or vehicle intraperitoneally 24 and 48 hours before sacrifice. Three hours before sacrifice, 200 ng of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) was administered intracardially. Cortisol did not alter the uptake or metabolism of 1,25(OH)2D3 in the intestinal mucosa. After a 200 ng dose of 1,25(OH)2D3 the in situ intestinal ligated loop technique revealed a 39% increase in calcium absorption compared to control birds (P<0.001). The administration of cortisol (5 mg) to chickens given 1,25(OH)2D3, however, resulted in a significant decrease in intestinal calcium transport in vivo (P<0.0025). When intestinal nuclei were prepared from birds treated in a manner identical with that described above, 1,25(OH)2D3-treated and 1,25(OH)2D3 plus cortisol-treated chicks had intestinal RNA polymerase II transcriptional activities that were significantly greater than those of vitamin D-deficient controls (P≤0.02, P≤0.005). There was no difference between RNA polymerase II and I+III activities of the 1,25(OH)2D3-treated birds and that of the cortisol plus 1,25(OH)2D3-treated birds. Vitamin D-deficient chicks treated with cortisol alone showed RNA polymerase I+III activity that was significantly higher (P≤0.01) than that of birds treated with vehicle alone. As transcription is not altered by cortisol following 1,25(OH)2D3 treatment, we suggest that cortisol may inhibit intestinal calcium transport by altering posttranscriptional or other events such as basolateral membrane calcium transport.

AB - The influence of cortisol on intestinal DNA-dependent RNA polymerase activity was studied in purified nuclei of vitamin D-deficient or 1,25-dihydroxyvitamin D3-treated chicks. Six- to 7-week-old vitamin D-deficient cockerels were given 5 mg of cortisol or vehicle intraperitoneally 24 and 48 hours before sacrifice. Three hours before sacrifice, 200 ng of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) was administered intracardially. Cortisol did not alter the uptake or metabolism of 1,25(OH)2D3 in the intestinal mucosa. After a 200 ng dose of 1,25(OH)2D3 the in situ intestinal ligated loop technique revealed a 39% increase in calcium absorption compared to control birds (P<0.001). The administration of cortisol (5 mg) to chickens given 1,25(OH)2D3, however, resulted in a significant decrease in intestinal calcium transport in vivo (P<0.0025). When intestinal nuclei were prepared from birds treated in a manner identical with that described above, 1,25(OH)2D3-treated and 1,25(OH)2D3 plus cortisol-treated chicks had intestinal RNA polymerase II transcriptional activities that were significantly greater than those of vitamin D-deficient controls (P≤0.02, P≤0.005). There was no difference between RNA polymerase II and I+III activities of the 1,25(OH)2D3-treated birds and that of the cortisol plus 1,25(OH)2D3-treated birds. Vitamin D-deficient chicks treated with cortisol alone showed RNA polymerase I+III activity that was significantly higher (P≤0.01) than that of birds treated with vehicle alone. As transcription is not altered by cortisol following 1,25(OH)2D3 treatment, we suggest that cortisol may inhibit intestinal calcium transport by altering posttranscriptional or other events such as basolateral membrane calcium transport.

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